EMAGE mouse gene expression spatial database

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EMAGE:11036

Rpp30 ribonuclease P/MRP 30 subunit (human) ( MGI:1859683)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006550. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006550_01	euxassay_006550_02	euxassay_006550_03	euxassay_006550_04

euxassay_006550_05	euxassay_006550_06	euxassay_006550_07	euxassay_006550_08	euxassay_006550_09

euxassay_006550_10	euxassay_006550_11	euxassay_006550_12	euxassay_006550_13	euxassay_006550_14

euxassay_006550_15	euxassay_006550_16	euxassay_006550_17	euxassay_006550_18	euxassay_006550_19

euxassay_006550_20	euxassay_006550_21	euxassay_006550_22	euxassay_006550_23	euxassay_006550_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11036_wholemount_strong.wlz

11036_wholemount_moderate.wlz

11036_wholemount_weak.wlz

11036_wholemount_possible.wlz

11036_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11036_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 13 14 15 16 17

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 10 11 17 18 19 20

pancreas

moderate

regional

moderate expression: see section 09 10 11 12 15 16

thyroid gland

moderate

regional

moderate expression: see section 13 17

vibrissa

moderate

regional

moderate expression: see section 05 06 07 08 19 20 21 22

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13 14 15

telencephalon ventricular layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

midbrain ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 19

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 12 14 15 16 17 18

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

metanephros

moderate

regional

moderate expression: see section 08 09 10 11 12 17 18 19 20 21

seminiferous cord

moderate

regional

moderate expression: see section 08 09 21 22

lung

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7943
Entity Detected:	Rpp30, ribonuclease P/MRP 30 subunit (human) ( MGI:1859683)
Sequence:	sense strand is shown >T7943 GTCAGCATGGCGGCGTTCGCAGATTTGGATTTAAGAGCGGGCTCAGACCTGAAGGCTTTGCGCGGACTCG TGGAGACTGCTGCTCACCTGGGATACTCAGTTGTTGCCATCAATCACATTGTTGACTTTAAGGAAAAGAA ACGGGAAATTGAAAAGCCAATAACAGTTTCTGAACTCTTCACAACTTTACCCATCGTACAGGGAAAATCA AGACCAATTAAAATTTTAACTAGATTGACAATTATAGTTACAGATCCAGCTCACTGTAATGTTTTGAGAG CAACTTCGTCAAGGGTCCGGCTGTATGATATTGTTGCAGTCTTTCCAAAGACAGAAAAGCTTTTCCATGT TGCTTGTACACATTTAGATGTGGATTTAGTCTGTATAACTGTAACAGAGAAATTACCATTTTACTTCAAA AGACCCCCTGTTAATGTGGCAATTGAGCGAGGCCTGGGCTTTGAACTTGTCTATGGTCCTGCTATCAGAG ATGCAACGATGAGAAGGTACACAATCTCCAATGCCCTCAATTTGATGCAGATCTGCAAAGGAAAGAATGT AATTCTGTCCAGTGCTGCAGAAAGGCCTTTGGAAATAAGAGGACCATATGACGTGGCAAACTTAGGACTG CTGTTTGGGCTTTCTGAAAATGATGGCAAGGCTGCCGTGTCCACAAATTGCAGAGCTGTATTTCTCCATG GAGAAACTAGAAAAACTGCTTTTGGAATTATTTCTACAGTGAAGAAACCACGGCCATCAGAAGCAGATGA TGAATCTCTTCCAGTGTGCAAGAAAGCTAAATGTGAGGGCTGAGACGAGTCCTGAGTCTCCACCCGCTGC CTCCTCCCCTTCATAGCTCATCAGTCACTGCAAAAATAAAACTCTGCTGAGTTGATATTTTCATTAAACT AGAAACCAACAGTCTATAAAAACAACTTTATATTCAGACCATTAAAACAGCAAATAAAACCCAGTGAAGC ATTTTTTAAAGACTAACAGCTTAATTAATTTAGATATACTTTAAGAGAAAAACTATTTTATCCTTCATGT GAATAATGAATAACAGTAGGTACACTGAAATAAGATGTCTCAGTATTGTAAGACATTGAAAACTAATAAA TTAACGTTTTTCTACTTGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6518236 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6518236 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11037 same embryo
	EMAGE:11035 same embryo
	EurExpress:euxassay_006550 same experiment
	MGI:4827804 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source