EMAGE mouse gene expression spatial database

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EMAGE:10674

Csdc2 cold shock domain containing C2, RNA binding ( MGI:2146027)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000899. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000899_01	euxassay_000899_02	euxassay_000899_03	euxassay_000899_04

euxassay_000899_05	euxassay_000899_06	euxassay_000899_07	euxassay_000899_08	euxassay_000899_09

euxassay_000899_10	euxassay_000899_11	euxassay_000899_12	euxassay_000899_13	euxassay_000899_14

euxassay_000899_15	euxassay_000899_16	euxassay_000899_17	euxassay_000899_18	euxassay_000899_19

euxassay_000899_20	euxassay_000899_21	euxassay_000899_22	euxassay_000899_23	euxassay_000899_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10674_wholemount_strong.wlz

10674_wholemount_moderate.wlz

10674_wholemount_weak.wlz

10674_wholemount_possible.wlz

10674_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10674_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

strong

regional

strong expression: see section 11 12 moderate expression: see section 10 14 15

hypothalamus ventricular layer

strong

regional

strong expression: see section 11 12 moderate expression: see section 13

cerebral cortex

strong

homogeneous

strong expression: see section 05 06 07 08 09 10 11 18 19 20 21 moderate expression: see section 03 04

corpus striatum

strong

regional

strong expression: see section 02 03 04 05 06 07 08 18 19 20 21

olfactory cortex mantle layer

moderate

homogeneous

moderate expression: see section 10 11 12 15 16 18

not examined

homogeneous

not examined expression: see section 10 11 12

facial vii ganglion

strong

homogeneous

strong expression: see section 03 04 20

glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 03

inferior glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 06 07 moderate expression: see section 18

superior glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 06 07 18

trigeminal v ganglion

strong

homogeneous

strong expression: see section 02 04 05 06 07 08 18 19 20 moderate expression: see section 17 21 22

inferior vagus x ganglion

strong

homogeneous

strong expression: see section 08 moderate expression: see section 17

superior vagus x ganglion

strong

homogeneous

strong expression: see section 08 moderate expression: see section 17

vestibulocochlear viii ganglion vestibular component

strong

homogeneous

strong expression: see section 04 05 19 moderate expression: see section 20

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2174
Entity Detected:	Csdc2, cold shock domain containing C2, RNA binding ( MGI:2146027)
Sequence:	sense strand is shown >T2174 AATTCGGATCCACGTGCCCTGAGAAATGGCTGCAAGCAGGAGGGAACTTTAGCACTCCTGTTATGTAGGC CAGTCCTCTCTCCTCAGCTGTCAGATGATCTTTGAACCCCACAGAGCTGAGGAAGCCAAGCTCACTCACT CCCTGAGGTGAGGCGGGGATAGGGTTTCCTTCCAGCTTGCTCTTCTACCCGGGATGCTCAAACCTCACGA ATGCTCTGGCCCATTGCTGGCCATGACAGCAGCTGAGGATGCAGCAATGGACTGACATGTCACCTCAGGC AAGGCCAGGTCCAGGCACCCAAAGGATCGGTCCTCAGCCAGGGAGACCACTGGCTTTGCCCCTCCCACCC CCCATGAGATCCTCTTCCCAGAGCTATGCAATGGCTCTCATAAGCCCCTGACAGGTCAGCAGCTGAGGTG GCTCTTATCTCTCTTGTCCCCAAGTGTGACTGTCCCTCTTCCCTGGAGTCCCTTTGGAGCTGTGTCCCTT CTAGAGAGCCAGTTACTTAACCAGAGGATAAGTGAGGTCACA
Notes:	The probe template was PCR amplified from IMAGE:889232 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:889232 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10670 same embryo
	EMAGE:10672 same embryo
	EMAGE:10669 same embryo
	EMAGE:10671 same embryo
	EMAGE:10673 same embryo
	EurExpress:euxassay_000899 same experiment
	MGI:4824076 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source