EMAGE mouse gene expression spatial database

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EMAGE:10960

Orc5 origin recognition complex, subunit 5 ( MGI:1347044)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003489. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003489_01	euxassay_003489_02	euxassay_003489_03	euxassay_003489_04

euxassay_003489_05	euxassay_003489_06	euxassay_003489_07	euxassay_003489_08	euxassay_003489_09

euxassay_003489_10	euxassay_003489_11	euxassay_003489_12	euxassay_003489_13	euxassay_003489_14

euxassay_003489_15	euxassay_003489_16	euxassay_003489_17	euxassay_003489_18	euxassay_003489_19

euxassay_003489_20	euxassay_003489_21	euxassay_003489_22	euxassay_003489_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10960_wholemount_strong.wlz

10960_wholemount_moderate.wlz

10960_wholemount_weak.wlz

10960_wholemount_possible.wlz

10960_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10960_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 11 12 13 14 15

submandibular gland primordium

strong

regional

strong expression: see section 08 09 17 19 moderate expression: see section 06 07 10 16 18 20

pancreas

moderate

regional

moderate expression: see section 08 09 10 11 15 16

thyroid gland

moderate

regional

moderate expression: see section 11 12 15

adenohypophysis

moderate

regional

moderate expression: see section 10 11 12 13 14 15

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 08 09

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 12 16 17 18

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 14 15 16 17 18

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 11 12 14 15 16 17

midgut

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 12 15 16 17

lower jaw molar

moderate

regional

moderate expression: see section 05 06 20

upper jaw incisor

moderate

regional

moderate expression: see section 10 11 12 15 16

upper jaw molar

moderate

regional

moderate expression: see section 05 06 20

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

metanephros

moderate

regional

moderate expression: see section 07 08 09 10 11 17 18 19 20 21

genital tubercle of male

weak

regional

weak expression: see section 20

testis

moderate

regional

moderate expression: see section 06 07 22 23 weak expression: see section 21

lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

not examined

regional

not examined expression: see section 09 10 11 12 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6688
Entity Detected:	Orc5, origin recognition complex, subunit 5 ( MGI:1347044)
Sequence:	sense strand is shown >T6688 GGTTGTTCTACGTGAACCACGCCTGAGTGGCGCAGGGCTGACCGGAGGCCAACTGCTCTGCAGTATCAGG TGGTGCCCGCCAAAATGTCTCACTTGGAGAGCATGGTCCTCTGTCGAGAGGCCCAAGTGTCCACTTTGCA GTCCCTGTTTGGAGAGAGACATCATTTCAGCTTTCCGTCCATCTTTATTTATGGACACACTGCCAGTGGG AAGACCTATGTAACACAAACATTGTTGAAAACTTTAGAGCTGCCGCATGCCTTTGTGAATTGTGTTGAAT GTTTTACTTCGAGGCTACTTTTGGAACAAATTTTAAACAAGCTGAGTCATCTTAATTCTTCAGATGCAGG GTGTTCTACAGAAATGACCTGTGAAACATTTAATGACTTTGTTCAGTTATTTAAGCAAGTGACCAGTGCT GAACATCTCAAGGACCAGACTGTGTATATTGTTCTGGATAAAGCTGAGTATCTAAGAGACATGGAAGCAA ATCTTTTGCCTGGGCTCCTTAGGCTGCAAGAATTGACTGACAGAAATGTGACTGTTATCTTTCTCAGTGA GATTATTTGGGAAAAATTTCGTCCAAATACTGGATGCTTTGAGCCATTTGTCTTATATTTCCCTGACTAT AGCATAGGAAACCTTCAAAAGATCCTGTCCCATGATCATCCTCCAGAGTATTCAGCGGATTTTTACGCTG CCTACATTAACATTCTCCTTGGAGTTTTCTACACTGTCTGTCGGGATCTGAAAGAACTCAGACACCTGGC AGTGCTTAATTTCCCCAAATACTGTGAACCTGTAGTTAAAGGAGAAGCAGGTGAACGTGATACTCGCAAA CTGTGGAGAAATATTGAACCTCACCTGAAGAAGGCAATGCAGACTGTGTATCTCAGGGAGATCTCAAGTT CCCAGTGGGAAAAGTTACAGAAAGATAACACAGACCCAGGACAACTGAAAGGTCTCTCGGCGTACACCCA CATGGAACTTCCATATTACTCCAAGTTTATTCTTATTGCTGCATACCTTGCCTCTTATAACCCAGCGAGA ACTGATAAGAGGTTCTTCCTTAAGCATCATGGGAAAATCAAGAAGACTAACTTTCTAAAGAAGCATGAGA AGACAAGCAATCATCTTCTTGGACCAAAGCCCTTTCCACTGGACAGATTATTAGCAATATTGTATAGTAT AGTGGACAGCAGAGTGGCTCCAACAGCAAATATCTTCTCCCAGATTACCTCCCTAGTGACTCTACAGCTG TTAACCATAGTTGGTCATGAAGATCAACTTAATGGGCCAAAATACAAATGCACAGTTTCTCTAGATTTCA TCAGAGCTATTGCACGGATGGTGAACTTTGACATAATAAAATACTTGTATGATTTCTTGTGAAAACAAGC CTCAAAGCCATATGGACACTGTGACAATGACTAAGCCAAGCTGTGTTCATCCAAATACTCAGCTGGCCGG GGAAAGGAGTTCTTTGGCTGGATTGGATTTGTCCAAACAGGTGCTGGCCCAGCATGGAATCCGATGAAAA CCCTCTGATTGGTCTGGGTAGCTGTGAGCAGAAGACGGTTTACCAGGGGCCCAGGAGTATTTGGAAGCAA CGTGTTAATTATAAACAGCAGGGTTTGAGCACAATCTGTTCTACTCTTAATGATGTTATCTTAACACTGA AATTGCCTGAAACCCATTTACTTAGGACTGTGTTTTGCTCTATGAACTATCCCCTGCGCTTTGAACGTGC CAGCAGCCCTTGTTTATTTGCCCAGCCTTTTCACTTCCTCTCCACAGGAGTCTCTGCTGGCTCTTGCCAA AGCAGATTTCCTAAGGCCACTATTTTAAAAGATCATAGTTGCTAAAGTATGTGCTGTGTATGTTTATCTT CTTACCTAGTTTTCTCAAAACCTGGCAGCTTGTTCATTGTGCACACAGCAGCTTCATGTCTAACTCTGCT TGCTAACCTGACAGTACTGGTCACTCCCAGTTCACCAACGTATTGTGTTCCTTCCTTCCATTTTTCTTTT GAGTTGATGAATTTTGAGAGCGCCACAATAAAATTCTTCCTCTTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3601908 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3601908 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10958 same embryo
	EMAGE:10959 same embryo
	EMAGE:10961 same embryo
	EMAGE:10962 same embryo
	EurExpress:euxassay_003489 same experiment
	MGI:4826983 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source