EMAGE mouse gene expression spatial database

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EMAGE:11040

Mtmr7 myotubularin related protein 7 ( MGI:1891693)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003206. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003206_01	euxassay_003206_02	euxassay_003206_03	euxassay_003206_04

euxassay_003206_05	euxassay_003206_06	euxassay_003206_07	euxassay_003206_08	euxassay_003206_09

euxassay_003206_10	euxassay_003206_11	euxassay_003206_12	euxassay_003206_13	euxassay_003206_14

euxassay_003206_15	euxassay_003206_16	euxassay_003206_17	euxassay_003206_18	euxassay_003206_19

euxassay_003206_20	euxassay_003206_21	euxassay_003206_22	euxassay_003206_23	euxassay_003206_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11040_wholemount_strong.wlz

11040_wholemount_moderate.wlz

11040_wholemount_weak.wlz

11040_wholemount_possible.wlz

11040_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11040_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

moderate

regional

moderate expression: see section 16 17 weak expression: see section 13 14 15

pancreas

weak

regional

weak expression: see section 11 12 13

diencephalon

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

pituitary gland

moderate

regional

moderate expression: see section 17 weak expression: see section 12 13 14 15 16

vibrissa

strong

regional

strong expression: see section 08 09 10 19 20 moderate expression: see section 07 21 weak expression: see section 05 06 22 23

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

telencephalon marginal layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 11 12 13 14 17 18

hindbrain

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

facial vii ganglion

strong

regional

strong expression: see section 06 20 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 09 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 18 19 20 21 22

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 09 18 19

spinal cord

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 15 16 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 12 17

cervical ganglion

strong

regional

strong expression: see section 18 moderate expression: see section 10

thoracic ganglion

moderate

regional

moderate expression: see section 15 16

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 12 13 14 17 18

cornea stroma

strong

regional

strong expression: see section 01 moderate expression: see section 02 03 04 05 23 24

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 12 13 14 16 17 moderate expression: see section 09 10 11 18 19

stomach

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13

hindgut

moderate

regional

moderate expression: see section 16 17 weak expression: see section 13 14 15

rectum

moderate

regional

moderate expression: see section 16 17 weak expression: see section 15

midgut

moderate

regional

moderate expression: see section 16 17 18 19 20 weak expression: see section 10 11 12 13 14 15

lower jaw incisor

strong

regional

strong expression: see section 12 13 16 17

lower jaw molar

moderate

regional

moderate expression: see section 08 19

upper jaw incisor

strong

regional

strong expression: see section 12 13 14 17

upper jaw molar

moderate

regional

moderate expression: see section 08 19

lung

moderate

regional

moderate expression: see section 05 06 07 08 09 13 17 18 19 20 21 22 23 24 weak expression: see section 10 11 12 14 15 16

nucleus pulposus

strong

regional

strong expression: see section 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5437
Entity Detected:	Mtmr7, myotubularin related protein 7 ( MGI:1891693)
Sequence:	sense strand is shown >T5437 CCACGCGTCCGCACGCGCGCGCGCAGGTGCGTGAGGCCGCGCCCGCCGGGGACCCTGCAGACGCGGGCCC GCCATGGAGCACATCCGCACGCCCAAGGTTGAAAACGTGCGCTTGGTAGACAGAGTGTCTTGTAAAAAGG CAGCGCTGGGAACTTTGTATCTGACGGCCACTCACGTCATATTCGTGGAAAATGCACCTGACACAAGAAA AGAAACCTGGATTCTGCACAGTCAGATCTCCACCATTGAAAAGCAGGCGACAACAGCTACGGGCTGCCCT CTGCTTATTCGCTGCAAGAACTTCCAGATCGTACAACTTGTCATTCCACAGGAGAGAGACTGCCACGACG TGTACATCTCTCTGATACGCCTTGCACGGCCAGTGAAGTACGAGGAGCTATACTGCTTTTCCTTCAACCC CAAGCTGGATAAGGAAGAACGGGAGCAGGGCTGGTTGCTGGTTGACCTTAGTGAAGAATACAAGCGAATG GGCCTCCCTGATAATTATTGGCAGCTCAGCGATGTGAATAGAGACTACAGAGTTTGTGACTCTTACCCCA CGGAACTGTACGTCCCCAGATCGGCCACGGCACACATCATCGTGGGCAGCTCCAAGTTCCGGAGCAGACG GCGATTTCCTGCCCTTTCCTACTACTGTAAAGACAGCCATGCCTCCATCTGCAGGAGCAGCCAGCCCCTT TCCGGCTTCAGCGCTCGGTGCCTGGAGGATGAGCAGATGCTCCAAGCCATCAGGAAAGCCAATCCTGGAA GTGACTTCATTTATGTCGTGGACACAAGGCCTAAACTTAATGCGATGGCAAATCGTGCTGCAGGGAAAGG CTATGAGAATGAAGACAATTATTCCAATATCAAGTTTCAGTTTATCGGCATAGAGAACATCCACGTCATG AGGAACAGTCTTCAGAAAATGCTGGAAGTGTGTGAACTTAAATCTCCCTCTATGAGTGATTTCCTGTGGG GACTGGAGAACTCAGGCTGGCTGAGGCACATCAAAGCCATCATGGATGCAGGGATCTTCATTGCAAAGGC AGTTTCAGAGGAAGGGGCAAGTGTGCTTGTTCACTGTTCTGACGGCTGGGACAGAACGGCCCAGGTGTGC TCGGTGGCTAGCCTGCTGCTGGACCCTTACTACCGGACTCTGAAAGGATTCATGGTATTAATTGAAAAGG ACTGGATTTCCTTCGGACATAAATTTAATCATAGATATGGCAACTTAGATGGTGACCCAAAAGAAATCTC TCCAGTAATCGACCAGTTCATTGAGTGTGTCTGGCAGCTAACGGAACAGTTCCCTTGCGCCTTTGAGTTC AACGAGAGGTTTCTGACTCACATTCAGCATCATGTTTATTCCTGCCAGTTTGGGAACTTCCTGTGTAATA GCCAGAAGGAGAGGCGAGAACTCAATGAGAATGGAAAAAGCAAAAAGGGTTCACGGTTCCTTAAGGAGGA CTCCTGAGGTTGAAGGCTAGCCTGTGTTGAAACCACAGCATACGATTCAAGAAAGAACCTACTCTTTGTG GTCCAACCTGTGGAAGAATCGAGCTGACTACCTCAATCCTCTGTTTAGAGCTGACCACAGTCAGACCCAG GGGAGCCTTCATCTGCCTACAGCTCCATGCAACTTCACCTATAATAATAATATAAACTTACTCTTCCTAA GAGAAGTTAACTACCTTAGAAAAAATATCAACCTGAAATTATTACTTTTAGTAGTTGCTTTTTCAAGGCT GTAACTTGCTTTTGGTTATTAATATGTTGGGCTGTGATACTGAAGACAAGACTTGGGATTGTGGGCTTTC AGACTGGGATATGTGCTATCCTTCCTTCCTTCCTCCAGGTTTTGGAATGGGATGTATAACCGCTTTGAAA AGGGGTTGCAGCCTCGACAGTCTGTTACAGATTACCTCATGGCTGTGAAGGAGGAGTCCCAGCAGCTGGA GGAAGAACTGGAGTCCCTAGAAGAAAGGCTGGAAAAAATTCAGAAAGTCCACTTACATGGTACCAAAGTG AAGAGTAAGCAAAGTGAGCCCAGCAAACACTCGGGGTTCTCTACCTCAGACCACAGCACAGCCAACACCC CTCAGGATTACAGTGGGAACAGCAAGTCCTTCCCATCCAGGAGCCCGTCCCAGGGTGATGAAGATTCTGC TCTGATTCTAACCCAAGACAATTTAAAAAGCTCAGACCCCGACCTCTCAGTGAATAGTGATCAAGAGTCT GGGGTGGAGGATTTGAGCTGTCGATCTCCCAGTGGTGGTGAGCATGCACCCAGCGAAGACAGTGGCAAGG ACCGGGACTCGGACGAAGCTGTGTTTCTCACTGCCTAAGTTCCCCTTTGGAGTTCCAAAGTAAAAGACAC AGAAACACTTTCAAATTAAGGGGAAAGTGCAATTCGTAGTAAAAATGTTGAGAAATGGCAAGTTCTATCC AGAACCATAAAATTACAGCTGTGAAAATGGGGAAAAAAAAGCTCTTTATCAATAACCAGTTGTTTTATCA AATCATACTGAAGACTGATCATCATTCCTGAGGCTCATGCTAGACTATGAAAATTATATCACTACAAGGA GAGAGAGTATCTTCACCCAAGGTTGTCCAGACAGTCTTTCCTGGCATAGCATGTGTCTGCTTAAAAGCCC TCCTGGTTTCTCCCATACCTGATAATGTTGGGACTTTGATTTCACTTCCAGTTGCAGGTTGCATTCAGGA ACTTTGACATTTCCTTCTGCCTTTACCAATATTTAAAAGAAATAAATATGGAGAGTACCCTTTTCTGTTA TAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5368816 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5368816 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11041 same embryo
	EMAGE:11039 same embryo
	EMAGE:11038 same embryo
	EMAGE:11042 same embryo
	EurExpress:euxassay_003206 same experiment
	MGI:4826498 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source