EMAGE mouse gene expression spatial database

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EMAGE:10139

Nsg2 neuron specific gene family member 2 ( MGI:1202070)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000024. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000024_01	euxassay_000024_02	euxassay_000024_03	euxassay_000024_04

euxassay_000024_05	euxassay_000024_06	euxassay_000024_07	euxassay_000024_08	euxassay_000024_09

euxassay_000024_10	euxassay_000024_11	euxassay_000024_12	euxassay_000024_13	euxassay_000024_14

euxassay_000024_15	euxassay_000024_16	euxassay_000024_17	euxassay_000024_18	euxassay_000024_19

euxassay_000024_20	euxassay_000024_21	euxassay_000024_22	euxassay_000024_23	euxassay_000024_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10139_wholemount_strong.wlz

10139_wholemount_moderate.wlz

10139_wholemount_weak.wlz

10139_wholemount_possible.wlz

10139_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10139_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

tail vertebral pre-cartilage condensation

strong

regional

strong expression: see section 16 17 moderate expression: see section 18

limb

weak

regional

weak expression: see section 03

visceral organ system

moderate

homogeneous

moderate expression: see section 15

central nervous system

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 24

peripheral nervous system

strong

homogeneous

strong expression: see section 08 10 11 12 13 15 16 17 18 19 20 21 22 24 moderate expression: see section 01 02 03 04 05 06 07 09

retina

strong

homogeneous

strong expression: see section 03 04 moderate expression: see section 01 02

nasal cavity olfactory epithelium

weak

homogeneous

weak expression: see section 14

vomeronasal organ epithelium

weak

homogeneous

weak expression: see section 14

axial skeleton

moderate

regional

moderate expression: see section 15 16 weak expression: see section 14 17

tail vertebral cartilage condensation

strong

regional

strong expression: see section 16 17 moderate expression: see section 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1755
Entity Detected:	Nsg2, neuron specific gene family member 2 ( MGI:1202070)
Sequence:	sense strand is shown >T1755 TGGCCTCGAGNCAGATTCGGCACGAGGCCGGGTTTTGCTTTTTCCCCAAAGCGCTAATGACAGCAGGAAT GACAATTGAATTCTAGTTAGTTGAAATCTGACAGCCGGGAAGAAACTTGAAGATTGGGCAGGCAGTGCTC ATTCCTAAAAGAAGCTGGAGAAGGTGACTGTTTGCTCACTGTGTTGTGTTGACAGAGTTGTCCAGACTTC CCAGCTAGGTTCTAGAAGCACCTGACAGGGAGGTGTGGCCAACATCCTTAGGCTTCTCACTGGCACTTTA GCAAGGGCCTCCTGCTTCTCGCGAGCATTCCAGGAAATCCCTTTTCTTGGAGGTGCTGGCCCCAGGTCCA AGAGAATGAGAACTGGCCAGGGGAATTGCCCATTTGGTCTGCATAGGCCGTTTAAGGGTATTTTTTTGTG GCTTGCTATATTGCTGAAATTATTGTACACAGCAGCTGGGTAATAGGAATAGTATATCTCAAACCATCCT GCCAGATACTCTCATCTGATTTCTCCTCCCTCCCCCCATCTTCCCATCACCTTGAGTCACCTGTAAATT
Notes:	The probe template was PCR amplified from IMAGE:482989 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:482989 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10135 same embryo
	EMAGE:10136 same embryo
	EMAGE:10137 same embryo
	EMAGE:10138 same embryo
	EurExpress:euxassay_000024 same experiment
	MGI:4826805 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source