EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10659

Mybl2 myeloblastosis oncogene-like 2 ( MGI:101785)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000945. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000945_01	euxassay_000945_02	euxassay_000945_03	euxassay_000945_04

euxassay_000945_05	euxassay_000945_06	euxassay_000945_07	euxassay_000945_08	euxassay_000945_09

euxassay_000945_10	euxassay_000945_11	euxassay_000945_12	euxassay_000945_13	euxassay_000945_14

euxassay_000945_15	euxassay_000945_16	euxassay_000945_17	euxassay_000945_18	euxassay_000945_19

euxassay_000945_20	euxassay_000945_21	euxassay_000945_22	euxassay_000945_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10659_wholemount_strong.wlz

10659_wholemount_moderate.wlz

10659_wholemount_weak.wlz

10659_wholemount_possible.wlz

10659_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10659_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 12 16 weak expression: see section 13 14 15

not examined

homogeneous

not examined expression: see section 16

diencephalon lateral wall ventricular layer

moderate

homogeneous

moderate expression: see section 11 12 13

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 09 moderate expression: see section 10 14 15 16

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 05 08 09 moderate expression: see section 01 02 03 04 06 07 10 11 12 13 14 15 16 17 18 19 20 21 22

cerebellum intraventricular portion ventricular layer

moderate

homogeneous

moderate expression: see section 03 04 05 20 21 22 weak expression: see section 06 07 08 09 10 16 17 18 19

midbrain ventricular layer

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15 16 weak expression: see section 07

liver lobe

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

skeleton

weak

regional

weak expression: see section 01 02 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T421
Entity Detected:	Mybl2, myeloblastosis oncogene-like 2 ( MGI:101785)
Sequence:	sense strand is shown >T421 GGACAATGCTGTGAAGAATCACTGGAACTCCACTATCTAAGGAAAGTCGACACGGGAGGTTTCCCAGNCG AGTCCAGNGACTGCAAGCCTGTCTACTTGCTCCTGGAGCTGGAGGACAAGGAACAGCACCAGGGTGTCCA GCCGGTGGACGGCCAGGGAAGTCTCGTGAGCAGCTGGCCGCTGGTGCCCTCTATTGTGAAGGAGGAGAGC AGCGAGGAGGAGATTGCCATAGCTGCTACTTCTGCTAAAGAACTCGGACATGAGCCTGTCCCTGCTGATC TGGGAGAAGTGCGCACCCCAGAGCCCCCAGAATCTCTCAAGCGTGAATACCAGGAGTTCTCCTCCCCGGA AACGAGCCTGCCCTACNAGTGGGTGGTAGAGGCGGCAAACCTCCTCATCCCGGCTGTGGGGTCCAGCCTC TCTGAAGCTCTGGACTTGATTGAGTCGGACCCTGATGCTTGGTGCGACCTGAGTAAATTTGACCTTCCTG AAGAAC
Notes:	The probe template was PCR amplified from IMAGE:3157976 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3157976 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10658 same embryo
	EMAGE:10657 same embryo
	EMAGE:10656 same embryo
	EMAGE:10655 same embryo
	EMAGE:10654 same embryo
	EurExpress:euxassay_000945 same experiment
	MGI:4826528 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source