EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10633

Cttnbp2nl CTTNBP2 N-terminal like ( MGI:1933137)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003204. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003204_01	euxassay_003204_02	euxassay_003204_03	euxassay_003204_04

euxassay_003204_05	euxassay_003204_06	euxassay_003204_07	euxassay_003204_08	euxassay_003204_09

euxassay_003204_10	euxassay_003204_11	euxassay_003204_12	euxassay_003204_13	euxassay_003204_14

euxassay_003204_15	euxassay_003204_16	euxassay_003204_17	euxassay_003204_18	euxassay_003204_19

euxassay_003204_20	euxassay_003204_21	euxassay_003204_22	euxassay_003204_23	euxassay_003204_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10633_wholemount_strong.wlz

10633_wholemount_moderate.wlz

10633_wholemount_weak.wlz

10633_wholemount_possible.wlz

10633_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10633_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 22 23 24

submandibular gland primordium

strong

regional

strong expression: see section 09 10 12 14 20 21 22 23 24 moderate expression: see section 11 13

vibrissa

moderate

regional

moderate expression: see section 10 weak expression: see section 09 11 24

stomach

moderate

regional

moderate expression: see section 03 04 05 06 08 09 10 11

hindgut

strong

regional

strong expression: see section 15 16 17 18 moderate expression: see section 13 14

rectum

moderate

regional

moderate expression: see section 13

midgut

strong

regional

strong expression: see section 15 16 17 18 19 20 moderate expression: see section 06 07 08 09 10 11 12 13 14

oral epithelium

moderate

regional

moderate expression: see section 08 10 11 22 23 24

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

bladder

weak

regional

weak expression: see section 13 14 15

kidney calyx

moderate

regional

moderate expression: see section 07 08 09 10 19 20 21 22

urethra of male

weak

regional

weak expression: see section 13 14

orbito-sphenoid

moderate

regional

moderate expression: see section 04 05 06 07 08 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6184
Entity Detected:	Cttnbp2nl, CTTNBP2 N-terminal like ( MGI:1933137)
Sequence:	sense strand is shown >T6184 GGAGCGCCGAGGCCAGTGTTGGACACTTTCCCACCATGCCGACAACAGTTTAAATATCTGTCAGCTTTCT AAAAGTTTAACTGAAGGAAACCTCTAAGACTTCGTACCTGAGGACCAAAGTGCAGCCACCAATTATCAGA CTTTCAGGATGAATCTGGAAAAACTCAGCAAGCCTGAACTCCTGACACTGTTCAGTATCCTTGAAGGAGA ACTTGAAGCAAGAGACCTTGTTATAGAAGCTTTAAAGGCCCAACACAGAGATACTTTCATTGAAGAACGC TATGGAAAATATAACATCAGTGACCCTCTAATGGCTCTTCAGAGAGATTTTGAAACACTGAAGGAGAAAA ATGATAGTGAGAAGCAGCCAGTGTGCACAAACCCCCTCTCTGTCCTCAAGGCGGTGATGAAGCAGTGCAA GAACATGCAGGAGCGCATGCTGTCCCAGCTGGCCGCAGCCGAGAGCAGGCACCGCAAGGTGATCCTCGAC CTTGAAGAAGAAAGACAGAGGCATGCACAGGACACTGCTGAAGGAGATGATGTCACCTACATGCTGGAGA AGGAGAGGGAGAGACTGACGCAGCAGTTGGAATTTGAGAAGTCCCAAGTGAAAAAGTTTGAAAAAGAACA AAAGAAGCTGTCCAGTCAGTTGGAGGAGGAGCGCACCCGCCACAAGCAGCTCTCTTCCATGCTGGTGCTC GAGTGCAGGAAGGCCACTAGCAAGGCAGCCGAGGAAGGGCAGAAGGCCGGAGAGCTGAGCCTGAAACTGG ACAAGGAGAAGAGCCGGGCGAGCAAGCTAGAGGAAGAGTTGGCAGCCGAGAGGAAGCGGGGCCTGCAGAC GGAGGCCCAGGTGGAGAAGCAGCTGTCTGAGTTCGATATCGAAAGGGAGCAGCTGAGAGCCAAACTGAAC CGAGAGGAGAACCGGACCCGTGCCCTGAAGGAGGAGGTGGAGAGCCTGAAGAAGCTTGTGAAAGACCTAG AGGCAGCCCAGCAGCACCGCAGCACCAGTGAGCAGGGCAGGGAGCCAGTGACCATGTCCAGAGGCACAGC CACGGAGCCTCCCATGCGAGTGTCTGCGTTTTGTCAAACAGAGAGTGTTCAGACAGAAAGAAGCCACGGG AGTGTCATAACCAAGCTGACAGACACTGGTCTTCCAGGTCCCACCACGGCTGCTTACTCATATGCTAAAG CTAATGGCCACTGTGACCCAGAGATACAGACTACCAGGGAGTTGACCTCAGACAGCAGCACAGAAAACCA AGGGCCTCCACGGGAGAAATCTGCAGTAGCGGCTCAGGAGAAACCAGTGGAGAATGGTGGGTGTCCTGTA GGAACTGAGACTCCAGTCACGATGCCTAGTCACCTCCCTTCCAGTGGCAGCTCCCTGTCTCCCAGCAGCA CAGCCTCTTCCTCTCTGACATCATCTCCTTGCTCCTCCCCAGTTCTAACCAAGCGTCTCTTGGGGTCAGC AGCCAGCAGCCCGGGCTACCAGTCTTCCTACCAAGTCGGGATCAACCAGCGCTTCCATGCCGCTCGGCAC AAATTTCAGTCCCAGGCGGATCAGGATCAGCAAGCCAGCGGTCTCCAGAGCCCTCCATCCAGGGACCTGT CCCCTACCCTGTTAGACAACTCTGCTGCCAAGCAGCTGGCCCGAAACACAGTCACTCAGGTGCTCTCCAG ATTCACTAACCAAGGGCCAATTAAGCCCGTCTCTCCCAACAGTTCTCCCTTTGGGACAGACTACCGAAAT CTGGCCAGCACTGCCAGCCCAAGAGGTGACACCAGCCATTCACCCACTCCAGGGAAAGTGTCCAGCCCTC TGAGCCCCCTCTCTCCAGGAATCAAGTCCCCAACCATCCCCAGAGCTGAGAGAGGAAACCCTCCGCCAAT CCCGCCCAAAAAGCCTGGCCTCACCCCTTCGCAGTCTGCCACCACTCCAGTAACCAAGACTCATTCCCAG GCATCCTCCCTGGCCGCCACAGAAGACCTCGCCAGCAGCTGCTCCCCCAGTGCCGTGGTGGCCAATGGCA AAGATGTTGAGATACTTTTGCCTACCAGCAGCTAGTCCCTAGGGCTGAGTCTCCACATTTGACATTCCAT CAGACTCGGGCCAAGAGCTCAGCGTTAAACCCTGGAGTCAAATCATGTTATTTATTTGATAGTAGCTACA GCCATCTGTATAATACATTCAGTGTATTTACCTTTTTGTATTTTTTTAAGTAGAAATTGAATACTTTGGA TTTTTATGACTCACCTCTTTGTACTAAGCTAGAAGGGGACCTCATAGACATAGCGCATCTCAAGCTCTGC ACTTGCCAGCGTGCTGCGACGAGGCGGCTGACTGGGGACCAGGCGGTGCTACTGCCAGTCGGGGAGCTAG AATTCATTACTCAGCACTCATTTCAAATTATGCAGAAGCGGTTCGTGCAGATTTTTATTCCAGATGAACA TGTTTTAAAAGTGCCTGAAATCGCCTGCCACGGGAATGTGATTCTGCGGCAGAAACAAAAGACAGAGCAT TTCTTGCAGCAAATGAGATCCTCTGTGAATCTGAATGTCAAAAAACCAACACTGCTTTTAATCCTCTCTG ACTGTTTAATGCCAGCTTTGTGTTCTGAACCGAGGTTTGCATAAGTAGAGTGGAAATCCTTCTGAAGGGC GGGAACTCTGTCAGGCTGAGCTCTGTAAAGCCCTCAAGAAACCCTCCAGAACACTTAGCAGTTGGGGTGC TGATTTTCTTGTGCTTTTGAAAACTTAAGTCATTCTCCGTTTCCTGCATCACTTCTTGAGTAGTGAAGTC ACGTCTCCATTCAAATCCACCCTCAAGCATCGGCCATGGGAACTTCTGATTCTGGTTGCTTTTACTTGAA TAGGAAATGACATAATGAACTCTTTATTCTGTGATCAAAAAGCAATAACTTAAAAGTCAGTCTTTGTAAT ACTGTAAGTCAGAATTATACAGATTTTACCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3498078 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3498078 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10636 same embryo
	EMAGE:10634 same embryo
	EMAGE:10635 same embryo
	EurExpress:euxassay_003204 same experiment
	MGI:4824119 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source