EMAGE mouse gene expression spatial database

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EMAGE:10769

Wdr31 WD repeat domain 31 ( MGI:1918604)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001283. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001283_01	euxassay_001283_02	euxassay_001283_03	euxassay_001283_04

euxassay_001283_05	euxassay_001283_06	euxassay_001283_07	euxassay_001283_08	euxassay_001283_09

euxassay_001283_10	euxassay_001283_11	euxassay_001283_12	euxassay_001283_13	euxassay_001283_14

euxassay_001283_15	euxassay_001283_16	euxassay_001283_17	euxassay_001283_18	euxassay_001283_19

euxassay_001283_20	euxassay_001283_21	euxassay_001283_22	euxassay_001283_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10769_wholemount_strong.wlz

10769_wholemount_moderate.wlz

10769_wholemount_weak.wlz

10769_wholemount_possible.wlz

10769_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10769_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

homogeneous

weak expression: see section 11 12 13 14 15

submandibular gland primordium

weak

regional

weak expression: see section 08 09 10 17

foregut-midgut junction

weak

regional

weak expression: see section 11 12 13 14 15 16

pancreas

weak

regional

weak expression: see section 09 10 11 16

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 06 07 08 09 10 12 13 15 16 17 18

telencephalon ventricular layer

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21

4th ventricle

moderate

regional

moderate expression: see section 17 18 weak expression: see section 05 06 07 08 09 10 11 13 14 15 16 19 20

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 08 09 10 11 12 14 15 16 17

anal canal

weak

regional

weak expression: see section 13

stomach fundus mucosa

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10

hindgut

weak

regional

weak expression: see section 12 13 14

midgut

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16

not examined

regional

not examined expression: see section 18

renal cortex

weak

regional

weak expression: see section 07 08 09 10 16 17 18

testis

weak

regional

weak expression: see section 05 06 07 08 17 18 19

urethra of male

weak

regional

weak expression: see section 12 13 14

left lung

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11

right lung

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5378
Entity Detected:	Wdr31, WD repeat domain 31 ( MGI:1918604)
Sequence:	sense strand is shown >T5378 CCACGCGTCCGGTGGAAGCTAAGGATGCCTGCTACCCGTGCTTTCGGGCCTCGGTGCTAGCCCACCGAGA TTAGGAAGTGGCTGTCTGACTTGTTTCCGAAGGCTGAATGAACTACAGATCATCAAATACAGCAGCTCAC CAAGAAGATCTGGTTGCTTTGGGGGGAACACCCCCTCAGCGCCTGTGAGGGATGTTGTTACTTGACGGCC AACTGAAACCAGCTCTTCCTCAGAAGGTCTTCTGTAGACTCTGGGCTGTGATGGGAAAACTGCAGAGCAA ACTCAGACACAGCACTTACTTATACAGCAGGCCTGATGGAATTATAGAAGAGAGAGTCCAGACTAAAGCT CCCCAAGAATACATCCCTGTTCACGTGGATACCGTCTCTGTGATTGCCACTCTGAACTCAGACCTTTGTA TCTCTGGAGGAAAAGACAAGACCGCCGTGGCCTACAATTGGAAAACTGGGCGTATGGTGAAAAGGTTCAC AGGGCACGAGCGGGAAATCACGAAGATAGCCTGTATTCCCAAAGCAAACCAGTTCTTCAGCGCCTCTCGA GACAAGACAGTCCTGATGTGGGATCTGCAGGGCTCCTCGCACCCTAGGCAGCAGCTGTCTGGCCATGCCA TGGTGGTCACTGGCTTGGCTGTGAGCCCAGACTCGTCCCAGCTGTGCACTGGCTCTCGGGACAATAGCCT GCTTCTGTGGGATGTGGGGACCGGACAGTGTGTGGAGAGAGCGTCTGTCTCCAGGAACCTGGTCACACAC CTGTGCTGGGTCCCGAGTGAGCCATACATAGTCCAGACCTCTGAGGATAAGACCATCAGATTATGGGACA GTCGGGGGCTTCAGATGGCTCACATGTTTCCCACAAAACAGCATATCCAGACCCATTGTGAAGTCAGCGT GGATGGACACACGTGTATTTCCTGCAGCAGTGGCTTTGGGGGTGAAGGCTGTGAAGCCACGTTGTGGGAC CTAAGACAGACTAGAGACAGAATGTGTGAGTATAAGGGGCATTTCCAGACAGTTGCATCCTGTGTCTTTC TACCGAAGTCCACGGCCTTGATGCCTATGATTGCTACCTCTGCACATGACTCCAAGGTGAAGATTTGGAA CCAGGATACTGGAGCTTGCCTCTCCACCTTGTCTCTGGATGGATCAGGACCCCTGACCTCACTGGCTGTT GGTGACATCTCCTCCTTGTTATGCGCAAGTTTCTACAGAGGAGTTCACTTACTCAGAGTGGACCACAGCC AAGGACTGGAACTGCAGGAAGTGGCAGCCTTCTAAAATCAAGAGATATTGATTGGATAATATCAAACCAT GGTCCCTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5364307 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5364307 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10766 same embryo
	EMAGE:10767 same embryo
	EMAGE:10765 same embryo
	EMAGE:10768 same embryo
	EurExpress:euxassay_001283 same experiment
	MGI:4829196 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source