EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10230

Trpv2 transient receptor potential cation channel, subfamily V, member 2 ( MGI:1341836)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000067. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000067_01	euxassay_000067_02	euxassay_000067_03	euxassay_000067_04

euxassay_000067_05	euxassay_000067_06	euxassay_000067_07	euxassay_000067_08	euxassay_000067_09

euxassay_000067_10	euxassay_000067_11	euxassay_000067_12	euxassay_000067_13	euxassay_000067_14

euxassay_000067_15	euxassay_000067_16	euxassay_000067_17	euxassay_000067_18	euxassay_000067_19

euxassay_000067_20	euxassay_000067_21	euxassay_000067_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10230_wholemount_strong.wlz

10230_wholemount_moderate.wlz

10230_wholemount_weak.wlz

10230_wholemount_possible.wlz

10230_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10230_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forebrain

moderate

regional

moderate expression: see section 08 09 10

telencephalon

moderate

regional

moderate expression: see section 11 12 13 14 15

medulla oblongata

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15

metencephalon

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15

metencephalon lateral wall

moderate

regional

moderate expression: see section 04

midbrain

moderate

regional

moderate expression: see section 08 11 12 13

facial vii ganglion

strong

homogeneous

strong expression: see section 18 moderate expression: see section 02 03 04 05

glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 16 moderate expression: see section 04 05

inferior glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 04 05

superior glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 04 05

trigeminal v ganglion

strong

homogeneous

strong expression: see section 15 16 18 19 20 moderate expression: see section 01 02 03 04 05 06 07

vagus x ganglion

strong

homogeneous

strong expression: see section 15 16 moderate expression: see section 06 07

vestibulocochlear viii ganglion

strong

homogeneous

strong expression: see section 16 moderate expression: see section 02 03 04

spinal cord

strong

regional

strong expression: see section 09 moderate expression: see section 08 10 11 12 13 14

dorsal root ganglion

strong

homogeneous

strong expression: see section 08 09 14 15 16 moderate expression: see section 06 07

retina

moderate

homogeneous

moderate expression: see section 01

testis

moderate

spotted

moderate expression: see section 01 02 03

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2156
Entity Detected:	Trpv2, transient receptor potential cation channel, subfamily V, member 2 ( MGI:1341836)
Sequence:	sense strand is shown >T2156 TGGCCTCGAGCCAGATTCGGACGAGGCTCCCCCTGTTAGTGTCATCCCTAGTGCTGGGCTGGCTGAACCT GCTTTATTATACACGTGGCTTTCAGCACACAGGCATCTACAGTGTCATGATCCAAAAGGTCATTCTGCGA GACCTGCTCCGCTTCCTGCTGGTCTACCTAGTCTTCCTTTTCGGCTTTGCTGTAGCCCTAGTAAGCTTGA GCCGGGAGGCCCGAAGTCCCAAAGCCCCTGAAGATAGCAACACCACTGTGACGGAAAAGCCCACGCTGGG TCAGGAGGAGGAGCCAGTCCCATATGGGGGCATTCTGGATGCCTCCCTAGAGCTGTTCAAGTTCACCATT GGTATGGGTGAGCTGGCTTTCCAGGAACAGCTGCGCTTTCGTGGGGTGGTGCTGCTGTTGCTGTTGGCCT ACGTCCTCCTCACCTACGTCCTACTGCTCAACATGCTCATTGCCCTCATGAGTGAGACTGTCAACAGCGT TGCCACTGACAGCTGGAGCATCTGGAAGTTGCAGAAAGCCATCTCTGTC
Notes:	The probe template was PCR amplified from IMAGE:875703 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:875703 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10228 same embryo
	EMAGE:10229 same embryo
	EMAGE:10227 same embryo
	EurExpress:euxassay_000067 same experiment
	MGI:4828942 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source