EMAGE mouse gene expression spatial database

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EMAGE:10114

D15Ertd621e DNA segment, Chr 15, ERATO Doi 621, expressed ( MGI:1277178)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000052. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000052_01	euxassay_000052_02	euxassay_000052_03	euxassay_000052_04

euxassay_000052_05	euxassay_000052_06	euxassay_000052_07	euxassay_000052_08	euxassay_000052_09

euxassay_000052_10	euxassay_000052_11	euxassay_000052_12	euxassay_000052_13	euxassay_000052_14

euxassay_000052_15	euxassay_000052_16	euxassay_000052_17	euxassay_000052_18	euxassay_000052_19

euxassay_000052_20	euxassay_000052_21	euxassay_000052_22	euxassay_000052_23	euxassay_000052_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10114_wholemount_strong.wlz

10114_wholemount_moderate.wlz

10114_wholemount_weak.wlz

10114_wholemount_possible.wlz

10114_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10114_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 11 12 14 17 18 19 20 21 22 24

lumbar vertebral cartilage condensation

moderate

homogeneous

moderate expression: see section 10

thoracic intervertebral disc

moderate

homogeneous

moderate expression: see section 22

humerus

moderate

homogeneous

moderate expression: see section 01 02 23 24

carpus

moderate

homogeneous

moderate expression: see section 01

hindlimb digit 2 metatarsal

moderate

homogeneous

moderate expression: see section 24

hindlimb digit 3 metatarsal

moderate

homogeneous

moderate expression: see section 24

hindlimb digit 4 metatarsal

moderate

homogeneous

moderate expression: see section 24

fibula

moderate

homogeneous

moderate expression: see section 01 24

tibia

moderate

homogeneous

moderate expression: see section 01 24

femur

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 20 21 23 24

meckel's cartilage

moderate

homogeneous

moderate expression: see section 03 05 06 07 08 10 11 12 13 15 16 17 18 19 20 21 22 weak expression: see section 04

lower jaw mesenchyme

moderate

homogeneous

moderate expression: see section 23

cervical vertebra

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

lumbar vertebra

moderate

homogeneous

moderate expression: see section 09 11 12 13 14 15 16 17 18 20

sacral vertebral cartilage condensation

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 15 16 17

thoracic vertebra

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 15 16 17 18 19 20 21 24

exoccipital bone

weak

homogeneous

weak expression: see section 06

clavicle

moderate

homogeneous

moderate expression: see section 04 23 24

scapula

moderate

homogeneous

moderate expression: see section 04 23 24

pelvic girdle skeleton

moderate

homogeneous

moderate expression: see section 10 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1645
Entity Detected:	D15Ertd621e, DNA segment, Chr 15, ERATO Doi 621, expressed ( MGI:1277178)
Sequence:	sense strand is shown >T1645 TGGCCTCGAGCCAGATTCGGCACGAGGCTGAACTAGAAGCCAAATCAGGCTCGCCTCTCTCATCAGAAGC CTCACTGAGCTTCCTACTTGCAAGCTGGCTAGAAGCATTCAACATGGTGACATAGCCGCAGAAATGCTGC AAGTCCACTCTGCTCCGCAGAATCTGCAGTGCTTTATGAACACCCATACTGGCACGGGTGAACTCTCCTT GCANCTCTGCTTCATCAAATGGAAAGGGTATATGGACAGTCTCTCCTACCCCATGCAAACCATGTCCCTG AATGAGCACTGCTGAGTGGGTTAAAGCATCATTCAGCATCGTGAGAACGTTTGAAGCAGGAACGACCCCA GGGTCATGGCCCCAAGAGGTTATAAGTAACCGATCATAACCCTGGAATATATCTGGCAGTTTCCGAAGTC TTGTACCTTTGGACAGTAAGAGAGATGGTGGTCCTTGCCCAGTGACATGGTAAATATACAATTTAAACCA GACAGAACTGACTTCTGGGATAGCTGGTCCGATATGCTGCGGGGTGCAGCTGCTGACTGGCCGGATCTCA TTGGTAAGAG
Notes:	The probe template was PCR amplified from IMAGE:458320 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:458320 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10115 same embryo
	EMAGE:10113 same embryo
	EMAGE:10116 same embryo
	EMAGE:10112 same embryo
	EurExpress:euxassay_000052 same experiment
	MGI:4824196 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source