EMAGE mouse gene expression spatial database

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EMAGE:11039

Tmem35 transmembrane protein 35 ( MGI:1914814)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003210. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003210_01	euxassay_003210_02	euxassay_003210_03	euxassay_003210_04

euxassay_003210_05	euxassay_003210_06	euxassay_003210_07	euxassay_003210_08	euxassay_003210_09

euxassay_003210_10	euxassay_003210_11	euxassay_003210_12	euxassay_003210_13	euxassay_003210_14

euxassay_003210_15	euxassay_003210_16	euxassay_003210_17	euxassay_003210_18	euxassay_003210_19

euxassay_003210_20	euxassay_003210_21	euxassay_003210_22	euxassay_003210_23	euxassay_003210_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11039_wholemount_strong.wlz

11039_wholemount_moderate.wlz

11039_wholemount_weak.wlz

11039_wholemount_possible.wlz

11039_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11039_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

strong

homogeneous

strong expression: see section 09 10 11 12 18 19

diencephalon

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

telencephalon marginal layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 11 12 13 14 16 17 18

hindbrain

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

facial vii ganglion

weak

regional

weak expression: see section 21

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 08 18

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 10 18 19 20 21 22

vagus x ganglion

weak

regional

weak expression: see section 09

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 07 08 09 18 19

spinal cord

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 15 16 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 17

cervical ganglion

moderate

regional

moderate expression: see section 10

thoracic ganglion

moderate

regional

moderate expression: see section 15 16

dorsal root ganglion

weak

regional

weak expression: see section 08 09 10 11 12 13 17 18

not examined

regional

not examined expression: see section 02 03 04 22 23 24

neural retina

moderate

regional

moderate expression: see section 02 03 04 23 24 weak expression: see section 01 05 22

maturing glomerular tuft

weak

regional

weak expression: see section 09 10 11 12 18 19 20 21

testis

weak

regional

weak expression: see section 07 08 09 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5443
Entity Detected:	Tmem35, transmembrane protein 35 ( MGI:1914814)
Sequence:	sense strand is shown >T5443 GTTGGAAGTTGCCTTCTCACTTCAGGGCTTCTTTTAACTTTGGCAAGGCACCGTCCTTTACGCAGGGAGG TGCAAGCGTAGGGCATTAGGACCCAGCGATTGTTGTAGTGAGAGTCGTTGAATCTCGATCTCTCAATTTT TCTCTCTAGCTCCCTCCTCTCCCTTTGTCATTCTCTCTGCTGCCTCCGCAGCGTGCCTCTAGCTCTCCCT GTGCTAACTGCCTGCGCCTTGACAGCCGGGCTGCGCAAAGCAGAGGGATTAGTTGGGACCTGCCTTGGTG ACCCCATGGCATCCCCACGAACCATAACTATCATGGCCCTCTCAGTGGCCCTGGGACTCTTCTTTGTTTT CATGGGGACCATCAAGTTGACCCCCAGGCTCAGCAAGGATGCCTACAGTGAAATGAAACGTGCTTACAAG AGCTACGTTCGAGCCCTTCCTCTGCTGAAGAAAATGGGCATCAATTCCATCCTCCTTCGGAAAAGTATTG GTGCTCTTGAAGTGGCCTGTGGCATTGTCATGACCCTGGTACCCGGTCGTCCCAAAGATGTGGCCAACTT CTTCCTGCTCTTGCTGGTGTTGGCTGTGCTCTTCTTCCACCAGCTGGTGGGTGATCCTCTCAAACGCTAC GCCCATGCTCTGGTGTTTGGAATCCTGCTCACCTGCCGCCTGCTGATTGCCCGCAAGCCTGAAGACAGAT CTTCTGAGAAGAAGGCCTTGCCTGAGAGTGCAGAGGAGCAACCATCCTTGTATGAGAAGGCCCCCCAGGG CAAAGTGAAGGTGTCATAAAAATTGGAAGTACAAAGAATGGACCTTCTAGGCGGTTGCTTCCTTGACACC CAAGATGACATCAGTGTGTGTGCGTGTGTGTGTGTGTTTTCCTTTGATTGTTTATCTTGGGAGTAAAGAG AAAAATACAATCTGTAAGTTAATTGGCTTGTGTACATCTATACCCTAAAATGACCTCCCCACATTGACAT TCGTGTACCACCTTTAATTCCTCTGGAGCAACACTCACATCTTGCTGCATGTACATGTATATGGCTACTA TTGAGTTGTATTTGTGAGATGGACTCCAGCAAGCATGTGACTGTGAGATTATGTGTGGGGCAATGTATTT AACTGCCGTGTGTGGGTATACACGTACTTGTAGAGGAGGCTCTGATCTTGAACTAATCCTGTGCAAGTGT CCTTCCCTTTATAAATTGATTCCAGATAAGAACTGTCTCCTAAAGAGAGTTGTTTCCTGGTGGAAAACAA ATGACCTAGTTTGATCCTTCAAGTGATGCAGTGGGAGGGGTCGCCTTGCCTTTGTCTGAGTACAAGCTAT TGAGCTCTTTAGTAACCTTTTACTAGACACAGTCAACTTTTGTTTTAAGCACAAAGCAGCCCCACTTTAC CATTCTCAGTAGCCACCCAGGAGCTGAAGTTTCCCCACTGCTTCCACTGAGGGACCAGGGATTGGTCCTG GTACAAGTTTCAATCTCCAACCCTAGTGGTTTTCTGAAACCTGCAACTGGATGGAGAAGCTGTAGGCAGT ATTTATCTGTATGAAGAGTTCTCTACACCTATTGATTCCCCACCCAACTTTGCTAAAGTTAACAACTCCA AAACACCCCCTAGAAATGTCTACTTTACACCTCTTCAGCGTTCCAGAGGGTGGAGTTTAGCTGGGGGAGG GACATTTGACTTGGGTTAGTTGGATTGACCAGTATGGAAATTTGCTTTTGTAGTTACATACTGCTGTTTC TCTTTGTTAAATGTGCTTTGATGGTTTTAATATTGTTGTGATGGGGAAGGGGGTGGGTTTGTGTCTAGGG AGAGTGCTTACTGTATTTCTTCAGTGCCATTGTTCTTGGTAATTGATACTTGTCTTCTTTTGCTCATTCT TTCAAAATAAAGATTTTTGAAATTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5369317 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5369317 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11041 same embryo
	EMAGE:11040 same embryo
	EMAGE:11038 same embryo
	EMAGE:11042 same embryo
	EurExpress:euxassay_003210 same experiment
	MGI:4828801 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source