EMAGE mouse gene expression spatial database

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EMAGE:10751

Ednrb endothelin receptor type B ( MGI:102720)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000978. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000978_01	euxassay_000978_02	euxassay_000978_03	euxassay_000978_04

euxassay_000978_05	euxassay_000978_06	euxassay_000978_07	euxassay_000978_08	euxassay_000978_09

euxassay_000978_10	euxassay_000978_11	euxassay_000978_12	euxassay_000978_13	euxassay_000978_14

euxassay_000978_15	euxassay_000978_16	euxassay_000978_17	euxassay_000978_18	euxassay_000978_19

euxassay_000978_20	euxassay_000978_21	euxassay_000978_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10751_wholemount_strong.wlz

10751_wholemount_moderate.wlz

10751_wholemount_weak.wlz

10751_wholemount_possible.wlz

10751_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10751_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

foregut-midgut junction

moderate

regional

moderate expression: see section 12 13 14 15 16

diencephalon lateral wall ventricular layer

strong

homogeneous

strong expression: see section 11 12 13

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 10 11 12 15 16 17

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

4th ventricle

strong

homogeneous

strong expression: see section 22

medulla oblongata alar plate ventricular layer

strong

homogeneous

strong expression: see section 11 12 13

medulla oblongata basal plate ventricular layer

strong

homogeneous

strong expression: see section 10 11 12 13 14 15 16

metencephalon rest of alar plate ventricular layer

strong

homogeneous

strong expression: see section 04 05 06 07 08 09 15 16 17 18 19 20 21 22

pons ventricular layer

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

midbrain ventricular layer

strong

homogeneous

strong expression: see section 09 10 11 12 13 14 15 16 17

facial vii ganglion

strong

homogeneous

strong expression: see section 05 06 07 20 21 22

inferior glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 09 20

superior glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 09 18

trigeminal v ganglion

strong

homogeneous

strong expression: see section 04 05 06 07 09 10 17 18 20 21 22

vestibulocochlear viii ganglion cochlear component

strong

homogeneous

strong expression: see section 07 20

vestibulocochlear viii ganglion vestibular component

strong

homogeneous

strong expression: see section 05 06 07 09 18 20 21

ventral grey horn

strong

regional

strong expression: see section 13 14 15 moderate expression: see section 16 17

dorsal root ganglion

strong

regional

strong expression: see section 15 16 17 18 19 moderate expression: see section 09 10 11 12 not examined expression: see section 14

esophagus

moderate

regional

moderate expression: see section 13 14

tongue intrinsic muscle

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

stomach

moderate

regional

moderate expression: see section 02 04 05 06 07 08 09 10 11

midgut

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 20

bladder

moderate

regional

moderate expression: see section 13 14

urethra of male

strong

homogeneous

strong expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1064
Entity Detected:	Ednrb, endothelin receptor type B ( MGI:102720)
Sequence:	sense strand is shown >T1064 GTTGGCCTACTGGGACTGAATGCAGACCAGCGGGTGGCGTGCGCCCAAGTTCCCCACTGGCGCGCAAACT TAACTTACTGTTAAGGCGCGGGTAGAGGCAACCGGGCTAGTGTGTTTTCAGAGGCTTGGCTGGGGTAGCT GACTTAAGTGTCCTGTCTTCACTCCCCAGTTGGTCTCCAGACTGAAAACAGCAGAGCGGCTACCAGACTC TCACAGGAGCAAGCTGTAACATGCAATCGCCCGCAAGCCGGTGCGGACGCGCCTTGGTGGCGCTGCTGCT GGCCTGTGGCTTCTTGGGGGTATGGGGAGAGAAAAGAGGATTCCCACCTGCCCAAGCCACGCTGTCACTT CTCGGGACTAAAGAGGTAATGACGCCACCCACTAAGACCTCCTGGACCAGAGGTTCCAACTCCAGTCTGA TGCGTTCCTCCGCACCTGCGGAGGTGACCAAAGGAGGGAGGGGGGCTGGAGTCCCGCCAAGATCCTTCCC TCCTCCGTGCCAACGAAATATTGAGATCAGCAAGACTTTTAAATACATCAACACGATTGTGTCGTGCC
Notes:	The probe template was PCR amplified from IMAGE:2099487 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2099487 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10752 same embryo
	EMAGE:10754 same embryo
	EMAGE:10753 same embryo
	EurExpress:euxassay_000978 same experiment
	MGI:4824475 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source