EMAGE mouse gene expression spatial database

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EMAGE:11121

Syt13 synaptotagmin XIII ( MGI:1933945)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001015. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001015_01	euxassay_001015_02	euxassay_001015_03	euxassay_001015_04

euxassay_001015_05	euxassay_001015_06	euxassay_001015_07	euxassay_001015_08	euxassay_001015_09

euxassay_001015_10	euxassay_001015_11	euxassay_001015_12	euxassay_001015_13	euxassay_001015_14

euxassay_001015_15	euxassay_001015_16	euxassay_001015_17	euxassay_001015_18	euxassay_001015_19

euxassay_001015_20	euxassay_001015_21	euxassay_001015_22	euxassay_001015_23	euxassay_001015_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11121_wholemount_strong.wlz

11121_wholemount_moderate.wlz

11121_wholemount_weak.wlz

11121_wholemount_possible.wlz

11121_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11121_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thalamus mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 13 15 16 17 18 19 weak expression: see section 14

telencephalon mantle layer

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16 19 20 21 22 23

telencephalon ventricular layer

moderate

regional

moderate expression: see section 04 05 06 22 23 24 weak expression: see section 03 07 08 09 10 21

midbrain marginal layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

facial vii ganglion

moderate

homogeneous

moderate expression: see section 04 05 20 21 22

glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 07 08 18 19

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23

vestibulocochlear viii ganglion

moderate

homogeneous

moderate expression: see section 06 07 08 18 19 20

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 16 17 18 19

neural retina

moderate

regional

moderate expression: see section 02 03 04

tongue

weak

regional

weak expression: see section 14 15

lower jaw incisor

weak

regional

weak expression: see section 11 12 13 14 15 16 17 18

upper jaw incisor

weak

regional

weak expression: see section 12 13 14 15 17 18 19

genital tubercle of male

moderate

regional

moderate expression: see section 14 15 16 17 18 19

chondrocranium

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4254
Entity Detected:	Syt13, synaptotagmin XIII ( MGI:1933945)
Sequence:	sense strand is shown >T4254 GAGCGGCTGGAGCCAGGCGGGAGCGCGAGGTGCAGGAGGCCGGCGGGAGGCGGGCGAGCCGCGGGAAGCA GGCACCATGGTGCTGTCGGTGCCTGTGATCGCGCTGGGCGCCACGCTGGGCACTGCCACCAGCATCCTTG CGCTGTGCGGGGTCACTTGCCTGTGCCGACACATGCACCCCAAGAAGGGGTTGCTGCCGCGGGACCGTGA GCCCGACCCGGAGAAGGCAAGGCCCGGTGTGCTTCAGGCGGCCCAACAGTTCAACATTAAAAAGTCCACG GAGCCTGTCCAGCCCCGACCTCTCCTTAAGTTCCCGGACATCTATGGTCCCCGGCCAGCCGTGACGGCCC CAGAAGTCATCAACTACGCAGACTACACCTTGGAGACCACAGAGGAGTCTGCTGCACCTGCCAGCCCCCA GGCCCAGAGTGACAGTCGCCTCAAGAGGCAGGTCACAGAGGAGCTGTCCATCCGTCCTCAGAACGGTGTA GTTGAGGATGTCTGTGTCATGGAGACCTGGAACCCAGAGAAGGCTGCCAGCTGGAACCAGGCCCCCAAAC TCCACTTCCGCCTGGACTATGACCAGAAGAAGGCTGAGTTATTTGTGACAAGCCTGGAAGCTGTCACCAG TGACCATGAAGGAGGCTGTGACTGTTACATCCAAGGCAGCGTGGCTGTCAAGACCGGCTCTGTGGAGGCA CAGACAGCTCTGAAGAAGCGGCAGCTGCACACAACCTGGGAGGAGGGCCTGGCCCTGCCCCTGGGTGAGG AAGAGCTCCCCACAGCTACCCTGACACTCACCCTGCGCACCTGTGACCGCTTCTCCCGCCATAGTGTGAT TGGGGAACTCCGCCTGGGCCTGGATGGAGCATCTGTACCCCTGGGGGCAGCACAGTGGGGTGAGCTGAAG ACCACAGCAAAGGAGCCATCTGCAGGGGCCGGGGAGGTCCTTCTGTCAATCAGCTACCTCCCAGCTGCCA ATCGCCTCCTGGTGGTGCTGATTAAAGCTAAGAACCTCCACTCCAACCAATCCAAGGAGCTGCTGGGCAA GGATGTGTCTGTCAAGGTGACCTTGAAACACCAGGCTCAGAAGCTGAAGAAGAAACAGACAAAACGGGCC AAGCACAAGATCAACCCCGTGTGGAACGAGATGATCATGTTTGAGCTACCGGATGACTTGCTGCGGGCAT CCAGTGTGGAGCTGGAGGTGCTGGGCCAGGGCGAGGAGGGGCCGAGCTGCGAGCTGGGCCACTGCAGCCT AGGCCTGCATGCCTCGGGCTCTGAGCGCAGCCACTGGGAGGAGATGCTAAAAAACCCACGCCGGCAGATC GCCATGTGGCACCAACTGCACCTGTAGTCGGCCACCTGCCACCGGCCACTGACCGTCTTTCTTGGACACA GTCATCTTCCAGGCTCAGCTGTCAGTCATCCCTCGTGCTCTGTCCACATGCCTATACCCAAAGACAGATG CAGACGTGTTGGCTGAGGCCAAGACCTCATTCCTCTCTTACTGTATTCCCAATACTAAAAAGTGAGCGGT GGCGCCAGGACAGTGAGTGGGACTGGCACTCAGAAGACTGTATCATGCTCATCTGTGTTATGGAAAAAGA TATGTGCCAGGTACCCTCAGTCGGCAGCCCCTGCTCACATTGAGCTGAAGCAGTGGTTCTTAACCTGTGT ATCACAACCCTCTATCTCCAAAAAATATTTACATTATGATTCATAACAGTAGCAAAATTACAGTTACAAA GTAGCAATGAGAACAATTCTGTGGTTGGAGATCACCAAAACACAAGAAACTTTCTTATAGGGTCACAGCA CTAGGAAGGTTGAGAATCACTGGCTGGCCAGCAGGTCTCTGGGCCACTGGGGGGTCATGCCACACTGATG TTCCGGTGGAATACACAAGCCTCTGTCCTTAGCTAGCACAGGGGTTCTGGAGCAGAGCAGGAGGGGCTGC GCTTGATAAGATCCCTTCTCCCCTATCCAGGCAGCCTTTCCTCTTGATCTCCGGGGGCCCAGCACCAGGA CAGTGTGAAGGCAGGAACTCTCTGCTTACTTTATTTCTTCTTTTTTATATTTCTGCTGTTCATATTCCAC TCTCGAGCTACATTGTGTGTTTTGACTGTTTTATTTAAGGGATTGGAGTGGGTTGTGTTTGTTTTTTTTT TATCACACGTAGAGTGCCCGTTCCCTGGCAGGCCTAGGTTGCCACTCCTCTGGAGTGTCTGCGGCATTCT GAGGACAGCTGTCATGTCACCCCCCCCCACCCCCAAGCTGACTCCATGTGACAGACCTGTTCTTCTCAGT ATGCCTGTACTTCAGGACGAAGTAGCTCTGAGCTTGGCTTGTTCCATCTGGCTATGTAAATGAGGAGGTA GATGAACTTTAAAGAGAAAACGTGGTCTCTTGATAACATGCCTCCCTCCAGTTTGGGGGACATGTCCTTT AACTTGGAAATAACAAAAAGCTAGGTTTGATTGCCCCTGTTGATATTTCCAATAGATGCCCCACTAGGAA TCAAATAAGTAGCTAGCTGCAGGGGTAGATACTTGTGAGCCGGAATTCGAGTTGTCATTACTTCCTTGCT CCACAGCGCCTCAGTGTGGCCACCTATAGCACAGCACCAATGTCATGGGAGCTTGCCAGTCCAGCTTCAG GAGGTAAGCAAGAGGCATCTTAGCGGGCATCAATTGTTACTGGTCAGTTGGTGGCACTTCCTGGCTCCTC CGTTGGCTGTGCTTCTTCTCCAAAACCTCCTCTGTAAATTGCTCAGAGATGTTAGACTGGGAACCAAAAA CTTTCAGTCACCTTTTACATCGGCTTAATCTGTTTCCAGCCATTCCTGTAAGTCACCTCCGAATCAGCTT GGGAAGGAACCTCTCTATGTCTTTGCCAGGGAGTGATTTCTGGAAAGAGGTGGAAATAAAGTGAGAGGCC ATTCCAGGCTGGGCTACTGCTGGGAGGTTAGGAGTTCACCCCAGTCCTCTGTGAATCCTCTCCCCAGGAC AACTAGGCATCTATTTGTTGAGTGTTTGTCCACCAAGACCGTGCATGTGTCTGTCCATTCTGATTAGAGA AGGCTCTGGGGGCGAGTGAGTAGCTTATCACCTTAACGGAAACATCAGATAAGAGGAGTCCGGGACTGCT GTTCAGGCGGGCAGCTCTCAGATGTAAAGGGGCCCCGGGGGAAGGGGCAGTGTCTGGCCTGAACTGAGGT GGGAATCTTTGGAGGAGAAAGTTGGTCCTGTGCAGTGTTTCTACCCTTGCTGTTTCCTGTTGTTAGATGC TGCGTGTTTGTGATGCCATTCCACATAAAAGCACAAAAAGTCGCATGAGACATCGTGGTCACATGTCTGG TTACACTTTGGAGCACAAAATCTTACAGTGGTAAATAAATCGTTTTCCAATCGAAAAAAAAAAAAAAAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4503633 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4503633 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11118 same embryo
	EMAGE:11119 same embryo
	EMAGE:11120 same embryo
	EurExpress:euxassay_001015 same experiment
	MGI:4828568 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source