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EMAGE:11118

Kcnj14 potassium inwardly-rectifying channel, subfamily J, member 14 ( MGI:2384820)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11118 EMAGE:11118 EMAGE:11118 EMAGE:11118 EMAGE:11118
"Pseudo-wholemount" of euxassay_001130. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001130_01 euxassay_001130_02 euxassay_001130_03 euxassay_001130_04
EMAGE:11118 EMAGE:11118 EMAGE:11118 EMAGE:11118 EMAGE:11118
euxassay_001130_05 euxassay_001130_06 euxassay_001130_07 euxassay_001130_08 euxassay_001130_09
EMAGE:11118 EMAGE:11118 EMAGE:11118 EMAGE:11118 EMAGE:11118
euxassay_001130_10 euxassay_001130_11 euxassay_001130_12 euxassay_001130_13 euxassay_001130_14
EMAGE:11118 EMAGE:11118 EMAGE:11118 EMAGE:11118 EMAGE:11118
euxassay_001130_15 euxassay_001130_16 euxassay_001130_17 euxassay_001130_18 euxassay_001130_19
EMAGE:11118 EMAGE:11118 EMAGE:11118 EMAGE:11118 EMAGE:11118
euxassay_001130_20 euxassay_001130_21 euxassay_001130_22 euxassay_001130_23 euxassay_001130_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11118Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11118_wholemount_strong.wlz
11118_wholemount_moderate.wlz
11118_wholemount_weak.wlz
11118_wholemount_possible.wlz
11118_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11118_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
limb
weak weak
regionalweak expression: see section 01 02 03 07 08 09 10 23 24
adrenal gland
weak weak
regionalweak expression: see section 09 10 11 12 18 23 24
pancreas
weak weak
regionalweak expression: see section 11 12
hypothalamus mantle layer
weak weak
regionalweak expression: see section 09 10 11 12 14 15 16 17 18
diencephalon lateral wall mantle layer
weak weak
regionalweak expression: see section 08 09 10 11 12 14 15 16 17 18 19
telencephalon mantle layer
weak weak
regionalweak expression: see section 06 07 08 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
olfactory cortex marginal layer
moderate moderate
homogeneousmoderate expression: see section 11 12 13 14 16 17 18 19 20
hindbrain
weak weak
homogeneousweak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
midbrain mantle layer
weak weak
regionalweak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19
facial vii ganglion
weak weak
regionalweak expression: see section 04 21 22
glossopharyngeal ix ganglion
weak weak
regionalweak expression: see section 06 07 18
trigeminal v ganglion
weak weak
regionalweak expression: see section 03 04 05 06 07 08 18 19 20 21 22 23
vagus x ganglion
weak weak
regionalweak expression: see section 08 09 16 17
ventral grey horn
moderate moderate
regionalmoderate expression: see section 17 weak expression: see section 08 09 10 11 12 13 14 15 16 18
cervico-thoracic ganglion
weak weak
regionalweak expression: see section 10 16
cervical ganglion
weak weak
regionalweak expression: see section 09 16 17
thoracic ganglion
weak weak
regionalweak expression: see section 12 13 14 15
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 15 16 17 weak expression: see section 18 19
neural retina
weak weak
regionalweak expression: see section 02 03 04
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 12 16
lower jaw molar
moderate moderate
regionalmoderate expression: see section 07 08 20
oral epithelium
moderate moderate
regionalmoderate expression: see section 08 09 weak expression: see section 10 18 19 20
upper lip
moderate moderate
regionalmoderate expression: see section 06 07 08 22
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 13 17
upper jaw molar
moderate moderate
regionalmoderate expression: see section 07 10 21
axial skeleton
weak weak
regionalweak expression: see section 15 16 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4237
Entity Detected:Kcnj14, potassium inwardly-rectifying channel, subfamily J, member 14 ( MGI:2384820)
Sequence:sense strand is shown

>T4237
GTGCCCCGGCTCAGGCTCAGGCCCCAGGGGCCGAGGACAGACCTGAACACATTAGCACCCACAGAGGCCC
CTCTCCAGCCTGAGCAGAGGAACCCCCAGTGCAGGGAATGGGGGTGGGCGGCCCCCCCCAGGTTTGGGGC
GCCTGCCCCCCGCTAGGACAGATCTAGAGGGGGTCTCCCCACCCAATGGGCCTGGCTAGGGCCCTGCGCC
GCCTGAGCGGCGCGCTGGAGCCAGGAAACAGTCGAGCTGGGGATGAGGAAGAGGCGGGGGCCGGACTGTG
CCGCAATGGGTGGGCTCCTGGACCGGTAGCTGGGAGCCGGCGCCGCGGGCGCTTCGTCAAAAAAGATGGG
CACTGCAATGTGCGCTTCGTGAATCTGGGCGGCCAGGGCGCGCGCTACCTAAGTGACCTGTTCACCACAT
GTGTGGACGTGCGCTGGCGCTGGATGTGCCTGCTCTTCTCCTGTTCCTTCCTCGCCTCCTGGTTGCTCTT
CGGCCTGACCTTCTGGCTCATCGCCTCGCTGCACGGCGACCTAGCTGCCCCACCGCCACCGGCTCCCTGT
TTCTCGCAAGTGGCCAGCTTCCTGGCTGCCTTCCTCTTTGCACTGGAGACACAGACGTCCATAGGCTATG
GTGTGCGCAGCGTCACCGAGGAGTGTCCAGCTGCTGTGGCCGCTGTGGTGCTGCAGTGCATCGCAGGCTG
TGTGCTCGACGCCTTCGTCGTGGGTGCAGTTATGGCCAAGATGGCCAAGCCCAAGAAACGCAACGAGACA
CTAGTCTTCAGTGAGAACGCAGTGGTGGCTCTGCGAGACCACCGTCTCTGCCTCATGTGGCGGGTGGGCA
ACCTGCGACGCAGTCACCTGGTGGAAGCTCACGTGCGGGCCCAGCTGCTGCAGCCCCGTGTGACCCCCGA
GGGTGAGTACATCCCACTGGACCACCAGGATGTAGATGTTGGTTTTGATGGAGGCACTGATCGCATCTTC
CTCGTCTCCCCCATTACCATTGTGCATGAGATTGACTCTGCCAGTCCACTGTATGAGCTGGGGCGGGCCG
AGCTGGCCCGGGCTGACTTTGAGCTGGTGGTCATTCTTGAAGGTATGGTTGAGGCCACAGCCATGACTAC
ACAGTGCCGCTCATCTTACCTCCCAGGTGAGCTGCTCTGGGGACATCGCTTCGAGCCGGTCCTCTTCCAG
CGTGGCTCCCAGTATGAGGTTGACTATCGCCACTTCCATCGAACATATGAGGTCCCAGGGACACCAGTCT
GCAGTGCCAAGGAGCTGGATGAACGGGCAGAGCAGGCTTCCCACAGCCCTAAGTCAAGTTTCCCTGGCTC
CCTCACTGCATTTTGTTATGAGAATGAACTTGCTCTGAGCTGCTGCCAGGAGGAAGATGAGGAGGAGGAC
ACTAAGGAGGGGACTTCAGCAGAGACCCCAGAGAGGGCTGCCAGCCCCCAAGCTCTTACACCAACCCTGG
CTCTGACCCTGCCTCCATGACACAGCTGAGGCCCACTTCCCCATGTGACACCCCTTCCCAAAGATGACAG
CTTCCTTCCAGCCTCTGATGGGAGTGGGACAGGGGTTTCTCAGGCCCAGTGGCCTGCTGAGGTGACCATT
AACCACGAGGATGTCTACCACAGATAGACCACACCTTTATTTCTCTGCTCCATTCTCACTCCCGAGACCC
CTTTATGAGCTGGATTCCTGAGGCTTACCAGAGCCGAAGGATCAGATTTCTGGACCATACAAGAGACAAG
GCGTGGAGGCTGGGTTTTGGTTGAACATCAGTGAGATACACAGCGTCATGGAGGAAAATGTCAATTTACA
CAGATCAAGAAACAAGGATTCAGGATCATTATAATTGACCAAAGACAGAACAGTGACTGGACCGTGTGTG
GAGAAAAGGCCCAGTGGCTCCAGAAAGCCCAAGAGTTGAGATTCTCTTGAGTCTAAGTTGACACTGGGGA
TTCTAAAACAGGTTGGCTAGAGGAGAGTGGAGCTGCTCCAGAAACTACAAAGCTATGTTTCTAGAATACA
CAAAACTTTGGTAGGTTTCTGAGTGTGGAAGGACTAAGCTGACACACGAAGGTTCCAGATCCCGGAGGAC
TCTGGAGGACTGGCTGTGTAGGGTCCGGCTAGAGGGGGTAGGTAGGTCTGGCTTCTCCATGATCCACAGG
GTGAATTTTGTTTTTGTTTGAAGTGCCATTCTTAGAGCATCCAAAGGAATCAGGTTCTGTGGTTCAGGGC
TGATCAGAGTCCGGGTGGGATCCTAGCTGTCTGTGATTTGCTGGCTCCGCTCTGCCTAGCAGGGCTGGGG
ACCTCCATGACCTTGGCTGGCTTGCTATGGTTCTGTAATGCCAAAAGGAATCAAAACTCCAGAGACCAGC
GCTGTCCTGTCCCAAAGCCTAGAAGTCCTAGTCACCTTCAGATGGACAGCCTGACAGCTCTAGGAGGTAG
ACACTGCTCTGGGTGGAGCTTGCAAGCAAGAGCCTGGACGATCACAGAGCTAACGAATGGAAAAGGGAAG
GAAGAGGCTGAGGCCGGCAAGCCTAAAATATCCTGGATTACTATACCTAGTGATGTGTGTCTCTTTCTCA
CAGATATTATACAGATGTGTATTATATATGATATAAATACAGATCTCTAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4503027 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4503027 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11121 same embryo
 EMAGE:11119 same embryo
 EMAGE:11120 same embryo
 EurExpress:euxassay_001130 same experiment
 MGI:4825718 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS