EMAGE mouse gene expression spatial database

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EMAGE:10736

Senp5 SUMO/sentrin specific peptidase 5 ( MGI:2443596)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001053. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001053_01	euxassay_001053_02	euxassay_001053_03	euxassay_001053_04

euxassay_001053_05	euxassay_001053_06	euxassay_001053_07	euxassay_001053_08	euxassay_001053_09

euxassay_001053_10	euxassay_001053_11	euxassay_001053_12	euxassay_001053_13	euxassay_001053_14

euxassay_001053_15	euxassay_001053_16	euxassay_001053_17	euxassay_001053_18	euxassay_001053_19

euxassay_001053_20	euxassay_001053_21	euxassay_001053_22	euxassay_001053_23	euxassay_001053_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10736_wholemount_strong.wlz

10736_wholemount_moderate.wlz

10736_wholemount_weak.wlz

10736_wholemount_possible.wlz

10736_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10736_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

weak

homogeneous

weak expression: see section 04 05 06 20 21

glossopharyngeal ix ganglion

weak

homogeneous

weak expression: see section 07 08 19

trigeminal v ganglion

weak

homogeneous

weak expression: see section 03 04 05 06 07 08 18 19 20 21 22

vagus x ganglion

weak

homogeneous

weak expression: see section 09 18

dorsal root ganglion

moderate

regional

moderate expression: see section 10 11 17 18 19 weak expression: see section 09 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T185
Entity Detected:	Senp5, SUMO/sentrin specific peptidase 5 ( MGI:2443596)
Sequence:	sense strand is shown >T185 CCACAAGCTTCAGACTTTCCCATGAAGTTCAGTGGGGAGAGCCAAAGTCCAGGTGACAGTGGCAAGACTG TGGTCTTGAACAAACATAGAAAGCGAGTTTGTCATGGCTGCTACCAAGGGCTAGAGCACCACAGGAACAG GAGACCCCTGATTCCAAAGCAGTTCCAGCTTAACCAACACCGAAGAGTCAGAGCGTCTCTGATGATGTAT GAGAAACTATCCATGATTAGATTTCGGTATAGGATTTTCAGATCCCAGCACTTCAGAACGAAAAGCAGAG TTTGCAAGCTAAGAAAGGCCCAGCGAAGTTGGGTACAGAAGGTCACTGGGGACCATCAAGAGAACCTTAG GGATAATAACACTGAGGGTGACAATTGCAATCCAGTCCCTTCCCTAGAACCTAAAGATCCTTGTCGGTGT CAGCCATACTTTCCAGACATGGACAGCAGTGCTGTGGGGAAGGGAAAAAACTGTCACGTGCCTGATGGTC ACACTA
Notes:	The probe template was PCR amplified from IMAGE:2647150 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2647150 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10738 same embryo
	EMAGE:10735 same embryo
	EMAGE:10734 same embryo
	EMAGE:10737 same embryo
	EMAGE:10733 same embryo
	EurExpress:euxassay_001053 same experiment
	MGI:4827953 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source