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EMAGE:10148

Orc4 origin recognition complex, subunit 4 ( MGI:1347043)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10148 EMAGE:10148 EMAGE:10148 EMAGE:10148 EMAGE:10148
"Pseudo-wholemount" of euxassay_000026. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_000026_01 euxassay_000026_02 euxassay_000026_03 euxassay_000026_04
EMAGE:10148 EMAGE:10148 EMAGE:10148 EMAGE:10148 EMAGE:10148
euxassay_000026_05 euxassay_000026_06 euxassay_000026_07 euxassay_000026_08 euxassay_000026_09
EMAGE:10148 EMAGE:10148 EMAGE:10148 EMAGE:10148 EMAGE:10148
euxassay_000026_10 euxassay_000026_11 euxassay_000026_12 euxassay_000026_13 euxassay_000026_14
EMAGE:10148 EMAGE:10148 EMAGE:10148 EMAGE:10148 EMAGE:10148
euxassay_000026_15 euxassay_000026_16 euxassay_000026_17 euxassay_000026_18 euxassay_000026_19
EMAGE:10148 EMAGE:10148 EMAGE:10148 EMAGE:10148 EMAGE:10148
euxassay_000026_20 euxassay_000026_21 euxassay_000026_22 euxassay_000026_23 euxassay_000026_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10148Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10148_wholemount_strong.wlz
10148_wholemount_moderate.wlz
10148_wholemount_weak.wlz
10148_wholemount_possible.wlz
10148_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10148_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1783
Entity Detected:Orc4, origin recognition complex, subunit 4 ( MGI:1347043)
Sequence:sense strand is shown

>T1783
TGGCCTCGAGGCCAGATTCGGATCCATGGGCGGCCGGCTTTGCCCGATTTAGGCAGGCTTGACTGGGAGA
GGATTTGAATTAATCGAGATGAGCAGTCGTAAAACCAAGAGTAATGCACATGCAGAGTGTCTTTCACAGG
TACAAAGAATTTTACGTGAAAGATTTTGTCATCATAGTCCACATAGCAACCTCTTTGGAGTGCAAGTACA
ATACAAGCATTTGATTGAGCTACTCAAAAGAACTGCTATCTATGGAGAAAGCAATTCTGTACTCATTGTT
GGACCACGAGGATCAGGAAAGACCACATTATTAAATCATGCTTTGAAAGAACTCATGGAAATAGAAGTGA
GTGAAAATGTGATACAAGTTCACTTAAATGGACTCCTGCAGACAAATGAAAAAATTGCTCTGAAAGAGAT
CACAAGACAATTAAATCTAGACAATGTAGTTGAAGATAAAGTTTTTGGAAGCTTTGCTGAAAACCTTTCA
TTTCTTCTGGAAGCTTTGCAAAAAGGTGACCGGACTAGCAGTTGCCCAGTGATCTTCATACTGGATGAAT
TT
Notes:The probe template was PCR amplified from IMAGE:572595 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:572595 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:MGI:4826982 same experiment
 EurExpress:euxassay_000026 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS