EMAGE mouse gene expression spatial database

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EMAGE:10903

Tacc3 transforming, acidic coiled-coil containing protein 3 ( MGI:1341163)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003324. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003324_01	euxassay_003324_02	euxassay_003324_03	euxassay_003324_04

euxassay_003324_05	euxassay_003324_06	euxassay_003324_07	euxassay_003324_08	euxassay_003324_09

euxassay_003324_10	euxassay_003324_11	euxassay_003324_12	euxassay_003324_13	euxassay_003324_14

euxassay_003324_15	euxassay_003324_16	euxassay_003324_17	euxassay_003324_18	euxassay_003324_19

euxassay_003324_20	euxassay_003324_21	euxassay_003324_22	euxassay_003324_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10903_wholemount_strong.wlz

10903_wholemount_moderate.wlz

10903_wholemount_weak.wlz

10903_wholemount_possible.wlz

10903_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10903_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 17 moderate expression: see section 10 16

hypothalamus ventricular layer

strong

regional

strong expression: see section 10 11 12 13 14

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 11 12 13 14

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 12 13 17 18

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata basal plate ventricular layer

strong

single cell

strong expression: see section 09 10 11 12 13

metencephalon rest of alar plate marginal layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 13 14 15 16 17 18 19

metencephalon rest of alar plate ventricular layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 13 14 15 16 17 18 19

midbrain ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14

ventral grey horn

moderate

single cell

moderate expression: see section 09 10 12 13 14 15

optic stalk

moderate

regional

moderate expression: see section 10 11 12 14 15 16

optic chiasma

moderate

regional

moderate expression: see section 11 12

nucleus pulposus

moderate

regional

moderate expression: see section 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6245
Entity Detected:	Tacc3, transforming, acidic coiled-coil containing protein 3 ( MGI:1341163)
Sequence:	sense strand is shown >T6245 GTTGGGGTTCCTTCGGCTGGGTTTCTCCTGCTCCGTTTTCTTAGCGCGAAGCGGGCACGGCGCTCCTCCC TTGCGGTGGGCGTCCGCGCGGGCCCAGGGCTTGGCAACCGTAGTCAGAATGAGTCTGCATGTCTTAAATG ACGAAAACGTGCCCAATGAAAAGAGTTCACAGTGCCGTGATTTCCAGTTTTTGCCACCAGAACTTACCGG CAGATCATCTGTTCTTTGTCTGTCACAGAAAGAAAATGTACCACCCCAGAGCCAGGCTAAGGCCACAAAT GTGACGTTCCAGACACCACCTCGGGATCCACAGACACACAGGATCTTAAGTCCTAACATGACCAATAAGC GTGAGGCTCCTTTTGGATTACAAAATGACCACTGTGTCTTCTTACAGAAAGAAAACCAGCGGCCTTTAGC CCCAGTGGATGATGCACCTGTGGTTCAAATGGCAGCTGAGATCCTAAGAGCAGAAGGCGAGTTACAGGAG GGAATCTTGACTTCCTCAAGCCTTTCAGCTTCCACAAGTCTTCTGGACAGTGAGCTGGTGACCCCTCCTA TCGAGCCAGTGCTGGAGCCTTCCCATCAGGGACTAGAACCTGTCTTGGAAAGTGAGCTGGTGACCCCTCC TGTTGAGCCAGTGCTGGAGCCTTCCCATCAGGAACTAGAACCTGTCTTGGAAAGTGAGCTGGTGACCCCT CCTATTGAGCCAGTGCTGGAGCCTTCCCATCAGGGACTAGAACCTGTCTTGGACAGTGAGCTGGTGACCC CTCCTATTGAGCCAGTGCTGGAGCCTTCCCATCAGGGACTAGAACCTGTCTTAGAAAGTGAGCTGGTGAC CCCTCCTATTGAGCCAGTGCTGGAGCCTTCCCATCAGGGACTAGAACCTGTCTTGGACAGTGAGCTGGTG ACCCCTCCTATTGAGCCAGTGCTGGAGCCTTCCCATCAGGGACTAGAACCTGTCTTGGACAGTGAGCTGG TGACCCCTCCTATCGAGCCTTTGCTGGAGCCTTCCCATCAGGGACTAGAACCTGTCGTGGATCTAAAGGA AGAGAGCTTCAGAGACCCATCAGAAGTCCTAGGTACTGGTGCTGAAGTGGATTATCTGGAGCAGTTTGGA ACTTCCTCGTCCCATCTCTCCAACTCTCAGTTTAAGGAGTCGGCCTGGAGGAAGCAGTCTTTGTACGTGA AATTTGACCCTCTTCTGAAAGACAGCCCTTTGCGGCCAATGCCGGTGGCCCCAATAACAAATAGTACACA GGATACAGAGGAGGAGTCTGGTTCTGGAAAGCCGACAGAAGCTGAGCTTGTCAACTTAGATTTCTTGGGA GATTTGGATGTTCCGGTATCTGCCCCACCCCTGTGTGTCCTTGAGCCTCGAGGTCTCCTTCCTGCTGAGC CTATTGTGGATGTGCTGAAGTACAGCCAGAAAGACCTGGATGCAGTGGTGAATGTGATGCAGCAGGAGAA CTTGGAGCTGAAAAGCAAGTATGAGGACCTCAACACAAAATATTTGGAGATGGGGAAGTCCGTTGATGAG TTTGAGAAGATCGCGTACAAATCATTGGAGGAGGCTGAGAAACAGAGGGAACTAAAGGAAATAGCTGAAG ACAAAATCCAAAAGGTTTTAAAAGAGAGAGACCAACTTAATGCAGACCTGAACTCCATGGAAAAGTCCTT TTCTGACCTTTTCAAACGGTTTGAGAAGCGGAAGGAAGTGATTGAAGGCTACCAGAAGAATGAAGAGTCA CTGAAGAAGTATGTTGGGGAATGCATAGTGAAAATTGAAAAAGAAGGCCAGAGGTACCAGGCGCTGAAGA TCCATGCAGAAGAAAAGCTCAGGCTGGCAAATGAGGAGATCGCCCAGGTACACAGCAAGGCCCAAGCAGA GGTACTGGCTTTGCAGGCAAGCCTGAGGAAGGCACAAATGCAGAACCACTCGCTGGAGATGACTCTGGAG CAGAAGACAAAAGAAATTGATGAGCTGACCCGGATCTGTGATGACCTCATCTCTAAGATGGAGAAGATCT GAGCACAGCCAGCTATAACCAGCGCTCTTCTGTCTGTCTACCCATTTATTTCCTTATCTGTCTGATTTAT CTCTTACGTGTGGTTTATTTAACCTCTGTTCATTTCTAAATTACTTTATTTTGAAAAGACTAAAGTTTAA TCTCAAACTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3500413 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3500413 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10902 same embryo
	EMAGE:10901 same embryo
	EMAGE:10906 same embryo
	EMAGE:10905 same embryo
	EMAGE:10904 same embryo
	EurExpress:euxassay_003324 same experiment
	MGI:4828581 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source