EMAGE mouse gene expression spatial database

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EMAGE:10851

Fam108c family with sequence similarity 108, member C ( MGI:1917428)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003396. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003396_01	euxassay_003396_02	euxassay_003396_03	euxassay_003396_04

euxassay_003396_05	euxassay_003396_06	euxassay_003396_07	euxassay_003396_08	euxassay_003396_09

euxassay_003396_10	euxassay_003396_11	euxassay_003396_12	euxassay_003396_13	euxassay_003396_14

euxassay_003396_15	euxassay_003396_16	euxassay_003396_17	euxassay_003396_18	euxassay_003396_19

euxassay_003396_20	euxassay_003396_21	euxassay_003396_22	euxassay_003396_23	euxassay_003396_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10851_wholemount_strong.wlz

10851_wholemount_moderate.wlz

10851_wholemount_weak.wlz

10851_wholemount_possible.wlz

10851_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10851_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 11 17 18 19 20 21

pancreas

moderate

regional

moderate expression: see section 11 12 13 14 18 19 20

epidermis

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

strong

regional

strong expression: see section 06 07 08 21 22 23 24

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 21 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 18 19 20 21 22 23

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 09 18 19 20

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 17 18 19 20

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 04 24

esophagus

moderate

regional

moderate expression: see section 13 14

stomach

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11

hindgut

moderate

regional

moderate expression: see section 15 16 17 18 19 20 21

rectum

moderate

regional

moderate expression: see section 15 16

midgut

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22

midgut loop

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 16 17 18

upper jaw incisor

moderate

regional

moderate expression: see section 12 13 14 17 18

bladder

weak

regional

weak expression: see section 14 15 16

kidney calyx

moderate

regional

moderate expression: see section 10 11 12 20 21 22 23

testis

weak

regional

weak expression: see section 08 09 22 23 24

urethra of male

weak

regional

weak expression: see section 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5939
Entity Detected:	Fam108c, family with sequence similarity 108, member C ( MGI:1917428)
Sequence:	sense strand is shown >T5939 GCGCTCCCTGCGCTTCCCTCCCGCCCGCGGCGGGCGCCAGGCCGCCCCGATGCCCGAGCCCGGCCCCAGG ATGAACGGCTTCTCGCTCGGCGAGCTGTGCTGGCTCTTCTGCTGCCCTCCTTGTCCGAGCCGCATCGCCG CCAAGCTGGCCTTCCTGCCGCCCGAACCCACCTACACGGTGCTGGCGCCCGAGCAGCGCGCGCCCGCTCC CGCGGCGACCCCCGCGCCCGCCCCGGCGGCGCAGCCCGCTCCGGCCGAGGAGGGTGCGGGCCCCGGCGCG TGCAGCCTGCACCTGAGCGAGCGCGCCGACTGGCAGTACTCGCAGCGCGAACTGGACGCCGTCGAGGTCT TCTTCTCGCGCACGGCCCGCGACAACCGGCTGGGCTGCATGTTCGTGCGCTGCGCGCCCTCCAGCCGCTA CACGCTGCTCTTCTCGCACGGCAACGCCGTGGACTTGGGCCAGATGTGTAGCTTCTACATCGGCCTGGGC TCGCGCATCAACTGCAACATCTTCTCTTACGACTACTCGGGCTATGGTGTGAGCTCCGGCAAGCCCTCCG AAAAGAATCTCTACGCTGACATCGACGCCGCGTGGCAGGCACTGCGCACCCGGTATGGCGTGAGTCCTGA GAACATCATCCTCTATGGACAGAGCATTGGCACTGTCCCCACTGTGGACCTGGCCTCGCGGTACGAGTGT GCAGCCGTCATCCTCCATTCCCCACTGATGTCTGGTTTGCGTGTTGCTTTTCCGGATACCAGGAAAACAT ACTGCTTTGATGCTTTCCCCAGCATTGACAAGATATCTAAAGTCACCTCTCCTGTGTTGGTCATTCACGG TACCGAGGATGAGGTCATCGATTTCTCCCATGGCTTGGCGATGTATGAGCGCTGTCCCCGTGCTGTGGAG CCCCTGTGGGTTGAGGGGGCTGGGCACAATGATATAGAGCTCTATGCACAGTACCTAGAACGACTAAAAC AGTTCATATCTCACGAGCTTCCCAACTCCTGAAGACAGCGGCCAACCTTACCTCATTACTGTGAAGCGAG GATCCTTGTTTGCACACGCTTTGACTGGATTTAACAGTGATACCCAATAACCACAGAGAAGGGCCCAGCC TTTGTGTGTTCTTCTTAAAGAGCTAACACAGTCTGTCTTTTGTACGTAATCTCCACCTTCCATTAGGCTG AGCAGCTGGATTCTCAGCTGCAAGACCTTGCAGGAGTGGGAACGAGAGCTGACCATGAGGACTGGTTCCC AGGGCTGTAGACTAAAGTGTGATCAAGTCCGTCTCCCTTGCTGTCTGTCACCTCTGATTCCCCTACCGGT GCAGGATCACCTCTCATCCATGTGCCGGTGTTTGACCTGCGGCGCTTTCAGCCCTGGTGCCATGGGTGAG ATTCATATGTGAAGCTATGGTAGTGTAACTGGAAACTGTCGTGAGAAATCCTCCATTAACTTGTATTAAC ACGCCAGCCTTTCCCACACACATGCATTGAAGAATGATTTACTGGAACTATAACAGCTTCATCAACTGTT ACCATTTAAATACAACTGGAATATTCTTAAACAACAGGAAACTGGGGTTTTTTTAAGTGTAAATTTATTA CTAGCCAAGAGTTTTAATATTTTGATTGTCTGGTCTAATAGAGCCTGGCCGACTTTCCTTCTGTAAAGTC CTCCTTGTCGGCTTTCTTAAAAGTCCTGTACATATTTGCAATTACATTGTGCACAGATTCTTAATGGATA GATTTCTTTTGTCAGGCTACAACAACAGACTGCAGATTCCTTGTTTGTATTGTAAATGATTGAATACATT TTGTTAATATGTTTTTATTCCTATGTTTTGCTATTAAAATTTTTATAACATTTCCAAGACAAAAATTCCA AGTTTCTGCTTTGAAGAATTTAGGTAATTAAATTACCCTAAGCTGATCTCTTAGTTTAGGAGTTATTTGG GTTTTGACACTGGAGTTGTGCCAGGTGAACATCAGAAACAAGATGCTTAAATCATTACACTAACTTGTAT TGGTTGCGGGATTGGGTTTGGGGTCGTTTGGTCTTATTTTAGGGTTTTTTTTTTTTTGTTTTTTTTCTTC TTCAAATTTAAAAGCCTTAAATGTACTGTAAGCCTCAGATCGTTGTACAACTGGATTGCGTTGGTTGCCA GTTTGTGTGCTGTTGCCTGGATGCGGCACGGTGGTTGGTAATGGAATAAAGGACGCATGGATCAAAAAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3483379 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3483379 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10846 same embryo
	EMAGE:10848 same embryo
	EMAGE:10850 same embryo
	EMAGE:10849 same embryo
	EMAGE:10847 same embryo
	EurExpress:euxassay_003396 same experiment
	MGI:4824688 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source