EMAGE mouse gene expression spatial database

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EMAGE:1020

Pax3 paired box gene 3 ( MGI:97487)
TS17 (10.5 dpc)
in situ hybridisation

Data Images


Figure 7A of Conway et al., 1997 [PMID:9053326] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.	Figure 7C of Conway et al., 1997 [PMID:9053326] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Figure C is a higher magnification of the embryo shown in figure A. Image annotations: N - neural tube; A - common atrium; V - common ventricle; 1-6 - numbered develoing branchial arches; black arrow and white arrowheads - a stream of cells emerging from the occipital neural tube, transversing the developing branchial arches 3,4 and 6, and entering the aortic sac and aorto-pulmonary outflow tract; black arrowhead - forelimb bud; open arrows - truncal ridges of the outflow tract.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

1020_wholemount_strong_3D_1.wlz

1020_wholemount_moderate_3D_1.wlz

1020_wholemount_possible_3D_1.wlz

1020_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:1020_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

neural tube

detected

outflow tract

detected

regional

Streams of cells were detected passing though the developing third, fourth, and sixth branchial arches and entering the aorto-pulmonary outflow tract.

embryo

detected

regional

Streams of cells were detected passing though the developing third, fourth, and sixth branchial arches and entering the aorto-pulmonary outflow tract.

branchial arch

detected

regional

Streams of cells were detected passing though the developing third, fourth, and sixth branchial arches and entering the aorto-pulmonary outflow tract.

3rd branchial arch

detected

regional

Streams of cells were detected passing though the developing third, fourth, and sixth branchial arches and entering the aorto-pulmonary outflow tract.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:1101193
Entity Detected:	Pax3, paired box gene 3 ( MGI:97487)
Sequence:	sense strand is shown >MGI:1101193 AAGCTTACCGAGGCCCGAGTGCAGGTCTGGTTTAGCAACCGCCGTGCAAGATGGAGGAAACAAGCTGGAG CCAATCAACTGATGGCTTTCAACCATCTCATTCCGGGGGGATTCCCTCCCACCGCCATGCCGACCCTGCC AACATACCAGCTGTCGGAGACCTCTTACCAGCCCACGTCTATTCCACAAGCCGTGTCAGATCCCAGTAGC ACCGTCCACAGACCTCAGCCGCTTCCTCCGAGCACTGTACACCAAAGCACTATTCCTTCGAACGCAGACA GCAGCTCTGCCTACTGCCTCCCCAGCACCAGGCATGGATTTTCAAGCTATACAGACAGCTTTGTGCCTCC ATCGGGGCCCTCCAACCCCATGAACCCCACCATCGGCAATGGCCTTTCACCTCAGGTAATGGGACTTCTG ACCAACCACGGTGGGGTACCGCACCAGCCTCAGACCGACTATGCTCTCTCCCCTCTCACTGGGGGCCTGG AACCCACGACCACGGTGTCAGCCAGCTGCA
	nt 1157 - nt 1676 of NM_008781.2
Notes:	The Pax3 probe used in this study by Conway et al., 1997 [PMID:9053326] is described as "a 516 base pair HindIII-PstI fragment cloned from the 3' end (base pairs 1071 to 1590) of the Pax3 gene (Goulding et al., 1991 [PMID:2022185] )." Restriction analysis of GenBank seq NM_008781.2 with HindIII and PstI reveals a fragment of suitable size (ie. nt 1157-1676 as noted above).
Chemistry:	RNA
Strand:	antisense

Specimen

Organism:	mouse
Strain:	C3H/101 x CBA/Ca
Age:	10.5 dpc
Theiler Stage:	TS17
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Conway et al., 1997 [PMID:9053326] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:9053326] Conway SJ, Henderson DJ, Copp AJ 1997 Pax3 is required for cardiac neural crest migration in the mouse: evidence from the splotch (Sp2H) mutant. Development (124):505-14
	[ PMID:2022185] Goulding MD, Chalepakis G, Deutsch U, Erselius JR, Gruss P 1991 Pax-3, a novel murine DNA binding protein expressed during early neurogenesis. EMBO J (10):1135-47
Links:	MGI:1334345 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source