EMAGE mouse gene expression spatial database

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EMAGE:10101

Zfp184 zinc finger protein 184 (Kruppel-like) ( MGI:1922244)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000250. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000250_01	euxassay_000250_02	euxassay_000250_03	euxassay_000250_04

euxassay_000250_05	euxassay_000250_06	euxassay_000250_07	euxassay_000250_08	euxassay_000250_09

euxassay_000250_10	euxassay_000250_11	euxassay_000250_12	euxassay_000250_13	euxassay_000250_14

euxassay_000250_15	euxassay_000250_16	euxassay_000250_17	euxassay_000250_18	euxassay_000250_19

euxassay_000250_20	euxassay_000250_21	euxassay_000250_22	euxassay_000250_23	euxassay_000250_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10101_wholemount_strong.wlz

10101_wholemount_moderate.wlz

10101_wholemount_weak.wlz

10101_wholemount_possible.wlz

10101_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10101_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 06 15 16 17 18

vibrissa

moderate

regional

moderate expression: see section 03 06 21

cerebral cortex mantle layer

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22

esophagus

weak

regional

weak expression: see section 11 12

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 14

lower jaw molar

moderate

regional

moderate expression: see section 05 06 19

upper jaw incisor

moderate

regional

moderate expression: see section 10 11 12

upper jaw molar

moderate

regional

moderate expression: see section 05 06 19

renal cortex

moderate

regional

moderate expression: see section 08 09 10 11 12 18 19 20 21 22

lung

moderate

spotted

moderate expression: see section 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22

trachea

weak

regional

weak expression: see section 11 12 13

tail dorsal root ganglion

weak

regional

weak expression: see section 05 06 07 08 10 14 15 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1549
Entity Detected:	Zfp184, zinc finger protein 184 (Kruppel-like) ( MGI:1922244)
Sequence:	sense strand is shown >T1549 TTTTTTTTTTTTTTGTAGTGCAACTATTTATGAATATAAATATACAAAACTGAGTATAAAACTCCAAGCT ACCTTTCAGATCATCTCTGAATCTTTCATTTCACCTCATTTACAGAAGAAACTTAAAGCAGCTTGCCACA ATGTTGTTTTAATAACATACTTGGATCAGAACAGCCCAATGGGCTCTCCATCCCACCATGTGACAGAGAA AAATTTACTTTAAGGAGTTCCTCCTCCCACTCTGATTCATTTCTATAAAACCAAAGTTAGCAGAGCAACT ATGTTAATGATGTTCCTACAGCAGCCACAGCAGGGTGCAATCTCTGGTGTTTGGTGAGAGCAGAGTGACA CTGGAAGGCTTTTTCACAGTCCTCACACCCCAAAAGCTTCTCTCCTGAATGAATCTTCTGATGCTGAGTG AGGTATGTGCTCTGGCTGAAAGTCTGTGGGCACTCAGAGTTGTAAGGTTTCTCTCCAGTGTGAGTACTCC GATGTTCAGTCAGGTGCA
Notes:	The probe template was PCR amplified from IMAGE:865559 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:865559 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10100 same embryo
	EMAGE:10099 same embryo
	EMAGE:10102 same embryo
	EMAGE:10103 same embryo
	EurExpress:euxassay_000250 same experiment
	MGI:4829293 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source