EMAGE mouse gene expression spatial database

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EMAGE:10174

Kbtbd4 kelch repeat and BTB (POZ) domain containing 4 ( MGI:1914386)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000147. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000147_01	euxassay_000147_02	euxassay_000147_03	euxassay_000147_04

euxassay_000147_05	euxassay_000147_06	euxassay_000147_07	euxassay_000147_08	euxassay_000147_09

euxassay_000147_10	euxassay_000147_11	euxassay_000147_12	euxassay_000147_13	euxassay_000147_14

euxassay_000147_15	euxassay_000147_16	euxassay_000147_17	euxassay_000147_18	euxassay_000147_19

euxassay_000147_20	euxassay_000147_21	euxassay_000147_22	euxassay_000147_23	euxassay_000147_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10174_wholemount_strong.wlz

10174_wholemount_moderate.wlz

10174_wholemount_weak.wlz

10174_wholemount_possible.wlz

10174_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10174_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 18 weak expression: see section 19

diencephalon roof plate

strong

regional

strong expression: see section 12

telencephalon

strong

homogeneous

strong expression: see section 22

cerebral cortex

weak

regional

weak expression: see section 24

cerebral cortex mantle layer

strong

homogeneous

strong expression: see section 10 11 16 moderate expression: see section 15

cerebral cortex marginal layer

strong

homogeneous

strong expression: see section 17 22

cerebral cortex ventricular layer

strong

homogeneous

strong expression: see section 03 04 05 06 08 09 10 11 12 16 17 18 19 21 22 moderate expression: see section 01 02 15 20

olfactory lobe

moderate

regional

moderate expression: see section 13

olfactory cortex

strong

homogeneous

strong expression: see section 17 18

olfactory cortex marginal layer

strong

regional

strong expression: see section 16 17 18 moderate expression: see section 13

hindbrain

weak

regional

weak expression: see section 04 05 06 08

metencephalon rest of alar plate

strong

regional

strong expression: see section 11 weak expression: see section 10

metencephalon rest of alar plate ventricular layer

strong

homogeneous

strong expression: see section 11

midbrain

strong

regional

strong expression: see section 12 weak expression: see section 04 05 06 08

midbrain lateral wall

strong

regional

strong expression: see section 12 moderate expression: see section 14 weak expression: see section 10

midbrain mantle layer

strong

graded

strong expression: see section 11 moderate expression: see section 14 15

midbrain marginal layer

strong

graded

strong expression: see section 09 13 moderate expression: see section 15

midbrain ventricular layer

strong

regional

strong expression: see section 11 moderate expression: see section 14 weak expression: see section 10

glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 16 18 weak expression: see section 15

trigeminal v ganglion

strong

homogeneous

strong expression: see section 17 moderate expression: see section 05 18 19 20 weak expression: see section 06 08

vagus x ganglion

strong

regional

strong expression: see section 17 moderate expression: see section 05 16 weak expression: see section 06 08 15

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 17 moderate expression: see section 05 18 weak expression: see section 06 08

trigeminal v nerve maxillary division

weak

other

weak expression: see section 17 not examined expression: see section 09

trigeminal v nerve ophthalmic division

weak

homogeneous

weak expression: see section 09

spinal cord

strong

homogeneous

strong expression: see section 09 13 14 15 moderate expression: see section 10 12

dorsal root ganglion

strong

homogeneous

strong expression: see section 14 15 moderate expression: see section 08

retina

weak

regional

weak expression: see section 01 24

neural retina

weak

regional

weak expression: see section 01 02 24

metanephros

moderate

regional

moderate expression: see section 08 16 18 weak expression: see section 05 06

renal cortex

moderate

spotted

moderate expression: see section 16 18 weak expression: see section 17

nucleus pulposus

moderate

homogeneous

moderate expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1799
Entity Detected:	Kbtbd4, kelch repeat and BTB (POZ) domain containing 4 ( MGI:1914386)
Sequence:	sense strand is shown >T1799 GCTTTTGCCGAGTCACTAAGGACTAGCTTGAAGGAAATTGGGGAGAATGTGCACATTTACCTGATCGGAA AAGAGTCATCCCGCACGCACTCATTGGCTGTGTCCTTGCACTGTGCAGAAGATGACTCCATCAGTGTAAG TGGCCAAAACAGCTTATGCCACCAAATCACTGCAGCCTGCAAGCATGGTGGCGACCTGTATGTGGTAGGA GGGTCCATCCCACGACGCATGTGGAAATGTAACAATGCCACTGTTGACTGGGAGTGGTGTGCTCCTTTGC CCCGCGACCGCCTCCAGCACACCCTAGTATCTGTGCCTGGGAAAGATGCCATATATTCCCTGGGCGGCAA GACACTACAGGATACCCTCTCGAATGCAGTTATCTACTATCGGGTAGGTGATAACGTGTGGACAGAGACA ACTCAGTTAGAGGTGGCTGTGTCTGGGGCTGCTGGTGCCAACCTGAATGGAATCATCTACTTACTGGGGG GTGAGGAGAATGATCTAGACTTCTTTACCAA
Notes:	The probe template was PCR amplified from IMAGE:574723 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:574723 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10176 same embryo
	EMAGE:10175 same embryo
	EMAGE:10173 same embryo
	EurExpress:euxassay_000147 same experiment
	MGI:4825693 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source