EMAGE mouse gene expression spatial database

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EMAGE:10135

Atp1b1 ATPase, Na+/K+ transporting, beta 1 polypeptide ( MGI:88108)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000015. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000015_01	euxassay_000015_02	euxassay_000015_03	euxassay_000015_04

euxassay_000015_05	euxassay_000015_06	euxassay_000015_07	euxassay_000015_08	euxassay_000015_09

euxassay_000015_10	euxassay_000015_11	euxassay_000015_12	euxassay_000015_13	euxassay_000015_14

euxassay_000015_15	euxassay_000015_16	euxassay_000015_17	euxassay_000015_18	euxassay_000015_19

euxassay_000015_20	euxassay_000015_21	euxassay_000015_22	euxassay_000015_23	euxassay_000015_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10135_wholemount_strong.wlz

10135_wholemount_moderate.wlz

10135_wholemount_weak.wlz

10135_wholemount_possible.wlz

10135_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10135_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pericardium

strong

homogeneous

strong expression: see section 24 moderate expression: see section 22

medulla oblongata part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 04 14 18

visceral organ system

strong

regional

strong expression: see section 06

pancreas

strong

homogeneous

strong expression: see section 10 11 12 13 19 moderate expression: see section 17

body of pancreas

strong

spotted

strong expression: see section 10 11 12 13

head of pancreas

strong

spotted

strong expression: see section 10 11 12 13

tail of pancreas

strong

spotted

strong expression: see section 13 19

forebrain

strong

single cell

strong expression: see section 12

pituitary gland

strong

single cell

strong expression: see section 10 11 12 moderate expression: see section 15

adenohypophysis

strong

single cell

strong expression: see section 12 13

adenohypophysis pars anterior

moderate

regional

moderate expression: see section 12 13 14 15 16

adenohypophysis pars intermedia

moderate

homogeneous

moderate expression: see section 13 14

adenohypophysis pars tuberalis

moderate

homogeneous

moderate expression: see section 13 14

neurohypophysis

strong

single cell

strong expression: see section 10 12 13 moderate expression: see section 15

neurohypophysis infundibulum

strong

regional

strong expression: see section 13 moderate expression: see section 14 15 16

median eminence

moderate

regional

moderate expression: see section 15 16

neurohypophysis

moderate

regional

moderate expression: see section 13 15 16

physiological umbilical hernia

strong

regional

strong expression: see section 11 12 14 18 19

diencephalic part of interventricular foramen

moderate

regional

moderate expression: see section 13

epithalamus

weak

regional

weak expression: see section 12

epithalamus ventricular layer

moderate

regional

moderate expression: see section 13 14

3rd ventricle choroid plexus

strong

regional

strong expression: see section 14 moderate expression: see section 13

hypothalamus

moderate

regional

moderate expression: see section 11

hypothalamus mantle layer

weak

regional

weak expression: see section 12

hypothalamus marginal layer

weak

regional

weak expression: see section 12

hypothalamus ventricular layer

moderate

homogeneous

moderate expression: see section 13 14 weak expression: see section 12 15 16

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 11

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 13 14 weak expression: see section 12 15 16

thalamus

weak

regional

weak expression: see section 10

thalamus mantle layer

weak

regional

weak expression: see section 12

thalamus ventricular layer

moderate

homogeneous

moderate expression: see section 11 13 14 weak expression: see section 15 16

telencephalon

strong

single cell

strong expression: see section 12 21

cerebral cortex

strong

homogeneous

strong expression: see section 02 03 04 07 12 14 18 21 moderate expression: see section 06 10 weak expression: see section 05 08

cerebral cortex mantle layer

strong

homogeneous

strong expression: see section 01 12 13 14 18 moderate expression: see section 15 16

cerebral cortex marginal layer

strong

homogeneous

strong expression: see section 11 12 13 14 18 moderate expression: see section 15 16

cerebral cortex ventricular layer

strong

spotted

strong expression: see section 21

lateral ventricle choroid plexus

strong

homogeneous

strong expression: see section 11 12

corpus striatum

weak

regional

weak expression: see section 04 07 08

hindbrain

moderate

regional

moderate expression: see section 10

medulla oblongata

strong

regional

strong expression: see section 06 07

medulla oblongata lateral wall

strong

spotted

strong expression: see section 21

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 12 moderate expression: see section 13 14 15 16

medulla oblongata alar plate marginal layer

moderate

regional

moderate expression: see section 14 15 16

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 12 moderate expression: see section 10 11 13 14 15 16

medulla oblongata basal plate marginal layer

moderate

regional

moderate expression: see section 14 15 16

cerebellum

strong

regional

strong expression: see section 02 03 04 12 13 21 22 moderate expression: see section 08 10 14 weak expression: see section 06 07

cerebellum intraventricular portion

strong

homogeneous

strong expression: see section 18

rest of cerebellum mantle layer

strong

homogeneous

strong expression: see section 12 13 14 15 16 moderate expression: see section 11

rest of cerebellum marginal layer

strong

homogeneous

strong expression: see section 12 13 14 15 16

metencephalon rest of alar plate

strong

homogeneous

strong expression: see section 18

metencephalon basal plate

strong

regional

strong expression: see section 06 07 moderate expression: see section 08 10

pons

strong

regional

strong expression: see section 07 12

pons mantle layer

strong

regional

strong expression: see section 12 moderate expression: see section 13 14 15 16

pons marginal layer

strong

regional

strong expression: see section 12 moderate expression: see section 13 14 15 16

metencephalon part of 4th ventricle choroid plexus

strong

homogeneous

strong expression: see section 11 12 13 14

midbrain

strong

regional

strong expression: see section 04 06 07 not examined expression: see section 10

midbrain mantle layer

strong

regional

strong expression: see section 10 11 12 13 14 moderate expression: see section 15 16

midbrain marginal layer

strong

regional

strong expression: see section 12 13 moderate expression: see section 15 16

tegmentum

strong

regional

strong expression: see section 12 moderate expression: see section 11 14 weak expression: see section 10

central nervous system ganglion

moderate

spotted

moderate expression: see section 07

facial vii ganglion

strong

homogeneous

strong expression: see section 04 07 08

glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 07 08

trigeminal v ganglion

strong

homogeneous

strong expression: see section 03 04 05 06 07 08 09 18 19 22 23

vagus x ganglion

strong

homogeneous

strong expression: see section 06 07 08 18 19

vestibulocochlear viii ganglion

strong

homogeneous

strong expression: see section 07 08 18

spinal cord lateral wall

strong

regional

strong expression: see section 14

spinal cord mantle layer

strong

homogeneous

strong expression: see section 13 14 moderate expression: see section 10

alar columns

moderate

homogeneous

moderate expression: see section 11 12 15 16

basal columns

moderate

homogeneous

moderate expression: see section 11 12 15 16

spinal cord marginal layer

strong

homogeneous

strong expression: see section 13 14

spinal cord sulcus limitans

strong

regional

strong expression: see section 14

autonomic nervous system

moderate

homogeneous

moderate expression: see section 08

hypogastric plexus

strong

homogeneous

strong expression: see section 13

sympathetic nervous system

strong

homogeneous

strong expression: see section 11

sympathetic ganglion

strong

homogeneous

strong expression: see section 11

thoracic ganglion

strong

homogeneous

strong expression: see section 12 14

spinal ganglion

strong

homogeneous

strong expression: see section 08

dorsal root ganglion

strong

homogeneous

strong expression: see section 11 14 16 17 18

nasal cavity olfactory epithelium

strong

homogeneous

strong expression: see section 11 moderate expression: see section 10 14 15 not examined expression: see section 12

nasal cavity respiratory epithelium

strong

homogeneous

strong expression: see section 11 moderate expression: see section 10 14 15 not examined expression: see section 12

nasal septum

weak

regional

weak expression: see section 13

nasal septum epithelium

weak

homogeneous

weak expression: see section 13

viscerocranium

moderate

regional

Expression in the turbinate bone.

heart

strong

homogeneous

strong expression: see section 13 24 moderate expression: see section 08 10 11 12 17 22 weak expression: see section 09

heart atrium

strong

homogeneous

strong expression: see section 12 13 14 15 16 20 moderate expression: see section 11 17 18 19

endocardial cushion tissue

strong

regional

strong expression: see section 14

endocardial tissue

strong

regional

strong expression: see section 14

interventricular groove

moderate

homogeneous

moderate expression: see section 13

left ventricle cardiac muscle

moderate

homogeneous

moderate expression: see section 14 15 16 17 18 19 not examined expression: see section 11 12

right ventricle cardiac muscle

moderate

homogeneous

moderate expression: see section 10 11 12

foregut duodenum

strong

homogeneous

strong expression: see section 14

duodenum rostral part

strong

homogeneous

strong expression: see section 14

duodenum rostral part epithelium

strong

homogeneous

strong expression: see section 13 14 15

gastro-esophageal junction

strong

homogeneous

strong expression: see section 14

gastro-esophageal junction epithelium

strong

homogeneous

strong expression: see section 14 15

stomach

strong

homogeneous

strong expression: see section 06 moderate expression: see section 03

stomach fundus

strong

regional

strong expression: see section 06

stomach pyloric region

strong

regional

strong expression: see section 06 07 08

stomach pyloric region epithelium

strong

homogeneous

strong expression: see section 10 11 12

pyloric antrum

strong

regional

strong expression: see section 12

not examined

regional

not examined expression: see section 06 07 08 10 11

perineal body epithelium

strong

homogeneous

strong expression: see section 15

rectum epithelium

strong

homogeneous

strong expression: see section 15

midgut

strong

homogeneous

strong expression: see section 15

duodenum caudal part epithelium

strong

homogeneous

strong expression: see section 15

jejunum epithelium

strong

homogeneous

strong expression: see section 15

metanephros

strong

spotted

strong expression: see section 11 19 20 22 moderate expression: see section 23 weak expression: see section 09

kidney calyx

strong

spotted

strong expression: see section 11 moderate expression: see section 07

collecting duct

strong

spotted

strong expression: see section 10 moderate expression: see section 07

metanephros

strong

spotted

strong expression: see section 20 21 22

renal cortex

strong

spotted

strong expression: see section 08 11

medullary renal tubule

moderate

spotted

moderate expression: see section 07

testis

strong

spotted

strong expression: see section 07 08 moderate expression: see section 24

testis

not examined

spotted

not examined expression: see section 10 11

respiratory system

strong

spotted

strong expression: see section 19

laryngeal cartilage

strong

regional

strong expression: see section 14

lung

strong

spotted

strong expression: see section 05 07 08 10 19 moderate expression: see section 12 14 18 22 23 24 weak expression: see section 11 21

respiratory tract

moderate

regional

moderate expression: see section 14

lower respiratory tract

moderate

regional

moderate expression: see section 14

upper respiratory tract

moderate

regional

moderate expression: see section 14 15

not examined

homogeneous

not examined expression: see section 14

stomach fundus lumen

strong

regional

strong expression: see section 06

stomach proventricular region lumen

strong

regional

strong expression: see section 06 09

stomach pyloric region lumen

strong

regional

strong expression: see section 06 09 12

foregut-midgut junction lumen

strong

regional

strong expression: see section 09 18

perineal body lumen

strong

homogeneous

strong expression: see section 20

rectum lumen

strong

regional

strong expression: see section 18 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1781
Entity Detected:	Atp1b1, ATPase, Na+/K+ transporting, beta 1 polypeptide ( MGI:88108)
Sequence:	sense strand is shown >T1781 TGGCCTCGAGGCCAGATTCGGATCCATGCTTGTAGTGAGCAGTGTTCTGGCCCCTAAGTATTGCCGCCTT GTCTATTTTATTTAGTGTACAGTACTATAGGTGCGCACTCTGGTCATTTTTCAAGCCATGTTTTATCATA TCTGTTTTCTACTTTACGTGAGCAAGGTTTGCTGTCCAAGGTGTAAATACTCAACGGGAATAAAACTGGC ATGGTACTTTTTCCTTTCTTTCTTATTTTCTTGGCTCTGAAATTTCAAAGGTAACGGCCCATCGATGAGC ATTTTTAACACACTCCATAGTCTCTTCCTGTGGTATCAGGTCTTTATTATTATTTTTTTTTTTTCTTTAT TTCCTGGGGCTGGGGGGTGGGCTGTCATGGGGGAACTGCCCTTTAAATTTTAAGTGACAGTACAGAAAAA TCA
Notes:	The probe template was PCR amplified from IMAGE:571882 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:571882 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10136 same embryo
	EMAGE:10139 same embryo
	EMAGE:10137 same embryo
	EMAGE:10138 same embryo
	EurExpress:euxassay_000015 same experiment
	MGI:4823306 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source