EMAGE mouse gene expression spatial database

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EMAGE:10766

Tcp11l2 t-complex 11 (mouse) like 2 ( MGI:2444679)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001244. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001244_01	euxassay_001244_02	euxassay_001244_03	euxassay_001244_04

euxassay_001244_05	euxassay_001244_06	euxassay_001244_07	euxassay_001244_08	euxassay_001244_09

euxassay_001244_10	euxassay_001244_11	euxassay_001244_12	euxassay_001244_13	euxassay_001244_14

euxassay_001244_15	euxassay_001244_16	euxassay_001244_17	euxassay_001244_18	euxassay_001244_19

euxassay_001244_20	euxassay_001244_21	euxassay_001244_22	euxassay_001244_23	euxassay_001244_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10766_wholemount_strong.wlz

10766_wholemount_moderate.wlz

10766_wholemount_weak.wlz

10766_wholemount_possible.wlz

10766_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10766_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

arm

moderate

regional

moderate expression: see section 01 02

humerus

strong

regional

strong expression: see section 04 05 21 22 23

hand mesenchyme

moderate

regional

moderate expression: see section 01 02 03 23 24

hindlimb digit 1 mesenchyme

moderate

regional

moderate expression: see section 03 04 23

hindlimb digit 2 mesenchyme

moderate

regional

moderate expression: see section 03 04 23 24

hindlimb digit 3 mesenchyme

moderate

regional

moderate expression: see section 03 04 05 23 24

hindlimb digit 4 mesenchyme

moderate

regional

moderate expression: see section 03 04 05 22 23 24

hindlimb digit 5 mesenchyme

moderate

regional

moderate expression: see section 03 04 05 22 23

foot mesenchyme

moderate

regional

moderate expression: see section 01 02

leg

moderate

regional

moderate expression: see section 01 02 24

femur

strong

regional

strong expression: see section 08 18

cerebral cortex marginal layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22 23

ventral grey horn

moderate

regional

moderate expression: see section 11 13 14 15

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 15 16 17 18

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

genital tubercle of male

moderate

regional

moderate expression: see section 10 11 12

axial skeleton

moderate

regional

moderate expression: see section 12 13 14 15

clavicle

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5382
Entity Detected:	Tcp11l2, t-complex 11 (mouse) like 2 ( MGI:2444679)
Sequence:	sense strand is shown >T5382 GGGATACGCAGAGCCCCGCGACCCCCGCCACCTAGCTTTCCTCGAGTCTCTACGCGGGATCTGGGGGTTC TCTCTCTGTGTGTGTGTGTCTATCTCTCTGTCTCCGTCTCTCTGTCTCTCTGTCTCTCTGTCTCTCTTTC TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTCTCTCTCCCCCCACCCCCATTCCATCCCCAAGGTG ATAAAGGATGCCTTTCAACGGTGAGAAGCAATGTGTGAGCGAGGACCAGCAGAGTGACTCAGAGTCTTCT CGGTTCGCCGAAGGCGTGGCTTCACTCAGTGACTATGAGTGTTCCAGGCAGAGCTTCACCAGCGATTCCT CCAGCAAATCCAGCTCTCCTGCCTCAACAAGCCCTCCCCGGGGCTTAATGTTTGATGATGTGATGGCTGC AGCAAAGAACTTATCAGACATGACTCTCGCTCATGAAATAGCTGTGAATGAGAACTTCCAGCTGAAGCAA AATGCTCTCCCCGAGAACAGCCTTGCTGGTCAAGTGAAGCGTGTTGTCCACCAGGCCTTCTGGGATGTCT TGGAAGCAGATCTCAGTGCTGAGCCTCCTCAGTATGAATATGCCATCAAACTGTTTGAAGAAATCAGAGA GATTCTTCTCTCTTTCCTCACGCCTGGCGGGAACAGACTTCACAGCCAGATCTGTGAGGTTCTGGACATC GACCTCATTAGACAGCAGGCCGAGCACAGTGCTGTCGACATCCAAGGTCTGGCCAACTATGTCATCACCA CCATGGGGAAGATATGTGCTCCTGTGAGAGATGAAGACATCCGAGAGCTGAAAGCTACCACCAACATCGT GGAGATGCTGAGACAAATCTTCCGTGTCTTGGACCTCATGAGAATGGACATGATGAATTTTGTCATTAGG AATATTAGACCTCACATCCAGCATCATTTGGTGGAATATGAGAGAAACAAATTCCAGGAAGTTGTAGAAG AAACTCCAAATGCCCTCAGTCAAACCACAGAGTGGCTGAAAGAGTCCATAGATAAAGAATTGCTTTCTGA GACTGATGTAGCTCCTGCAGCTGAACATAGCTCCACGCCTAGCCTGAGCCCTCTGTTGGTGCTTAATAAC TGTTACTTGAAACTCCTTCAGTGGGATTATCAGAAAAAAGTCTTACCAGAGACACTCATGACAGATGGAC CACGGCTTCAAGAACTGTCAGAAAAGCTCAATCAATTGAAAATGACTGCCTGCGTGGCCTTAATCACCAA CAACATGGTGGGTGCTGTTACAGAAGGCCTGCCTGAACTTGCTAACAGGTTAAAAAGGATTTCAGCTGTT CTGCTTGAAGGCATGAATAAAGAGACCTTTAACTTGAAGGAAGCCCTGCATTCTATTGGTGTTCAGACTT GTGCTGAAGTTAACAAGGCCCTGGAAGAGAGAGGCTCGCCCACTTTAAATGCTGAGGTTCAAGCTAACCT TGTCGGTCAGCTCTCCAGCCTTGAAGAGAAGGACAATCCTGTCTGCACCTTGATGGATAAACGGATCCAG TTGTACATGAAAGGCCTGCTCTGTCTTCCAAGCACTCAGAAGAGCATGCCTCCTGTGCCAGGTGGCCTTG ATGTCATCCAGCGGGAGCTGGAAGTGCTTGGCTGCCAGTATGCAAACATTGTGAATCTCAACAAACAGGT GTATGGACCATTTTATGCAAATATATTCCGAAAGCTGCTCTTCCGGGATGAAGCCGTGGGGAAGATAGAT GCTTCACTTCCCACTAACTAAAGAGACGAGATGGAGATCAGCACTTGGTACATGGTCCGTTTCCACCAGT GGCATTGGAGATGTGGCCCATTCTCCGCCCTGTCCCTAGACTGCATCCGTGTAACCTGGTAATGGATGCT AGCACCACAGAGGGCCTCACACCCCTCTTGTGTTTAAAGCAGATAGTTTTAATGAAGAAAAGTAATTTGT AATGAATTATATTACCTATATAACATTGGTGTTTTCTTAAACATTTATATTGAGATTTTGTTGTTGTTGT TTTGGACAGTGTGTCTGTGTAACAGCCCTGGCTGTCCTGGAGTATATTGCACATTTCTATGTGACTTGTT CATTCAACCTGAAAGGAAATGATTTTTGTTTATATACTTGATTATACTGCAGCTGCCTGCAGGAATACAA TGTGCAAACATTTAAAAATACATTGAAAATGAAGGGCATTCTGCTTCATTTTTTGTTAAGTGGCAAAGTA AATTTGTGTCTTGGATATGGATTTAGCCAAGTACATTTATTAGGGTTTTTTAGAATCATAGCTCTTGGCA TGTTTATTTAGTTTTTACTTAAATGTTTACTTCAACTTTTGTGGCTTTCCTAACATGGTTGTTTTATAAT AAAAACTTTATTAAACAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5364434 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5364434 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10769 same embryo
	EMAGE:10767 same embryo
	EMAGE:10765 same embryo
	EMAGE:10768 same embryo
	EurExpress:euxassay_001244 same experiment
	MGI:4828647 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source