EMAGE mouse gene expression spatial database

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EMAGE:10264

Atp11a ATPase, class VI, type 11A ( MGI:1354735)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018926. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018926_01	euxassay_018926_02	euxassay_018926_03	euxassay_018926_04

euxassay_018926_05	euxassay_018926_06	euxassay_018926_07	euxassay_018926_08	euxassay_018926_09

euxassay_018926_10	euxassay_018926_11	euxassay_018926_12	euxassay_018926_13	euxassay_018926_14

euxassay_018926_15	euxassay_018926_16	euxassay_018926_17	euxassay_018926_18	euxassay_018926_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10264_wholemount_strong.wlz

10264_wholemount_moderate.wlz

10264_wholemount_weak.wlz

10264_wholemount_possible.wlz

10264_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10264_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 07 08 18 19

thyroid gland

strong

regional

strong expression: see section 12 moderate expression: see section 16

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19

metencephalon part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 18 19

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 08 19

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 09 18 19

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 weak expression: see section 09 10 11 12 15 16 17 18

midgut

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 moderate expression: see section 06 07

bladder

moderate

regional

moderate expression: see section 12 13 14

urethra of male

weak

regional

weak expression: see section 13 14

nucleus pulposus

weak

regional

weak expression: see section 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50128
Entity Detected:	Atp11a, ATPase, class VI, type 11A ( MGI:1354735)
Sequence:	sense strand is shown >T50128 TGCAAGGAACAGCGACTATGCAATACCCAAGTTTAAACACTTAAAGAAGATGCTTCTTGTTCATGGGCAT TTTTATTATATCAGGATATCTGAGCTTGTGCAATACTTCTTTTATAAGAACGTCTGCTTCATTTTCCCTC AGTTTTTGTACCAGTTCTTCTGCGGATTTTCACAGCAGACTTTGTACGATACTGCGTATCTGACTCTCTA CAACATCAGCTTCACTTCCCTCCCGATTCTCCTGTACAGCCTCATGGAGCAGCACGTGGGCATTGACGTG CTCAAGAGAGACCCGACCCTCTACAGAGACATTGCCAAGAATGCGCTGCTGCGCTGGCGGGTGTTCATTT ACTGGACGTTTCTCGGCGTTTTTGACGCTTTGGTATTTTTCTTTGGTGCTTATTTCATATTTGAGAACAC AACCGTGACCATCAACGGACAGATGTTTGGGAACTGGACCTTCGGGACGCTGGTGTTCACTGTGATGGTG CTCACTGTCACACTGAAGCTCGCACTGGACACGCACTACTGGACTTGGATCAACCACTTTGTCATCTGGG GGTCGCTGCTCTTCTACATTGCCTTCTCCCTGCTCTGGGGAGGGGTTATCTGGCCGTTCCTCAGTTACCA GAGGATGTACTATGTGTTCATATCCATGCTGTCCAGTGGGCCTGCCTGGCTGGGTATCATACTGCTTGTC ACGGTCGGCCTCCTCCCTGACGTCCTCAAGAAGGTCCTGTGTAGGCAGCTGTGGCCCACGGCAACGGAGA GAACTCAGAACATACAACATCAGGACAGCATTTCAGAATTCACCCCTCTGGCCTCTTTGCCGAGCTGGGG TGCTCAGGGCAGCAGACTCTTGGCTGCCCAGTGCAGCTCCCCCTCTGGGAGAGTTGTGTGCTCCAGGTGG GAGAGCGAAGAGTGTCCTGTGCTCCCTCTCCACCCAGGCCTTCCTCACAAGGCAAGGTATGGGTGCTGTA GGTCCAGCCTGGAGATGCCCACTTGATGGACAGACA
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TGCAAGGAACAGCGACTATG; Reverse Primer - name:unspecified, sequence:TGTCTGTCCATCAAGTGGGC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10263 same embryo
	EMAGE:10262 same embryo
	EurExpress:euxassay_018926 same experiment
	MGI:4823301 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source