EMAGE mouse gene expression spatial database

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EMAGE:11028

Mospd1 motile sperm domain containing 1 ( MGI:1917630)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006541. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006541_01	euxassay_006541_02	euxassay_006541_03	euxassay_006541_04

euxassay_006541_05	euxassay_006541_06	euxassay_006541_07	euxassay_006541_08	euxassay_006541_09

euxassay_006541_10	euxassay_006541_11	euxassay_006541_12	euxassay_006541_13	euxassay_006541_14

euxassay_006541_15	euxassay_006541_16	euxassay_006541_17	euxassay_006541_18	euxassay_006541_19

euxassay_006541_20	euxassay_006541_21	euxassay_006541_22	euxassay_006541_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11028_wholemount_strong.wlz

11028_wholemount_moderate.wlz

11028_wholemount_weak.wlz

11028_wholemount_possible.wlz

11028_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11028_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

forearm rest of mesenchyme

strong

regional

strong expression: see section 01 02

upper arm rest of mesenchyme

strong

regional

strong expression: see section 01 02

hand mesenchyme

strong

regional

strong expression: see section 03 04 05 06 07 21 22 23

foot mesenchyme

strong

regional

strong expression: see section 06 07 19 20 21

lower leg rest of mesenchyme

strong

regional

strong expression: see section 01 03 04 05 19 20 21 22 23 moderate expression: see section 02

upper leg rest of mesenchyme

strong

regional

strong expression: see section 03 04 05 06 19 20 21 22 23

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

choroid invagination

strong

regional

strong expression: see section 05 06 07 08 09 10 14 15 16 17 18

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 06 07 16 17

trigeminal v ganglion

strong

homogeneous

strong expression: see section 20 21 moderate expression: see section 05 06 07 08 16 17 18 19

vagus x ganglion

moderate

homogeneous

moderate expression: see section 07 16

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 17

tongue extrinsic muscle

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16

tongue intrinsic muscle

strong

regional

strong expression: see section 09 16

tongue intrinsic muscle transverse component

strong

regional

strong expression: see section 10 11 12 13 14 15

tongue intrinsic muscle vertical component

strong

regional

strong expression: see section 10 11 12 13 14 15

tail paraxial mesenchyme

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6835
Entity Detected:	Mospd1, motile sperm domain containing 1 ( MGI:1917630)
Sequence:	sense strand is shown >T6835 GCAGTCGCAGCTGCTTTCCGAGGAGCCAGTGTTGCTGAGATTGCCAAAATGCTTTGGAGTTTTTAACTGA ATCTAAGAAAGTCCAAACTAGATTTGAGACTGTAAAGCCAGAAGCTGCAGCAAGTGGGATCCAGTGCCAA TGCATCAACAAAAAAGACAACCAGAGTTAGTGGAAGGGAATCTTCCTGTCTTTGTGTTTCCCACGGAGCT AATATTTTATGCAGATGATCAGTCAACACATAAACAAGTGTTGACTCTGTACAATCCCTATGAGTTTGCC TTAAAGTTCAAAGTTTTGTGTACGACTCCAAATAAGTATGTTGTCGTTGATGCTGCCGGTGCAGTGAAGC CACAGTGCTGTGTGGATATTGTGATTCGTCATAGAGATGTTCGATCCTGTCACTATGGTGTGATAGACAA ATTCCGCCTCCAGGTTTCTGAACAAAGCCAGAGGAAGGCCTTGGGAAGGAAAGAGGTTATTGCCACCCTT CTCCCATCTGCTAAAGAACAACAGAAGGAAGAGGAGGAGAAGAGAATAAAGGAACACTTAACCGAGAGTG TGTTCTTTGAGCAGTCATGTCAACCAGAAAACAGAGCCGTCTCTTCAGGACCTAGTTTGCTAACTGTCTT CCTGGGTGTGGTATGTATTGCGGCCCTGATGCTGCCTACTCTAGGGGATATGGAATCGCTGGTGCCTCTC TACCTCCACTTAAGTGTGAATCAAAAACTAGTGGCTGCTTATATCTTAGGTCTTATCACAATGGCTATAC TTAGAACATGAGCAAGGATTGAAACTGACTTCTGAAGCAAATTTATCTTAAAATATGAATGTGGACTGCC TTTTATCTCTTTCACTCCATTAACACACTACAAATTATTGAATGATTTCAAATAACTGCAAATGTATAAT ATATATTTTCAATTAGTCTATAATTTTATTTGAAGGACTGTATCACTTAATGTTACAGACAACAAAGATA CTTCTGAGTGACACCTAGGAAATTATTTGAAGACATTCTTTTTAAATCTATACCTGTTGTGCAAATAACT TCAACGTTATTGTTCATCTTTTTTATTTTGAACTCACTTTGATAAGGGTCATGGGTCCTTCAAAGTTTTA GACCTAAATGAACAACTGACCAGCTTGAAAATATAAAATGACATTTATTGCCTAGACGCAAGCACCAACA TTACTATGTAAGTCATAGATTTCCTCATCATTACAAGGAGCTGCTGTGGTGTTTCTAAGGGGAAACAGTC AGTGTGTGAGGGGTTGAAAACATAAGGTACTTACTGAGCGATCATGCTTACCCTGGACTCTTCAAAGAAG ACAAGATGTATTGAAATGAGCTTGTGAACTACACAACAGACTGTGGTTCAAACAACAGGCACCTCACAGA AGTAGATTGGTTTTTCTGTTTCTAAAGAAAGGTAGGCATTTTTTTTAATTAATTGGTCCATCCTGGCTTT ACTTCTGGTTAATCCTTGATGTATTAAGGAGAAAATCACTTTTTGGGTTTCTCCCAGGTCCCTTGCTTGC CTGAGTGTAAGTGACTTGCCCTGGTTAGGAATGTCAGATGACAATTAGTTTCTGAGGACATGAATTTTTT TACATTCAAACTCTTAAGCCAAGTAGAGAAAGCCCTAGATGCCCGTGTCTATTTTGTATTGGCCACTGGG ATGTTTTTTAAGTTTTTATTTATTCAAATAAATTTACCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3962906 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3962906 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11027 same embryo
	EurExpress:euxassay_006541 same experiment
	MGI:4826423 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source