EMAGE mouse gene expression spatial database

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EMAGE:10085

Palm paralemmin ( MGI:1261814)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000027. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000027_01	euxassay_000027_02	euxassay_000027_03	euxassay_000027_04

euxassay_000027_05	euxassay_000027_06	euxassay_000027_07	euxassay_000027_08	euxassay_000027_09

euxassay_000027_10	euxassay_000027_11	euxassay_000027_12	euxassay_000027_13	euxassay_000027_14

euxassay_000027_15	euxassay_000027_16	euxassay_000027_17	euxassay_000027_18	euxassay_000027_19

euxassay_000027_20	euxassay_000027_21	euxassay_000027_22	euxassay_000027_23	euxassay_000027_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10085_wholemount_strong.wlz

10085_wholemount_moderate.wlz

10085_wholemount_weak.wlz

10085_wholemount_possible.wlz

10085_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10085_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

central nervous system

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

central nervous system ganglion

strong

homogeneous

strong expression: see section 09 10 19 20 21

cranial ganglion

strong

homogeneous

strong expression: see section 08 19 20 21

facial vii ganglion

strong

homogeneous

strong expression: see section 06 07 22 23

trigeminal v ganglion

strong

homogeneous

strong expression: see section 04 05 06 07 22 23 24

vestibulocochlear viii ganglion

strong

homogeneous

strong expression: see section 07 22

central nervous system nerve

strong

homogeneous

strong expression: see section 10

peripheral nervous system

strong

homogeneous

strong expression: see section 15 16 17 18 19 20 21

autonomic nervous system

strong

homogeneous

strong expression: see section 11 12 13 14 15 16 17 18 19 20 21 22 moderate expression: see section 06

spinal component of peripheral nervous system

strong

homogeneous

strong expression: see section 11 12 13 14 18 19 20 21

retina

moderate

homogeneous

moderate expression: see section 03 04

nasal cavity olfactory epithelium

moderate

homogeneous

moderate expression: see section 10 11 12 13 16 17 18 19 20

vomeronasal organ epithelium

moderate

homogeneous

moderate expression: see section 16

liver

moderate

homogeneous

moderate expression: see section 03 weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 18 19 20 21 23 24

renal cortex

strong

spotted

strong expression: see section 09 10 11 12 19 20 moderate expression: see section 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1711
Entity Detected:	Palm, paralemmin ( MGI:1261814)
Sequence:	sense strand is shown >T1711 TGGCCTCGAGCCAGATTCGGACGAGGGTGGAGTGAGGCCACAGTTCTTTGGGGACTCAAAGTCTTGAAGT TCTAGGGAGGAGGATGGGCACCTGTGGATCCCCTCCCATAGGTAGCAGAGCTCAAAGCAGGGGAAACTCT TCACCGGGGATAGAACAATCACCCGCAGGATGGAGTCATTGTTGGCCAGCCAAGCCACTCCCTGTCCTGA GTCCCACCCCCACCCCCACCATGCACACACTGGGCCCTTCTTCCTGGTGCTAATTTTGGGAGCTTTGGGG TCACCTCTGCCCTCTTCTCTAGTTGGTTTGGACCAGGGTCCTGGGTTTCCCACATACCCCCATACCCTAG AAGTCAGTGGGTATGTCTGCCAGCGCCACAAAACCTAGCATATCCAGGCAGGTTTAGGCTGCTCCACACA CACTTTGGGTGGGAGTAAGGGACCCCCCCCCATCTGCCACCTGGCCTGACGGGAGCCTGGCAGAGGGTCA CCAACCTTCGGGCAGGCAGCAGTAGCCACTTTGGTCCCCAGAGGCACAACCCTGCACACAGGAGCTGACT CAAC
Notes:	The probe template was PCR amplified from IMAGE:478371 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:478371 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10084 same embryo
	EurExpress:euxassay_000027 same experiment
	MGI:4827033 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source