EMAGE mouse gene expression spatial database

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EMAGE:10450

Krt8 keratin 8 ( MGI:96705)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000582. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000582_01	euxassay_000582_02	euxassay_000582_03	euxassay_000582_04

euxassay_000582_05	euxassay_000582_06	euxassay_000582_07	euxassay_000582_08	euxassay_000582_09

euxassay_000582_10	euxassay_000582_11	euxassay_000582_12	euxassay_000582_13	euxassay_000582_14

euxassay_000582_15	euxassay_000582_16	euxassay_000582_17	euxassay_000582_18	euxassay_000582_19

euxassay_000582_20	euxassay_000582_21	euxassay_000582_22	euxassay_000582_23	euxassay_000582_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10450_wholemount_strong.wlz

10450_wholemount_moderate.wlz

10450_wholemount_weak.wlz

10450_wholemount_possible.wlz

10450_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10450_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

thymus primordium

strong

homogeneous

strong expression: see section 13 14 15 16 17

submandibular gland primordium

strong

regional

strong expression: see section 08 09 10 11 18 19 20 21

foregut-midgut junction

strong

regional

strong expression: see section 13 14 15 16 17 18 19 20 21 22

pancreas

moderate

regional

moderate expression: see section 10 11 12 13

pituitary gland

weak

regional

weak expression: see section 12 13 14 15 16 17

vibrissa

weak

regional

weak expression: see section 05 06 07 08 24

lateral ventricle choroid plexus

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

4th ventricle

weak

regional

weak expression: see section 04 05 06 07 08 10 11 12 13 14 15 16 17 18 19 20 21

ear

strong

regional

strong expression: see section 10

cochlea

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 18 19 20 21 24

pharyngo-tympanic tube

strong

regional

strong expression: see section 10 moderate expression: see section 01 04 05 06 07 08 09 20 21 22 23 24 weak expression: see section 02 03

tubotympanic recess

moderate

regional

moderate expression: see section 03 05 06 07 08 09 10 19 20 21 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 19 20 21

anal canal

strong

regional

strong expression: see section 15 16

esophagus

strong

regional

strong expression: see section 13 14 15

stomach

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12

hindgut

strong

regional

strong expression: see section 15 16 17

midgut

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21

oral epithelium

moderate

regional

moderate expression: see section 07 08 10 11 12 13 14 15 16 17 18 19 20 weak expression: see section 09 21 22 23 24

liver lobe

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

bladder

moderate

regional

moderate expression: see section 15 16

kidney calyx

moderate

regional

moderate expression: see section 06 07 08 09 10 12 18 21 22

kidney pelvis

moderate

regional

moderate expression: see section 11 19 20

male reproductive system

strong

regional

strong expression: see section 15 16

urethra of male

strong

regional

strong expression: see section 15 16 weak expression: see section 17

laryngeal cartilage

strong

regional

strong expression: see section 13 14 15

lung

moderate

spotted

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T810
Entity Detected:	Krt8, keratin 8 ( MGI:96705)
Sequence:	sense strand is shown >T810 TCTCGAGNCTGTTGGCCTACTGGACCGTCTAGAAGCAGCTGCTTAGCTCGCTCTCGAACCTCCGTCTTCA GCTCACTGCCTTCGCTCCAGACTTCACCATGTCCATCAGGGTGACTCAGAAATCCTACAAGATGTCCACC TCCGGTCCCCGGGCCTTCAGCAGCCGCTCGTTCACGAGTGGACCCGGTGCCCGCATCAGCTCTTCCAGCT TTTCCCGGGTGGGCAGCAGCAGCAGCAGCTTCCGGGGAAGCATGGGCACCGGCGTGGGTCTGGGCGGCTT TGGCGGGGCTGGTGTCGGGGGCATCACAGCCGTCACGGTGAACCAGAGCCTGTTGAGCCCCTTGAAGCTG GAGGTGGACCCCAACATCCAGGCTGTGCGCACTCAGGAGAAGGAGCAGATTAAATCCCTGAACAACAAGT TCGCCTCCTTCATTGACAAGGTGCGCTTCCTGGAGCAGCAGAACAAGATGCTGGAGACCAAGTGGAGCCT GTTGCAGCAGCAGAAGACGTCGAGGAGCAACATGGACAACATGTTTGAGAGCTA
Notes:	The probe template was PCR amplified from IMAGE:1921806 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1921806 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10448 same embryo
	EMAGE:10449 same embryo
	EMAGE:10451 same embryo
	EurExpress:euxassay_000582 same experiment
	MGI:4825848 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source