EMAGE mouse gene expression spatial database

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EMAGE:11032

Apln apelin ( MGI:1353624)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003400. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003400_01	euxassay_003400_02	euxassay_003400_03	euxassay_003400_04

euxassay_003400_05	euxassay_003400_06	euxassay_003400_07	euxassay_003400_08	euxassay_003400_09

euxassay_003400_10	euxassay_003400_11	euxassay_003400_12	euxassay_003400_13	euxassay_003400_14

euxassay_003400_15	euxassay_003400_16	euxassay_003400_17	euxassay_003400_18	euxassay_003400_19

euxassay_003400_20	euxassay_003400_21	euxassay_003400_22	euxassay_003400_23	euxassay_003400_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11032_wholemount_strong.wlz

11032_wholemount_moderate.wlz

11032_wholemount_weak.wlz

11032_wholemount_possible.wlz

11032_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11032_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pericardium

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

axial musculature

moderate

regional

moderate expression: see section 06 07 19 21 22 weak expression: see section 03 04 05 20

pancreas

moderate

regional

moderate expression: see section 09 10 11

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tongue muscle

moderate

regional

moderate expression: see section 10 11 15 16 weak expression: see section 12 13 14

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 weak expression: see section 08 09

hindgut

weak

regional

weak expression: see section 12 13 14 15 16 17

midgut

weak

regional

weak expression: see section 10 11 12 13 14 15 16 17 18 19 20

metanephros

moderate

regional

moderate expression: see section 08 18 weak expression: see section 07 09 10 17 19 20

genital tubercle of male

moderate

regional

moderate expression: see section 13 14 15 17 weak expression: see section 16

testis

weak

regional

weak expression: see section 05 06 07 08 20 21 22

lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22 23 24 weak expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5944
Entity Detected:	Apln, apelin ( MGI:1353624)
Sequence:	sense strand is shown >T5944 GCCTGGACCAACGGGTAGTTGAGAGTCGCGGGCAGAGCAGCGTCAGCCACTTAGAGAGTTTTTGCCGCCG ACCCGAAGCCACCAAGGCCAGCTTCGCGGCGCTGCCCCGCGGCGGCAGAGAAGGCTGCACCAGAGCAGAG GCAGCGAGCAGGAGTGGGGCAGGCAGCCAGCGGTGCGGCTGGGGCGCTCACCCTCCCGCGGTCCGGGAGC CACGCGAGCTCCGTGCCCGCACGCGCCAGCCCCGGCTCGCGCCTTTCTTTGCGTCCGGGTGCCCTGCCTC TCCGCCCACTCGCCGGCTCCTCTGGGCTGCCGCGGACCGAGTTGCAGCATGAATCTGAGGCTCTGCGTGC AGGCGCTGCTGCTGCTCTGGCTCTCCTTGACTGCAGTTTGTGGAGTGCCACTGATGTTGCCTCCAGATGG AACAGGACTAGAAGAAGGAAGCATGCGCTACCTGGTGAAGCCCAGAACTTCGAGGACTGGACCAGGAGCC TGGCAGGGAGGCAGGAGGAAATTTCGCAGACAGCGCCCCCGGCTCTCCCATAAGGGCCCCATGCCTTTCT AAAGCAGGATTGAAGGGCTCGCCAAGTGCCCTCCCGGTGCCGGTCTCTCTACTCCACAGATGAATTCTCT TCTCTGGAACCCTCACATCTATTTGGCTTTCATCTTGCACCTGTTCTAGCTGCTGATGGTCCCGGCTCTT CTCACCCACCAAGTTCCTCTAATGGCGTGGTCTCGCCCCTCACTTGGATGATTTCATTCCTCTCACAAGG ATCCCTTTGGCCCATCCTCCTGGCCACTCTTTGGACTGCCCTTGGAGACCCAGCTTCTCCTTGACCTGTT CCATGCGGTAGTCCCTATCTTTCAGCCGTAGCCTTCTCTTGGCTCTTCCCTCTTTTCATTTGCCTTGGGC ACTGGACAAGAACACTCCCTTCTCCAGTGGATTCCAGCACATCCATGCTTTCTTATCAATTGGACCTCTT GCCTGGAAGGGAGCGCCGAGATGTGCAATGAGACTTCGGTTGAGAGGGGTTGGGTGTTGGGGTCCAGATA AGGTGCACTGGGGAAACTGAATGGTGGGCGTGAGGAAGGGGTGTGGCGAAATAGCTGCCACAGCCTCTTC CTTAGCTCTCTCTGGCCCTGTTAAAGGAAGACGCAAAGGAAAGGAGCCCATGAAGTTCTATGTCACCGTG CCTTCTAATGTCTTCGCTCTCTCTGTTCTATTGCAGCTTGTTCAGTCCCCAGGTCAGAAGGGGAGAGTGT GGATCCTGACATGGTTCTATACTCTTCTGGGGGTAATTTTGCCAAAAAAATAATCCCAACCAATCAACCA ACCAACCAAATAACCCACAGAAAGTCAGAGCCCAAAGGACCCTTGAAGATCACTTAGCTCCCTTATGCAA AAGCTTGCACAAATAGGCCCTGGAGAGGGAAGTAACCTGCTTAGAGCCACTGCAGAGCCCCACAGGGCAC CAGGGCCCTCAACTCCCACTTTATGATGCCCTTTTTCCTCTTGATGATGTCCTTTCAAAGCAAATGAAGT GCCTTTTGCTGGGTTTAGTGAAGCGGGTGGCTAGGAAGGAAGAGCAGGGAGAGACAAGCCAGCTGTGAAC TGCTTTGTGTGAGAATGGAGCATCTCCCTCCCAACGTGGCCCGGAATAGTCCCTAAGTGGTGAAATGTAC CCCACACAGGCCTATTCCCAGGCTCATGCCATTTTAATTGGGGTTCTCAGGCACTGAAACTACCATTTAG CCCCTGACACTGGTTGTCATGAACCCCTGTGTTGACTGCGCCCTTGATCTTGGGGCTTTTGGGGGATGGA GAAAGGCAAAGAAAGGAAGGAGAGGTGGAAGAAGGAAGGAGAGATGGGAGGATGTGTAGGTGGGATCTAT CACTCCACCAAGGGGGTCTCATCTCTCACCAGGAGCCTTTTGTAGCCCTTCCCTGAAGGGAGACCCTCAT CTTCTCTGCCTGAGCACATGTTCTTCTCAGAGGCCTCTCAGGAGGGAGAAAAGAGAAGACAGAAGCTCGA TATTCACTTATATATGTGTAGGGTAGGGGGAGTCAGGACCCCCAAGCCCCATAATAACCTGATTGCCTAT TCATCCTTTCCCAAAGCCTTCCTATCTGCTGCTGCTAATGCTGCTGCTGCTGCCTCTGCTGCCGTTTCAA GGCCCACCCTGGGGAGCAGGGCCAGGCATAGCGTCCTCACCTCTTGGGCTAGAAGTGGGAGCTGGCTGGC GCCTCTTCTACTGCTGGTACCCCTACCAGGGACCGGGCCTTCTCCGTCTTTGTCGTTTCTGCACCTCCTG CCTTATCAGCCTGCCATGGCCAGCCCCACAGGGAAAGGGAGGGGGGAATATTGGCTGAGAAAGAGGAGAG ATAGAAGCACAAGAGGGGAGTAAACGGGCCCAGTGGGCTGTCTTATTTAAAGTGGTTATGTGTATTTCTT ATACTAATTTATATAGAGATATTAAGGCCCTTTGAGTTAGAGAAACTGTCCCCTTCCCATAAGTGTGTTC GCTATGTTTGTAAAAATTGTTCCATGTAAATATGTCTTTATAATAAAGAGTTAAAAGTTGACAGTTTGCC CTTACTCTTTGAGGTCATATTCAGGAGGGCATTCTTTTTCTCCTAGGGCCAATCTTGTCCCTGTGCCCAC AGATTGCACGTGTAGGGAGGTGAGTGCTTGTATCCCAAATTGGTTCTAGGTCAACTGGCCTCAAACTGAT TTGCCACAAGCTCACAAAAAGACTCGCTATGCTTAAGGGCCAGTTCACATAAATTTGAGCCCCTTTTAAG TCCTTTGGCATCTGTTCGAATGTCCTCATTTTTCTGGCAGTGTCACTTAAAGGATTTTTTTTCCAGAAGC AATTCATTTAAGGACACGCTGATCAAAGGTGGGATTTGTACTAAACGCTGTTTTGTAGCCCTGGTGGGTG CCTTCTGTTTTATCTGCTTTTGAATACTTTCGGGACTTTTTAGCCAGTTTGCCTTTCTTGACAAATGTTG TCCTCAGCCATAAATACATTTGGTAATGACTTTGTATGTATCGTTTTATTGTTTCACAAAGTGGAGTTGC TTGATGAATGAGATAACCTGGAAAATAAACTTCGAGGAGTTCGATAGGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3483588 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3483588 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11030 same embryo
	EMAGE:11034 same embryo
	EMAGE:11033 same embryo
	EMAGE:11029 same embryo
	EMAGE:11031 same embryo
	EurExpress:euxassay_003400 same experiment
	MGI:4823178 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source