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EMAGE:11040

Mtmr7 myotubularin related protein 7 ( MGI:1891693)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11040 EMAGE:11040 EMAGE:11040 EMAGE:11040 EMAGE:11040
"Pseudo-wholemount" of euxassay_003206. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003206_01 euxassay_003206_02 euxassay_003206_03 euxassay_003206_04
EMAGE:11040 EMAGE:11040 EMAGE:11040 EMAGE:11040 EMAGE:11040
euxassay_003206_05 euxassay_003206_06 euxassay_003206_07 euxassay_003206_08 euxassay_003206_09
EMAGE:11040 EMAGE:11040 EMAGE:11040 EMAGE:11040 EMAGE:11040
euxassay_003206_10 euxassay_003206_11 euxassay_003206_12 euxassay_003206_13 euxassay_003206_14
EMAGE:11040 EMAGE:11040 EMAGE:11040 EMAGE:11040 EMAGE:11040
euxassay_003206_15 euxassay_003206_16 euxassay_003206_17 euxassay_003206_18 euxassay_003206_19
EMAGE:11040 EMAGE:11040 EMAGE:11040 EMAGE:11040 EMAGE:11040
euxassay_003206_20 euxassay_003206_21 euxassay_003206_22 euxassay_003206_23 euxassay_003206_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11040Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11040_wholemount_strong.wlz
11040_wholemount_moderate.wlz
11040_wholemount_weak.wlz
11040_wholemount_possible.wlz
11040_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11040_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
foregut-midgut junction
moderate moderate
regionalmoderate expression: see section 16 17 weak expression: see section 13 14 15
pancreas
weak weak
regionalweak expression: see section 11 12 13
diencephalon
moderate moderate
homogeneousmoderate expression: see section 08 09 10 11 12 13 14 15 16 17 18
pituitary gland
moderate moderate
regionalmoderate expression: see section 17 weak expression: see section 12 13 14 15 16
vibrissa
strong strong
regionalstrong expression: see section 08 09 10 19 20 moderate expression: see section 07 21 weak expression: see section 05 06 22 23
telencephalon mantle layer
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
telencephalon marginal layer
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22
olfactory cortex marginal layer
moderate moderate
regionalmoderate expression: see section 11 12 13 14 17 18
hindbrain
moderate moderate
homogeneousmoderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
midbrain
moderate moderate
homogeneousmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19
facial vii ganglion
strong strong
regionalstrong expression: see section 06 20 21 22
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 07 08 09 18 19
trigeminal v ganglion
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 18 19 20 21 22
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 07 08 09 18 19
spinal cord
moderate moderate
homogeneousmoderate expression: see section 10 11 12 13 14 15 16 17
cervico-thoracic ganglion
moderate moderate
regionalmoderate expression: see section 11 12 17
cervical ganglion
strong strong
regionalstrong expression: see section 18 moderate expression: see section 10
thoracic ganglion
moderate moderate
regionalmoderate expression: see section 15 16
dorsal root ganglion
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 17 18
cornea stroma
strong strong
regionalstrong expression: see section 01 moderate expression: see section 02 03 04 05 23 24
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 12 13 14 16 17 moderate expression: see section 09 10 11 18 19
stomach
weak weak
regionalweak expression: see section 05 06 07 08 09 10 11 12 13
hindgut
moderate moderate
regionalmoderate expression: see section 16 17 weak expression: see section 13 14 15
rectum
moderate moderate
regionalmoderate expression: see section 16 17 weak expression: see section 15
midgut
moderate moderate
regionalmoderate expression: see section 16 17 18 19 20 weak expression: see section 10 11 12 13 14 15
lower jaw incisor
strong strong
regionalstrong expression: see section 12 13 16 17
lower jaw molar
moderate moderate
regionalmoderate expression: see section 08 19
upper jaw incisor
strong strong
regionalstrong expression: see section 12 13 14 17
upper jaw molar
moderate moderate
regionalmoderate expression: see section 08 19
lung
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 13 17 18 19 20 21 22 23 24 weak expression: see section 10 11 12 14 15 16
nucleus pulposus
strong strong
regionalstrong expression: see section 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5437
Entity Detected:Mtmr7, myotubularin related protein 7 ( MGI:1891693)
Sequence:sense strand is shown

>T5437
CCACGCGTCCGCACGCGCGCGCGCAGGTGCGTGAGGCCGCGCCCGCCGGGGACCCTGCAGACGCGGGCCC
GCCATGGAGCACATCCGCACGCCCAAGGTTGAAAACGTGCGCTTGGTAGACAGAGTGTCTTGTAAAAAGG
CAGCGCTGGGAACTTTGTATCTGACGGCCACTCACGTCATATTCGTGGAAAATGCACCTGACACAAGAAA
AGAAACCTGGATTCTGCACAGTCAGATCTCCACCATTGAAAAGCAGGCGACAACAGCTACGGGCTGCCCT
CTGCTTATTCGCTGCAAGAACTTCCAGATCGTACAACTTGTCATTCCACAGGAGAGAGACTGCCACGACG
TGTACATCTCTCTGATACGCCTTGCACGGCCAGTGAAGTACGAGGAGCTATACTGCTTTTCCTTCAACCC
CAAGCTGGATAAGGAAGAACGGGAGCAGGGCTGGTTGCTGGTTGACCTTAGTGAAGAATACAAGCGAATG
GGCCTCCCTGATAATTATTGGCAGCTCAGCGATGTGAATAGAGACTACAGAGTTTGTGACTCTTACCCCA
CGGAACTGTACGTCCCCAGATCGGCCACGGCACACATCATCGTGGGCAGCTCCAAGTTCCGGAGCAGACG
GCGATTTCCTGCCCTTTCCTACTACTGTAAAGACAGCCATGCCTCCATCTGCAGGAGCAGCCAGCCCCTT
TCCGGCTTCAGCGCTCGGTGCCTGGAGGATGAGCAGATGCTCCAAGCCATCAGGAAAGCCAATCCTGGAA
GTGACTTCATTTATGTCGTGGACACAAGGCCTAAACTTAATGCGATGGCAAATCGTGCTGCAGGGAAAGG
CTATGAGAATGAAGACAATTATTCCAATATCAAGTTTCAGTTTATCGGCATAGAGAACATCCACGTCATG
AGGAACAGTCTTCAGAAAATGCTGGAAGTGTGTGAACTTAAATCTCCCTCTATGAGTGATTTCCTGTGGG
GACTGGAGAACTCAGGCTGGCTGAGGCACATCAAAGCCATCATGGATGCAGGGATCTTCATTGCAAAGGC
AGTTTCAGAGGAAGGGGCAAGTGTGCTTGTTCACTGTTCTGACGGCTGGGACAGAACGGCCCAGGTGTGC
TCGGTGGCTAGCCTGCTGCTGGACCCTTACTACCGGACTCTGAAAGGATTCATGGTATTAATTGAAAAGG
ACTGGATTTCCTTCGGACATAAATTTAATCATAGATATGGCAACTTAGATGGTGACCCAAAAGAAATCTC
TCCAGTAATCGACCAGTTCATTGAGTGTGTCTGGCAGCTAACGGAACAGTTCCCTTGCGCCTTTGAGTTC
AACGAGAGGTTTCTGACTCACATTCAGCATCATGTTTATTCCTGCCAGTTTGGGAACTTCCTGTGTAATA
GCCAGAAGGAGAGGCGAGAACTCAATGAGAATGGAAAAAGCAAAAAGGGTTCACGGTTCCTTAAGGAGGA
CTCCTGAGGTTGAAGGCTAGCCTGTGTTGAAACCACAGCATACGATTCAAGAAAGAACCTACTCTTTGTG
GTCCAACCTGTGGAAGAATCGAGCTGACTACCTCAATCCTCTGTTTAGAGCTGACCACAGTCAGACCCAG
GGGAGCCTTCATCTGCCTACAGCTCCATGCAACTTCACCTATAATAATAATATAAACTTACTCTTCCTAA
GAGAAGTTAACTACCTTAGAAAAAATATCAACCTGAAATTATTACTTTTAGTAGTTGCTTTTTCAAGGCT
GTAACTTGCTTTTGGTTATTAATATGTTGGGCTGTGATACTGAAGACAAGACTTGGGATTGTGGGCTTTC
AGACTGGGATATGTGCTATCCTTCCTTCCTTCCTCCAGGTTTTGGAATGGGATGTATAACCGCTTTGAAA
AGGGGTTGCAGCCTCGACAGTCTGTTACAGATTACCTCATGGCTGTGAAGGAGGAGTCCCAGCAGCTGGA
GGAAGAACTGGAGTCCCTAGAAGAAAGGCTGGAAAAAATTCAGAAAGTCCACTTACATGGTACCAAAGTG
AAGAGTAAGCAAAGTGAGCCCAGCAAACACTCGGGGTTCTCTACCTCAGACCACAGCACAGCCAACACCC
CTCAGGATTACAGTGGGAACAGCAAGTCCTTCCCATCCAGGAGCCCGTCCCAGGGTGATGAAGATTCTGC
TCTGATTCTAACCCAAGACAATTTAAAAAGCTCAGACCCCGACCTCTCAGTGAATAGTGATCAAGAGTCT
GGGGTGGAGGATTTGAGCTGTCGATCTCCCAGTGGTGGTGAGCATGCACCCAGCGAAGACAGTGGCAAGG
ACCGGGACTCGGACGAAGCTGTGTTTCTCACTGCCTAAGTTCCCCTTTGGAGTTCCAAAGTAAAAGACAC
AGAAACACTTTCAAATTAAGGGGAAAGTGCAATTCGTAGTAAAAATGTTGAGAAATGGCAAGTTCTATCC
AGAACCATAAAATTACAGCTGTGAAAATGGGGAAAAAAAAGCTCTTTATCAATAACCAGTTGTTTTATCA
AATCATACTGAAGACTGATCATCATTCCTGAGGCTCATGCTAGACTATGAAAATTATATCACTACAAGGA
GAGAGAGTATCTTCACCCAAGGTTGTCCAGACAGTCTTTCCTGGCATAGCATGTGTCTGCTTAAAAGCCC
TCCTGGTTTCTCCCATACCTGATAATGTTGGGACTTTGATTTCACTTCCAGTTGCAGGTTGCATTCAGGA
ACTTTGACATTTCCTTCTGCCTTTACCAATATTTAAAAGAAATAAATATGGAGAGTACCCTTTTCTGTTA
TAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5368816 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5368816 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11041 same embryo
 EMAGE:11039 same embryo
 EMAGE:11038 same embryo
 EMAGE:11042 same embryo
 EurExpress:euxassay_003206 same experiment
 MGI:4826498 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS