EMAGE mouse gene expression spatial database

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EMAGE:10961

Cdca7l cell division cycle associated 7 like ( MGI:2384982)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003467. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003467_01	euxassay_003467_02	euxassay_003467_03	euxassay_003467_04

euxassay_003467_05	euxassay_003467_06	euxassay_003467_07	euxassay_003467_08	euxassay_003467_09

euxassay_003467_10	euxassay_003467_11	euxassay_003467_12	euxassay_003467_13	euxassay_003467_14

euxassay_003467_15	euxassay_003467_16	euxassay_003467_17	euxassay_003467_18	euxassay_003467_19

euxassay_003467_20	euxassay_003467_21	euxassay_003467_22	euxassay_003467_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10961_wholemount_strong.wlz

10961_wholemount_moderate.wlz

10961_wholemount_weak.wlz

10961_wholemount_possible.wlz

10961_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10961_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 10 11 12 13 14 15

submandibular gland primordium

strong

regional

strong expression: see section 05 06 07 08 09 15 16 17 18 19

pancreas

strong

regional

strong expression: see section 08 09 10 11 12 14 15 16 moderate expression: see section 17

adenohypophysis

strong

regional

strong expression: see section 10 11 12 13

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vibrissa

moderate

regional

moderate expression: see section 02 03 04 05 06 18 19 20 weak expression: see section 21 22

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 08 09 10 13 14 15 16

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20 21

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 15 16 17 18 19 20

midbrain ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 moderate expression: see section 06 16

ventral grey horn

moderate

regional

moderate expression: see section 13

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 20 21 22 23

neural retina

moderate

homogeneous

moderate expression: see section 01 02 21 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 06 07 08 09 10 11 13 14 15 17 18 weak expression: see section 16

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 14 15

esophagus

strong

regional

strong expression: see section 11 12 13

stomach

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 moderate expression: see section 01

hindgut

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18

midgut

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

liver lobe

moderate

regional

moderate expression: see section 01 02 16 weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15

bladder

weak

regional

Expression in the fundus region.

maturing glomerular tuft

strong

regional

strong expression: see section 06 07 08 09 10 16 17 18 19 20 21

testis

moderate

regional

moderate expression: see section 04 05 06 07 20 21 22

urethra of male

moderate

regional

moderate expression: see section 12 13

lung

strong

regional

strong expression: see section 23

left lung

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12

right lung

strong

regional

strong expression: see section 13 14 15 16 17 18 19 20 21 22 23

trachea

moderate

regional

moderate expression: see section 12 13 14

rectum lumen

moderate

regional

moderate expression: see section 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6686
Entity Detected:	Cdca7l, cell division cycle associated 7 like ( MGI:2384982)
Sequence:	sense strand is shown >T6686 CGCTGCGTGCTCGGGGCCGCGCCAGCCCGGGTGGCCGCGTGCAGGGCTCAGGGAAGTGAGCCCAGCAGGG AAGATGGAGCTGGCCACGCGCTCCCAGATCCCCAAAGAAGTAGCTGACATCTTCAGTGCTCCCAGTGACG ATGAAGAGTTTGTGGGTTTCCAGGATGATGTTCCCATGCAAAACCTGTCAGAGAGCTGTGGTAGTTTGGA CTCACGGGAGTTGGAGAAACAGCAGAATGTGTGCTTCCGTTCCAAATACTTCACAGAAGAACTAAGAAGG ATTTTCAAAGAGGACACGGACTCAGAAATGGAAGATTTTGAAGGATTTACAGAGAGCGAGCTGAACATGA GCAGTAACCCAGAGCTCATGGAGTCGGAGCTGAGTGACAGTGACAAAGCATACCCTGTGATGAATGATGC GGAGGAAGATGATGAAGAGGAGGCTGCACCTAGAAGGGGCAGGTCCACAAGAAGAAGCAGTTTCGGTCTT CGGGTAGCCTTTCAGTTCCCCACCAAAAAATTGGCAAGAACACCAGATAAAGATAGCTCACACTTGCTGG ACAGTAAGACGGATCTTAGAAGAAAGAAGAGCTCCAGACAGCCCAAGGGAAAGGAGGACTCTGCTTCTGA TGCCGAGGATGAGTCCAGAGCTGAGAGCCAAGAGAATTCGGATGCTCTGCTGAAAAGGGCCATGAACATC AAAGAGAACAAGGCTATGCTTGCTCAGTTGTTGGCGGAATTGAATTCGGTGCCAGATTTCTTCCCTGTTC GGACACCACCTTCGGCTTCTAGGAGGCGAACACCACGACGGGCCTTCTCAGAGGGACAGATCACACGGCG CATGAACCCAACCCGGAGTGCACGACCCCCTGAAAAGTTTGCTCTGGAGAACTTCACCTTCTCAGCCACC AAGCTTACTGAAGAGCTTTACAGTTTCAGAAGAAGAAAGACAATCAGTGGGGGGAAATGCCAGACGTATA GACGTCATCGGATATCTTCTTTCCGCTCAGTGAAAGACATCACTGAGGAAGACTTGGAAAACATTGCCAT AACTGTGCGTGATAAAGTCTATGATAAAGTGCTTGGTAACACGTGCCATCAGTGCCGGCAGAAGACCATC GATACCAAGACGGTCTGTCGGAACCAGAGCTGTGGTGGTGTGCGGGGACAGTTCTGTGGGCCCTGCCTGC GGAATCGCTATGGGGAGGATGTGCGGACAGCGTTGCTGGATCCGAAGTGGACATGTCCTCCCTGCCGTGG CATCTGCAATTGCAGTTACTGCCGGAGGCGAGATGGCCGCTGTGCTACTGGAATTCTCATTCATCTGGCC AAGTTCTACGGTTATGATAATGTCAAGGAGTACCTGGAGAGCTTACAGAAGCAGCTGTAGAAGACAGTCG AGAGGAGAGGGATCGCTCGAACTTAACCAGGCGGAGTCCCACGAGACGAGTCTGCTCTTTGTGCCAGCCT TAGACGCTTGTTTTCTAGAATTTACTGGTTCTACTGCTATTGTTTCTTAAAAGATGTTTATATGCTTAAA AAGGTTTCTCAGGAAATATAGTAAAGCAGAGAAATCTGTATCCATACCTGCATAGACCTGTATTGTGACA AGTGTCTGCTGAAGAGTTAAAAGCACAGTTTGAGTGGGGCAGAGTTAATGCTCAGGTAAGTTATAAAGAA GACCTTGTCTGACAGCTCTCGCCTTTGATACTCAGTTCCCCCACGGTCACTCCCTTCCTGCAGAGGCTGT TGCTTTGCCTCCTGAGTGACCATAGCTCAAACCTGATAGTTTATCCTGCTCTGGATATTAAATTCTGGGT CATTAGGAATTTATATTGAAGAATGTTACTCTAAACTACCAAGTGCAATTAACCACACACTGTGAGTAAG GTGTCCTTTTTATTAAGAGACAATAGAAACAGACACTGGAGGCCTCACTGTGCTTGCCTCTTCCTTCAGG ACCTGCCTGGAGCCTGCTGCTTCTATTTTTTTAAAAAAAAATTTTTATAGATTATGCTTTTTATAGAACA CTCACTGCATTGTTTCCAAGTCTTAGGGATACAAGATAAGCCATGGTGTTTTGAATGGGTGTTGGGGGAA GCTAAAATCAATAAGTATTCATCCCTTATAGGTGTGTAGTGTTTGGTCCTTGTGTACTGTGATTACTCGC CCTCCTCAAAGAATAATTCTCTAACAAAAGAATGTATACCCCTGTTACTAAAACATCCTTGCTGTGTCTG GCTGAAGCTGGAGGGACGTGAGCTGTAAAACCAAGGCCGTTCATGCTTCCAGCAGTAGAGCCACACCTGC CAGTACCCACTTAGCTATCCTGTCTTTGCTCCTTAGCCTGAACGTCCACACGTAGGTGGGGCCTCTCGCT TTGGTTTTGTAAACAGGGCACTCGTAGGCATGCTTAATTTCTTGTCTGTCCACGGGCACGGCTTTGGCAA AGATGACGGGCATCATGGACGTTAACTCCTTAAGGCGGGCATCAACAAGGGCTCCTGACTGGATGTCCCA CCTGGCACCTGTGGCAGAGACAACAGTTGTGTAACTGGCATGCGGGTTCTGATGCTCTTAATTGTTCCAT GCAATAAATGTTCCACTTTGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3602150 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3602150 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10958 same embryo
	EMAGE:10959 same embryo
	EMAGE:10960 same embryo
	EMAGE:10962 same embryo
	EurExpress:euxassay_003467 same experiment
	MGI:4823759 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source