EMAGE mouse gene expression spatial database

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EMAGE:10789

Serpinb1b serine (or cysteine) peptidase inhibitor, clade B, member 1b ( MGI:2445361)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003215. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003215_01	euxassay_003215_02	euxassay_003215_03	euxassay_003215_04

euxassay_003215_05	euxassay_003215_06	euxassay_003215_07	euxassay_003215_08	euxassay_003215_09

euxassay_003215_10	euxassay_003215_11	euxassay_003215_12	euxassay_003215_13	euxassay_003215_14

euxassay_003215_15	euxassay_003215_16	euxassay_003215_17	euxassay_003215_18	euxassay_003215_19

euxassay_003215_20	euxassay_003215_21	euxassay_003215_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10789_wholemount_strong.wlz

10789_wholemount_moderate.wlz

10789_wholemount_weak.wlz

10789_wholemount_possible.wlz

10789_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10789_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 13 moderate expression: see section 12 14 15

foregut-midgut junction

weak

regional

weak expression: see section 06

pancreas

strong

regional

strong expression: see section 10 11 12 13 14

nervous system

weak

regional

weak expression: see section 01

diencephalon

weak

regional

weak expression: see section 05

physiological umbilical hernia dermis

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

3rd ventricle

strong

regional

strong expression: see section 13 moderate expression: see section 12

epithalamus

weak

regional

weak expression: see section 11

diencephalon meninges

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19

thalamus ventricular layer

weak

regional

weak expression: see section 11 12 13

lateral ventricle choroid plexus

strong

regional

strong expression: see section 06 07 08 09 10 11 weak expression: see section 05

telencephalon meninges

moderate

regional

moderate expression: see section 02 03 04 05 06 weak expression: see section 01 13 19 20 21 22

4th ventricle

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 moderate expression: see section 12 weak expression: see section 01

midbrain mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 15 16 weak expression: see section 05 10 11 12 13 14

midbrain ventricular layer

moderate

regional

moderate expression: see section 13 weak expression: see section 10 11 12

dorsal grey horn

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 weak expression: see section 18

naris

strong

regional

strong expression: see section 13 16 moderate expression: see section 14 15 17 weak expression: see section 12

tongue

moderate

regional

moderate expression: see section 13 weak expression: see section 11 12 14 15 16

hindgut

moderate

regional

moderate expression: see section 14 15 16 17 18

rectum

strong

regional

strong expression: see section 15

midgut

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 weak expression: see section 12

midgut loop

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 weak expression: see section 06

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 16 weak expression: see section 15 17

upper jaw incisor

moderate

regional

moderate expression: see section 12 13 16 weak expression: see section 15 17

liver

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5417
Entity Detected:	Serpinb1b, serine (or cysteine) peptidase inhibitor, clade B, member 1b ( MGI:2445361)
Sequence:	sense strand is shown >T5417 CCACGCGTCCGGCTTCTTCCTAGGTCTCTAGCAAGTGGAGCCAGAGCTGCTACAGAGGAGCCTTCACCAT GGAGCAGCTGAGTTCAGCGAACACCCTCTTCACCTTGGAGCTGTTCCACACACTGAAGGAAAGCAGCCCC ACAGGAAACATCTTCTTCTCTCCATTTAGTATTTCTTCTTCCTTGGCCATGGTCTTTCTGGGAGCCAAAG GCAGCACTGCAGCCCAGCTCTCTAAGACTCTTCATTTTGACTCTGTTGAGGACATCCATTCATGCTTTCA AAGCCTGACTGCTGAAGTGAGCAAGCTGGGAGCATCTCACACTCTGAAACTTGCTAACAGACTGTATGGA GAGAAAACCTACAATTTCCTTCCTGAGTTCTTGGCTTCCACCCAGAAAATGTACAGTGCTGACTTGGCCG CTGTGGATTTTCAGCATGCCTCTGAGGATGCAAGGAAGGAGATAAACCAATGGGTCAAAGGTCAAACAGA AGGTAAAATCCCAGAACTGCTGGCTAAGGGTGTGGTAGACAGCATGACCAAACTTGTGCTAGTGAATGCC ATTTACTTCAAAGGAATATGGGAGGAGCAATTCATGACAAGAGAAACAATCAATGCTCCATTCAGACTGA ATAAGAAAGACACAAAAACAGTGAAGATGATGTATCAAAAGAAAAAATTTCCATTCGGTTACATTTCGGA CCTGAAGTGCAAGGTGCTGGAGATGCCTTACCAGGGTGGAGAACTTAGCATGGTCATTCTGCTGCCTGAA GACATTGAGGATGAGTCCACGGGTCTTAAGAAGATTGAGGAGCAATTAACTTTGGGAAAACTTCATGAAT GGACCAAACATGAGAACTTGAGAAACATTGATGTCCATGTCAAATTGCCCAGGTTCAAGATGGAAGAGAG CTACATACTCAACTCTAACCTGTGCTGCCTGGGAGTGCAGGATCTCTTTAGTAGTGGCAAGGCTGATCTC TCTGGCATGTCAGGATCCAGAGATCTTTTCGTATCAAAAATTGTCCACAAGTCATTTGTGGATGTTAATG AGCAGGGAACAGAGGCAGCTGCTGCCACAGGAGGCATTATTCAGGTGCTCTGCGAGAAGATGCCTACTCC ACAGGAAGTATTCACTGTGGATCACCCATTCCTTTTCTTTATTCGGCACAACCCCACAGCTAATATGATC TTCTTTGGCAGGGTTTGTTCCCCATAGAAGAAGGAGACTTTAGAGATAGAAGGCAGAGCTTAGCATTTCA TTCCCTGAGATTAATGATGATTATTCCATTAGTCCTTGTCAATAAAACCTCTATACAAAACACAATCATT CTTTCTTTTGTATGTGTGTATGTTTTGTATGTTTCTTTTGTATGTTGGCTTTTTATATCCATTGGCATGG GTATGTATATATTGTTTTTCCCCTGAAAAATCTAATTTCCACTGTCAACAGAATCACGTCTAAAAGCTAA AGAATTAAATAGATGTATAGATTCATGACTGTGAGGTAACCTGTGCAATTGTAATAGGCTCCTGATAGGC ATGAAAATGTATGGCTTTTCCCATTCAATTGTTTTCCAATTTAGAATTAGAGTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5367365 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5367365 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10790 same embryo
	EurExpress:euxassay_003215 same experiment
	MGI:4827990 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source