EMAGE mouse gene expression spatial database

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EMAGE:10310

Tenc1 tensin like C1 domain-containing phosphatase ( MGI:2387586)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000565. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000565_01	euxassay_000565_02	euxassay_000565_03	euxassay_000565_04

euxassay_000565_05	euxassay_000565_06	euxassay_000565_07	euxassay_000565_08	euxassay_000565_09

euxassay_000565_10	euxassay_000565_11	euxassay_000565_12	euxassay_000565_13	euxassay_000565_14

euxassay_000565_15	euxassay_000565_16	euxassay_000565_17	euxassay_000565_18	euxassay_000565_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10310_wholemount_strong.wlz

10310_wholemount_moderate.wlz

10310_wholemount_weak.wlz

10310_wholemount_possible.wlz

10310_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10310_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 02 04 moderate expression: see section 05 06 07 17 weak expression: see section 18 19

hand mesenchyme

strong

regional

strong expression: see section 01 02 03

hindlimb digit 1 mesenchyme

strong

regional

strong expression: see section 01

hindlimb digit 2 mesenchyme

strong

regional

strong expression: see section 01

hindlimb digit 3 mesenchyme

strong

regional

strong expression: see section 01

hindlimb digit 4 mesenchyme

strong

regional

strong expression: see section 02

hindlimb digit 5 mesenchyme

strong

regional

strong expression: see section 03

otic capsule

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 16 17 18 19

viscerocranium

strong

regional

Expression in the turbinate bone.

meckel's cartilage

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

axial skeleton

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

cranium

strong

regional

strong expression: see section 01 02 03 04 05 06 17 18 19

pectoral girdle and thoracic body wall skeleton

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T469
Entity Detected:	Tenc1, tensin like C1 domain-containing phosphatase ( MGI:2387586)
Sequence:	sense strand is shown >T469 CTCGAGNCTGTTGGCCTACTGGTGACAGCCACAGAGGAAGGAGAGACAGGCCAGAAGAGCCATGAAGCCG AGGAAAGCAGAACCACACAGCTTCCGGGAGAAGGTTTTCCGGAAGAAAACTCCAGTGTGTGCAGTGTGTA AGGTGACCATCGATGGGACCGGTGTCTCCTGCCGAGTCTGCAAGGTGGCCACACACAGAAAATGTGAAGC AAAGGTGACTTCATCCTGTCAGGCCTTGCCCCCTGCGGAGCTGCGGAGAAGCACAGCCCCGGTCCGGCGC ATAGAACACCTGGGGTCCACCAAGTCTCTGAACCACTCAAAGCAACGCAGTACTCTACCCAGGAGCTTCA GCCTAGATCCGCTCATGGAGCGTCGCTGGGACTTGGACCTCACCTATGTAACGGAGCGGATCTTGGCCGC AGCTTTTCCTGCACGACCGGACGAGCAGCGACACCGAGGCCACTTGCGCGAGCTAGCCCATGTGCTTCAA TCCAAGCACAGAGACAAGTACCTGCTCTTCAACCTTTCAGAGAAACGGCATG
Notes:	The probe template was PCR amplified from IMAGE:1451210 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1451210 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10311 same embryo
	EMAGE:10309 same embryo
	EurExpress:euxassay_000565 same experiment
	MGI:4828660 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source