EMAGE mouse gene expression spatial database

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EMAGE:10407

Pygm muscle glycogen phosphorylase ( MGI:97830)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000529. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000529_01	euxassay_000529_02	euxassay_000529_03	euxassay_000529_04

euxassay_000529_05	euxassay_000529_06	euxassay_000529_07	euxassay_000529_08	euxassay_000529_09

euxassay_000529_10	euxassay_000529_11	euxassay_000529_12	euxassay_000529_13	euxassay_000529_14

euxassay_000529_15	euxassay_000529_16	euxassay_000529_17	euxassay_000529_18	euxassay_000529_19

euxassay_000529_20	euxassay_000529_21	euxassay_000529_22	euxassay_000529_23	euxassay_000529_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10407_wholemount_strong.wlz

10407_wholemount_moderate.wlz

10407_wholemount_weak.wlz

10407_wholemount_possible.wlz

10407_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10407_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

biceps brachii

strong

homogeneous

strong expression: see section 02 24

brachialis

strong

homogeneous

strong expression: see section 01 24

teres major

strong

homogeneous

strong expression: see section 01 24

triceps brachii

strong

homogeneous

strong expression: see section 01 24

diaphragm

strong

homogeneous

strong expression: see section 02 04 06 07 11 18 19 20 21 moderate expression: see section 09

pleuro-pericardial folds

strong

homogeneous

strong expression: see section 10

parietal pericardium

strong

homogeneous

strong expression: see section 10

external oblique muscle

strong

homogeneous

strong expression: see section 01 02 04 05 24

internal oblique muscle

strong

homogeneous

strong expression: see section 02 04 05 24

rectus abdominis

strong

homogeneous

strong expression: see section 01 02 04 05 24

transversus abdominis

strong

homogeneous

strong expression: see section 02 05 24

erector spinae

strong

homogeneous

strong expression: see section 20 21 22 moderate expression: see section 17

ilio-psoas

strong

homogeneous

strong expression: see section 20 22

intercostal muscle

strong

homogeneous

strong expression: see section 04

intercostal muscle external layer

strong

homogeneous

strong expression: see section 01

intercostal muscle internal layer

strong

homogeneous

strong expression: see section 01

psoas major

strong

homogeneous

strong expression: see section 11 13 16 17

psoas minor

strong

homogeneous

strong expression: see section 11 16 17

quadratus lumborum

strong

homogeneous

strong expression: see section 12 13 14 15 16 17 20

serratus ventralis

strong

homogeneous

strong expression: see section 01 02

masseter

strong

homogeneous

strong expression: see section 01 18 19 24

mylohyoid

strong

homogeneous

strong expression: see section 01 02 04 06 07 23 24

sterno-mastoid

strong

homogeneous

strong expression: see section 02 04 18

temporalis

strong

homogeneous

strong expression: see section 01 04 06 18

deltoid

strong

homogeneous

strong expression: see section 01 02 04 05 06 23 24

infraspinatus

strong

homogeneous

strong expression: see section 21

latissimus dorsi

strong

homogeneous

strong expression: see section 04 06 23

pectoralis major

strong

homogeneous

strong expression: see section 01 05 06 07 10 16 18 19 20 21 22 moderate expression: see section 09

pectoralis minor

strong

homogeneous

strong expression: see section 05 06 10 12 18 19 20 21 22 moderate expression: see section 09

subscapularis

strong

homogeneous

strong expression: see section 01 05 23

supraspinatus

strong

homogeneous

strong expression: see section 01 06

trapezius

strong

homogeneous

strong expression: see section 01 04 05 06 20 21 23 moderate expression: see section 09

pelvic girdle muscle

strong

homogeneous

strong expression: see section 04

gluteus maximus

strong

homogeneous

strong expression: see section 02 05 06 07 10 20 21 22 23 moderate expression: see section 09

extrinsic ocular muscle

strong

homogeneous

strong expression: see section 01 02 04 05 06

heart

strong

homogeneous

strong expression: see section 11 13 14 16 moderate expression: see section 15 17 weak expression: see section 08 09 10

endocardial tissue

weak

homogeneous

weak expression: see section 10

heart ventricle

strong

homogeneous

strong expression: see section 16 17 moderate expression: see section 15

tongue

strong

homogeneous

strong expression: see section 14

tongue extrinsic muscle

strong

homogeneous

strong expression: see section 11 12 13 14 15 17 moderate expression: see section 10

genioglossus

moderate

homogeneous

moderate expression: see section 08 09 10

hyoglossus

moderate

homogeneous

moderate expression: see section 08 09 10

palatoglossus

moderate

homogeneous

moderate expression: see section 10

styloglossus

moderate

homogeneous

moderate expression: see section 10

tongue intrinsic muscle

strong

homogeneous

strong expression: see section 11 12 13 14 15 moderate expression: see section 10

tongue intrinsic muscle transverse component

moderate

homogeneous

moderate expression: see section 10

tongue intrinsic muscle vertical component

moderate

homogeneous

moderate expression: see section 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3608
Entity Detected:	Pygm, muscle glycogen phosphorylase ( MGI:97830)
Sequence:	sense strand is shown >T3608 GCCTGGGCCTCAGGTCTTCCCAGCTTTTCTCTGGGAAGCTGGGGACCCCTTTGTGGGGAGCTGATTACAG GTGCGCTCTGGCAGGTACAATGCCCAGGAGTACTACGACCGAATTCCTGAGCTTCGGCAGATCATCGAAC AGCTAAGCAGCGGCTTCTTCTCCCCCAAGCAGCCTGACCTGTTCAAGGACATTGTCAATATGCTCATGCA CCATGACCGGTTTAAAGTCTTTGCAGATTATGAGGAATACATTAAATGCCAGGACAAAGTCAGTGAGTTG TACAAGAACCCAAGAGAGTGGACACGGATGGTGATCAGGAACATAGCCACCTCTGGCAAGTTTTCCAGTG ACCGCACCATTGCCCAGTATGCCCGGGAGATCTGGGGTGTTGAACCTTCTCGCCAGCGCCTGCCAGCCCC AGATGAGAAGATCTGAGCTTCCACATCAGACCCCATACCCGCCCTTGAGTCTGTTTGCAATTCCTGGGAT CAGGCCCACTCCAGGAGGTGGGCCTCTGGAATTACATCTCTAC
Notes:	The probe template was PCR amplified from IMAGE:3167783 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3167783 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10403 same embryo
	EMAGE:10404 same embryo
	EMAGE:10406 same embryo
	EMAGE:10405 same embryo
	EurExpress:euxassay_000529 same experiment
	MGI:4827552 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source