EMAGE mouse gene expression spatial database

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EMAGE:10110

Cep68 centrosomal protein 68 ( MGI:2667663)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000074. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000074_01	euxassay_000074_02	euxassay_000074_03	euxassay_000074_04

euxassay_000074_05	euxassay_000074_06	euxassay_000074_07	euxassay_000074_08	euxassay_000074_09

euxassay_000074_10	euxassay_000074_11	euxassay_000074_12	euxassay_000074_13	euxassay_000074_14

euxassay_000074_15	euxassay_000074_16	euxassay_000074_17	euxassay_000074_18	euxassay_000074_19

euxassay_000074_20	euxassay_000074_21	euxassay_000074_22	euxassay_000074_23	euxassay_000074_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10110_wholemount_strong.wlz

10110_wholemount_moderate.wlz

10110_wholemount_weak.wlz

10110_wholemount_possible.wlz

10110_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10110_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 13 14 17 18 weak expression: see section 15 16

submandibular gland primordium

strong

regional

strong expression: see section 09 10 11 12 19 20 21 22 moderate expression: see section 08

vibrissa

weak

regional

weak expression: see section 06 07 08 21 22

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

moderate

homogeneous

moderate expression: see section 05 06 weak expression: see section 07 08 23

glossopharyngeal ix ganglion

weak

homogeneous

weak expression: see section 21

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 05 06 weak expression: see section 07 08 09 10 19 20 21 22 23 24

lower jaw incisor

weak

regional

weak expression: see section 12 13 14 17 18

upper jaw incisor

weak

regional

weak expression: see section 12 13 14 16 17

renal cortex

weak

regional

weak expression: see section 09 10 11 12 13 19 20 21 22 23 24

testis

weak

regional

weak expression: see section 07 08 09 10 22 23 24

lung

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tail dorsal root ganglion

moderate

regional

moderate expression: see section 18 21 weak expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1517
Entity Detected:	Cep68, centrosomal protein 68 ( MGI:2667663)
Sequence:	sense strand is shown >T1517 TCCCTCCTTTCAGGCAGAATACTGGGCCTGTGCGCTGCCAAATTCTCTGCCTCCTTCCCCTAACCGCCAC TCCGCACTCTGGGACCCAAATAAAGAGTATGAAGATCTGCTTGACTACACTTACCCACTGAGGCCTGGGC CTCAGCTCCCAAAGCAACCTGAAAGTCATGTCCTGACTGAGCCTGTTCTGCAGGACTCAGGTGTAGATCT GGACAGTTTGTCTGTCTCCCCAGCAAGTACTCTAAAGTCACCCACTAATGTCTCCCACAATTGCTCATCA GCAGAAGTGCCTACTCTGCCATTTTCTGGAGCCAGAGAGTCATGTCTTAAGCGCTGGCCCTTGGGAATAT TCCAGAAACAGGGTGGCACAAGCTTGTCATCCTGGAACCAGCTTGCATCAACCCCTAGAGCCCCAGGCAC TGAAGATGCTTCTTGGGAGAACAGAGAGGCAGCCCTGAGGGGCACAGCGGAGGACTGCCTCCCTATAGGT GAGGACCTCCGAATGGGCTCTCCCCAGCTG
Notes:	The probe template was PCR amplified from IMAGE:790035 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:790035 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10111 same embryo
	EMAGE:10109 same embryo
	EMAGE:10108 same embryo
	EurExpress:euxassay_000074 same experiment
	MGI:4823818 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source