EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10913

Pax9 paired box gene 9 ( MGI:97493)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003664. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003664_01	euxassay_003664_02	euxassay_003664_03	euxassay_003664_04

euxassay_003664_05	euxassay_003664_06	euxassay_003664_07	euxassay_003664_08	euxassay_003664_09

euxassay_003664_10	euxassay_003664_11	euxassay_003664_12	euxassay_003664_13	euxassay_003664_14

euxassay_003664_15	euxassay_003664_16	euxassay_003664_17	euxassay_003664_18	euxassay_003664_19

euxassay_003664_20	euxassay_003664_21	euxassay_003664_22	euxassay_003664_23	euxassay_003664_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10913_wholemount_strong.wlz

10913_wholemount_moderate.wlz

10913_wholemount_weak.wlz

10913_wholemount_possible.wlz

10913_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10913_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 24

foot mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 22 23 24

thymus primordium

strong

homogeneous

strong expression: see section 11 12 13 14 15

sublingual gland primordium

strong

regional

strong expression: see section 02 03 23

submandibular gland primordium

strong

regional

strong expression: see section 06 07 08 09 10 16 17 18 19 20

thyroid gland

strong

regional

strong expression: see section 10 15 16

nasal septum

strong

regional

strong expression: see section 11 12 13 14 15 16

viscerocranium

strong

regional

Expression in the turbinate bone.

esophagus

strong

regional

strong expression: see section 12 13

mandible

strong

regional

strong expression: see section 03 04 05 06 07 16 19 20 21 22 23

lower jaw incisor

strong

regional

strong expression: see section 11 12 13 14 15 16 17

palatal shelf

strong

regional

strong expression: see section 04 05 06 07 08 09 13 19 20 21 22 23

upper jaw incisor

strong

regional

strong expression: see section 11 12 13 15 16 17

urethra of male

moderate

regional

moderate expression: see section 12 weak expression: see section 13

thyroid cartilage

strong

regional

strong expression: see section 12 13

axial skeleton

strong

regional

strong expression: see section 10 11 12 13 14 15 moderate expression: see section 07 08 09 16

axial skeleton tail region

strong

regional

strong expression: see section 10 11 12 13 14 15 moderate expression: see section 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6780
Entity Detected:	Pax9, paired box gene 9 ( MGI:97493)
Sequence:	sense strand is shown >T6780 CTTCCTTTTACTCTTTCCTTTGGCACTCTTGTCGCCTCCTCCCGGGAAGAAGCCAAGGCACCCTAGGCTT GGAGCAGCGACAGGCCGGCTCAGTGAGAACAAGAAAAAAGTTTCTTTCTGGGAGTGCGGAACTGGGGCCG GGTTGGTGTACTGCTCAGAGCAATGGAGCCAGCCTTCGGGGAGGTGAACCAGCTGGGAGGAGTGTTCGTG AACGGAAGGCCGCTGCCCAACGCCATTCGGCTTCGCATCGTGGAATTAGCCCAACTGGGCATCCGACCTT GTGACATCAGCCGCCAGCTACGGGTCTCGCACGGCTGCGTCAGCAAGATCCTGGCGCGCTACAACGAGAC CGGTTCGATTTTGCCGGGAGCTATTGGGGGGAGCAAGCCCCGGGTCACCACCCCTACTGTGGTGAAACAC ATCCGGACTTACAAGCAGAGGGACCCAGGCATCTTCGCTTGGGAGATCCGGGACCGCCTGCTGGCGGATG GCGTGTGCGACAAGTACAACGTGCCCTCGGTGAGTTCCATCAGCCGTATTCTGCGCAACAAGATCGGCAA CTTGGCCCAGCAGGGTCATTACGACTCTTACAAGCAGCACCAGCCCGCGCCGCAGCCCGCGCTGCCCTAC AACCACATTTACTCATATCCCAGTCCCATCACGGCGGCAGCAGCTAAGGTGCCTACACCACCTGGGGTGC CGGCCATCCCCGGATCGGTGGCCTTGCCGCGCACCTGGCCCTCCTCTCACTCCGTCACGGACATTCTGGG CATCCGCTCCATCACCGACCAAGGAGTGAGCGACAGCTCCCCCTACCACAGCCCCAAGGTGGAGGAGTGG AGCAGCCTGGGCCGCAACAACTTCCCTGCCGCCGCCCCGCACGCAGTGAATGGATTGGAGAAGGGAGCCT TGGAGCAGGAAGCCAAGTACGGCCAGGCACCGAATGGTCTCCCAGCTGTGAGCAGTTTCGTCTCAGCATC CAGCATGGCTCCTTACCCTACACCAGCCCAAGTGTCACCCTACATGACCTACAGTGCTGCTCCTTCTGGT TATGTGGCTGGACATGGGTGGCAACATGCCGGCAGCACCCCACTGTCCCCCCACAACTGTGACATTCCCG CATCGCTGGCTTTCAAGGGAATGCAGGCAGCCAGGGAAGGCAGCCATTCTGTGACCGCTTCTGCACTCTG ATGGGAGCGTCCACTCCGGCAGCCCCACTTCCGGTCTTCCCTCCTAGCACATCCTCCCTCTCGTCACACC CCACCCCTCCAGTGGTCCAGCTTTACTGCCTCAAGAGCTGGCTTCATGGATTCATAATCTTTGTGCTGAT GACACTTAAATATTTTTTTTTTTTTTGCTGTAACTTCCCTTTTCCAGGAAACCTGGCATAACTTTAGGAT TTAAAAACAAAAGCAACTCTAAAGGTGGAATGAGGCATTTGTGTTGCCCGCACACTGTTTTAACACAGAG AAGAAACCTATCCCCCTCAAAGGGCTTATGGAACATTGTAGGTCTGTCTTTGGTAAAACCACACCTGTAT ATTTATTCTAAATCAACCTGAACTTTCGAAACGTGCAATTGTTGAGATTTTGCAAAATCAATAAAGGAAA ACAGATGTAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3707718 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3707718 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10912 same embryo
	EMAGE:10911 same embryo
	EurExpress:euxassay_003664 same experiment
	MGI:4827068 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source