EMAGE mouse gene expression spatial database

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EMAGE:10627

Enpp2 ectonucleotide pyrophosphatase/phosphodiesterase 2 ( MGI:1321390)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000979. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000979_01	euxassay_000979_02	euxassay_000979_03	euxassay_000979_04

euxassay_000979_05	euxassay_000979_06	euxassay_000979_07	euxassay_000979_08	euxassay_000979_09

euxassay_000979_10	euxassay_000979_11	euxassay_000979_12	euxassay_000979_13	euxassay_000979_14

euxassay_000979_15	euxassay_000979_16	euxassay_000979_17	euxassay_000979_18	euxassay_000979_19

euxassay_000979_20	euxassay_000979_21	euxassay_000979_22	euxassay_000979_23	euxassay_000979_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10627_wholemount_strong.wlz

10627_wholemount_moderate.wlz

10627_wholemount_weak.wlz

10627_wholemount_possible.wlz

10627_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10627_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

integumental system muscle

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

moderate

regional

moderate expression: see section 05 06 07 08 09 10 23 24

lateral ventricle choroid plexus

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

4th ventricle

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate ventricular layer

moderate

homogeneous

moderate expression: see section 14

pons ventricular layer

moderate

regional

moderate expression: see section 13 14 weak expression: see section 09 10 11 12 15 16 17 18 19

midbrain ventricular layer

moderate

regional

moderate expression: see section 13 14 weak expression: see section 10 11 12 15 16 17

ventral grey horn

moderate

regional

moderate expression: see section 14 15

lower jaw incisor

moderate

regional

moderate expression: see section 13 14 15 17 18

lower jaw molar

moderate

regional

moderate expression: see section 13 14 15 17 18

oral epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 20 21 22

upper jaw incisor

moderate

regional

moderate expression: see section 13 14 15 18

upper jaw molar

moderate

regional

moderate expression: see section 13 14 15 18

bladder

moderate

regional

moderate expression: see section 14 15 16 18

kidney calyx

moderate

homogeneous

moderate expression: see section 10 11 12 20 22 23

laryngeal cartilage

moderate

regional

moderate expression: see section 13 14

trachea cartilaginous ring

moderate

regional

moderate expression: see section 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2360
Entity Detected:	Enpp2, ectonucleotide pyrophosphatase/phosphodiesterase 2 ( MGI:1321390)
Sequence:	sense strand is shown >T2360 GNCCTCGAGGCCAGATTCGGCACGAGGAACTGTTTAGCCTATAAAAATGATAAACAGATGTCCTATGGAT TCCTTTTTCCTCCCTATCTGAGCTCTTCCCCAGAAGCGAAATATGATGCATTCCTTGTAACCAACATGGT TCCAATGTACCCTGCCTTCAAACGTGTTTGGACTTATTTCCAAAGGGTCTTGGTGAAGAAATATGCGTCA GAAAGGAATGGGGTCAACGTAATAAGTGGACCGATCTTTGACTACAATTACAATGGCTTACGTGACATTG AGGATGAAATTAAACAGTATGTGGAAGGCAGCTCTATTCCTGTCCCTACCCACTACTACAGCATCATCAC CAGCTGCCTGGACTTCACTCAGCCTGCAGACAAGTGTGATGGTCCTCTCTCTGTGTCTTCTTTCATCCTT CCTCACCGACCTGACAATGATGAGAGCTGTAATAGTTCCGAGGATGAGTCGAAGTGGGTAGAGGAACTCA TGAAGATGCACACAGCTCGGGTGAGGGACATCGAGCATCTCACCGGTTTGGATTTCTACCGGAAGACTAG CCGTAGCTATTCG
Notes:	The probe template was PCR amplified from IMAGE:1179261 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1179261 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10628 same embryo
	EMAGE:10629 same embryo
	EurExpress:euxassay_000979 same experiment
	MGI:4824561 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source