EMAGE mouse gene expression spatial database

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EMAGE:10207

Fhod3 formin homology 2 domain containing 3 ( MGI:1925847)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000020. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000020_01	euxassay_000020_02	euxassay_000020_03	euxassay_000020_04

euxassay_000020_05	euxassay_000020_06	euxassay_000020_07	euxassay_000020_08	euxassay_000020_09

euxassay_000020_10	euxassay_000020_11	euxassay_000020_12	euxassay_000020_13	euxassay_000020_14

euxassay_000020_15	euxassay_000020_16	euxassay_000020_17	euxassay_000020_18	euxassay_000020_19

euxassay_000020_20	euxassay_000020_21	euxassay_000020_22	euxassay_000020_23	euxassay_000020_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10207_wholemount_strong.wlz

10207_wholemount_moderate.wlz

10207_wholemount_weak.wlz

10207_wholemount_possible.wlz

10207_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10207_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

integumental system muscle

strong

single cell

strong expression: see section 01 02 03 04 05 07 08 09 11 12 14 17 18 19 22 23 moderate expression: see section 10 24

adrenal medulla

moderate

single cell

moderate expression: see section 06

foregut

strong

single cell

strong expression: see section 02 03 04 05 06

vibrissa dermal component

strong

single cell

strong expression: see section 22 23 24

thalamus mantle layer

weak

homogeneous

weak expression: see section 08

cerebral cortex mantle layer

strong

single cell

strong expression: see section 07 08 09 10 11 13 14 15 18 19 20 21 22 23 moderate expression: see section 05 06 24

cerebral cortex ventricular layer

moderate

single cell

moderate expression: see section 24 weak expression: see section 05 06 07 08 09 21

telencephalon ventricular layer

moderate

single cell

moderate expression: see section 02 03 04

medulla oblongata alar plate mantle layer

strong

single cell

strong expression: see section 15 moderate expression: see section 20

medulla oblongata basal plate mantle layer

strong

single cell

strong expression: see section 10 17 18 19 20 moderate expression: see section 09 13

pons mantle layer

strong

single cell

strong expression: see section 08 09 19

midbrain mantle layer

strong

single cell

strong expression: see section 13

facial vii ganglion

strong

single cell

strong expression: see section 07 09 20

glossopharyngeal ix ganglion

strong

single cell

strong expression: see section 20

trigeminal v ganglion

strong

single cell

strong expression: see section 04 05 06 07 09 10 19 20 21 22 23 24

vagus x ganglion

strong

single cell

strong expression: see section 20

inferior vagus x ganglion

strong

single cell

strong expression: see section 19

vestibulocochlear viii ganglion

strong

single cell

strong expression: see section 06 07

basal columns

moderate

single cell

moderate expression: see section 13 15

left ventricle cardiac muscle

moderate

homogeneous

moderate expression: see section 12 13 14 15 17 18

tongue muscle

strong

single cell

strong expression: see section 12 13 14 15 16

foregut-midgut junction duodenum

strong

single cell

strong expression: see section 13

hindgut

strong

single cell

strong expression: see section 14 15 16 19 20 21

midgut

strong

single cell

strong expression: see section 13 14 15 16 19 20 21

renal capsule

strong

homogeneous

strong expression: see section 08 09 10 11 18 19 20 21 moderate expression: see section 12

medullary renal tubule

strong

spotted

strong expression: see section 06

axial skeleton

strong

homogeneous

strong expression: see section 15

sacral vertebral cartilage condensation

strong

homogeneous

strong expression: see section 13

axial skeleton tail region

strong

homogeneous

strong expression: see section 13 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2129
Entity Detected:	Fhod3, formin homology 2 domain containing 3 ( MGI:1925847)
Sequence:	sense strand is shown >T2129 TGGCCTCGAGCCAGATTCGGACGAGGGCGTTCCAGGGCCAACCGCAAATCTTTGCGAAGGACCTTGAAGA GTGGCCTGACTCCAGAAGAAGCCAGAGCCCTGGGCCTGGTTGGCACATCTGAACTGCAACTGTGACTTTT GTAGGTTCTCAGAAGTGTGCTGAGCAGAGGACCAGATCTGGCTAGACCTGCCCCTTTCAGTAGAGGCAGG GAGACTTGACCTGAACATCTGATGGTTAGAACTGAGTCCTCATTTCTGAGTAACACACTGCTTGTCACCG TTGCGCATTGACATGGATCTCCAGAAATCTCCTGCATGTACTTTATCAAGTCAGCTGTGTTTCTCTTGTT TCCTTGATACCTGGTTTATTAGCTCCAAGTGTGACACCTTTTCTAGGAAGCAACAGTCCTTGTAAGGAAC AAATCACTTCTGTCACAGTTGTTAAGATAACATGGGGCAATGTGATTAGACTCAGAGACTGGGTCTCTAC AACAACCAAATGTCCAGATATAAATGCCAATGCTGTACACACATACAAGCACAGCTTGTTTAC
Notes:	The probe template was PCR amplified from IMAGE:870990 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:870990 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10208 same embryo
	EMAGE:10209 same embryo
	EurExpress:euxassay_000020 same experiment
	MGI:4824859 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source