EMAGE mouse gene expression spatial database

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EMAGE:10546

Nav1 neuron navigator 1 ( MGI:2183683)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000852. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000852_01	euxassay_000852_02	euxassay_000852_03	euxassay_000852_04

euxassay_000852_05	euxassay_000852_06	euxassay_000852_07	euxassay_000852_08	euxassay_000852_09

euxassay_000852_10	euxassay_000852_11	euxassay_000852_12	euxassay_000852_13	euxassay_000852_14

euxassay_000852_15	euxassay_000852_16	euxassay_000852_17	euxassay_000852_18	euxassay_000852_19

euxassay_000852_20	euxassay_000852_21	euxassay_000852_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10546_wholemount_strong.wlz

10546_wholemount_moderate.wlz

10546_wholemount_weak.wlz

10546_wholemount_possible.wlz

10546_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10546_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon mantle layer

moderate

homogeneous

moderate expression: see section 02 not examined expression: see section 01 03 04 05 06

not examined

homogeneous

not examined expression: see section 03 04 05 06

not examined

homogeneous

not examined expression: see section 05

not examined

homogeneous

not examined expression: see section 06

not examined

homogeneous

not examined expression: see section 06

not examined

homogeneous

not examined expression: see section 05 06

facial vii ganglion

moderate

homogeneous

moderate expression: see section 04 05 06 18 19

inferior glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 06 17

superior glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 17

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 03 04 05 06 08 09 16 17 18 19 20 21

vagus x ganglion

moderate

homogeneous

moderate expression: see section 08 15 16

vestibulocochlear viii ganglion vestibular component

moderate

homogeneous

moderate expression: see section 06 17 18

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 14 15 16

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17

not examined

regional

Expression in the turbinate bone.

meckel's cartilage

moderate

regional

moderate expression: see section 08 09 15 16 17 18 19

upper jaw molar

moderate

regional

moderate expression: see section 08 09 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2366
Entity Detected:	Nav1, neuron navigator 1 ( MGI:2183683)
Sequence:	sense strand is shown >T2366 TGGCCTCGAGCCAGAATTCGGATCCTTGGGTTCTGGACAGAAAGCTGCTTGGGAAGACCCGGTGGAATGG GTCCGAGACACTCTTCCCTGGCCGTCGGCCCAACAAGACCAATCAAAGCTCTACCACCTGCCCCCGCCTT CTGTGGGCCCCCACAGCACTGCCTCACCCCCGGAGGACAGGACAGTCAAAGACAGCACTCCAAACTCCCT CGACTCAGATCCCCTGATGGCCATGCTGCTGAAACTCCAAGAAGCTGCCAACTACATTGAGTCACCAGAT CGAGAGACTATCCTGGACCCCAACCTCCAGGCGACACTCTGAGGGCCCGGCAGAAGGCTGGCTTCAGCGT CATTAGCTCTCCTCTGCCCTCTTCCTTCATAGCTCTGGCTCACCAGCCTCGCCAAGAGAACAGGAGGGAA GAAGAGGGCAGGAGGAGGGATGGGTTCTCGGTGCTGAACCTTTGAGAACTTCCTACTAGGAATTGGAGGG GGTGGAGTTTGAGAAATCCGTGTCCCTTAAATACATTTGCTGGC
Notes:	The probe template was PCR amplified from IMAGE:1180081 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1180081 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10544 same embryo
	EMAGE:10543 same embryo
	EMAGE:10545 same embryo
	EMAGE:10542 same embryo
	EurExpress:euxassay_000852 same experiment
	MGI:4826604 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source