EMAGE mouse gene expression spatial database

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EMAGE:11131

Kif2c kinesin family member 2C ( MGI:1921054)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003601. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003601_01	euxassay_003601_02	euxassay_003601_03	euxassay_003601_04

euxassay_003601_05	euxassay_003601_06	euxassay_003601_07	euxassay_003601_08	euxassay_003601_09

euxassay_003601_10	euxassay_003601_11	euxassay_003601_12	euxassay_003601_13	euxassay_003601_14

euxassay_003601_15	euxassay_003601_16	euxassay_003601_17	euxassay_003601_18	euxassay_003601_19

euxassay_003601_20	euxassay_003601_21	euxassay_003601_22	euxassay_003601_23	euxassay_003601_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11131_wholemount_strong.wlz

11131_wholemount_moderate.wlz

11131_wholemount_weak.wlz

11131_wholemount_possible.wlz

11131_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11131_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

moderate

regional

moderate expression: see section 03 04 05

hindlimb

moderate

regional

moderate expression: see section 06 07 08 09 21 22 23 24

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

thymus primordium

strong

regional

strong expression: see section 17 moderate expression: see section 13 14 15 16

submandibular gland primordium

strong

regional

strong expression: see section 08 09 10 19 20 21 moderate expression: see section 11 18

pancreas

moderate

regional

moderate expression: see section 10 11 12

thyroid gland

moderate

regional

moderate expression: see section 13 17

forebrain

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

strong

regional

strong expression: see section 04 05 06 07 08 20 21 22 moderate expression: see section 23 24

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13 14 15

telencephalon ventricular layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain

moderate

single cell

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

4th ventricle

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23

midbrain

moderate

single cell

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

spinal cord

moderate

single cell

moderate expression: see section 11 12 13 14 15 16 17 18 19

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 23

retina

moderate

regional

moderate expression: see section 01 02 03 04 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 13 15 16 17 18 19 20 21

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19

esophagus

moderate

regional

moderate expression: see section 15

tongue

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19

midgut

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

lower jaw incisor

strong

regional

strong expression: see section 12 13 17 moderate expression: see section 14 16 18

lower jaw molar

moderate

regional

moderate expression: see section 07 08 19 20 21

upper jaw incisor

strong

regional

strong expression: see section 12 13 17 moderate expression: see section 14 16 18

upper jaw molar

moderate

regional

moderate expression: see section 07 08 19 20 21

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

bladder

moderate

regional

moderate expression: see section 13 14 15 16 17

metanephros

moderate

regional

moderate expression: see section 09 10 11 12 13 19 20 21 22 23

genital tubercle of male

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19

testis

moderate

regional

moderate expression: see section 08 09 10 21 23

lung

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23 24

orbito-sphenoid

moderate

regional

moderate expression: see section 03 04 05 06 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6621
Entity Detected:	Kif2c, kinesin family member 2C ( MGI:1921054)
Sequence:	sense strand is shown >T6621 GCGGTTTAGGCGTTGTTAACACAGCGCAGTATTAGCAGAGTCGTGGTTTCCAAGCTTCTTTCATTTGTGT TGCCTGTTGTTGTTCCTGAGTCCATGGAGTCGCTTCACGCACGCCTGTTTCCTGGTCTCTCCATCAACAT TCAACGCAGCAATGGCTTAATTCACCCCGCCAATATAAGCACAGTGAACGTGGAGAAATCCTGTGTTTCG GTGGAATGGATCGAAGGAGGTACCACAAAAGGCAAAGAGATTGACATTGACGATGTGGCTGCAATAAACC CAGAGCTCCTACAGCTTCTTCCCTTACGACCGAAGGACAGTCTGCCACTGCAGGAAAACGTAACGGTCCC GAAGCAAAAACGCAAATCAGTCAACTCCAAAATTCCAGCTCTAAAAGAAGGCCTTCGAAGCCGCTCCACT CGCATGTCCACTGTCTCAGAAGTTCGAATCCCTGCTCAGGAGAATGAAATGGAGGTGGAGCTGCCTGTGC CTACAAACTCTCGCAAGCAGTTTGCCATTCCCAGCCACCCTCGGGCCTCCTGCTCCACAGTGACAGAATT GCCGTTGTTGATGGTCAGTGAGGAGGCAGAAGAGCAAGCCCACTCCACCCGCAGTACCTCATCTGCAAAC CCTGGGAACTCAGTTCGGAGGAAATCGTGTATCGTGAAGGAGATGGAGAAAATGAAGAACAAGCGTGAAG AGAAGAGGGCCCAGAACTCGGAGCTAAGAATAAAGCGAGCACAGGAATATGACAGCAGCTTTCCAAACTG GGAATTTGCCCGGATGATCAAAGAATTTCGGGTTACTATGGAATGTAGCCCGCTTACTGTGACAGACCCT ATCGAAGAGCACAGGATCTGTGTCTGTGTCAGGAAACGCCCACTGAATAAACAAGAACTGGCCAAGAAAG AAATCGATGTGATTTCTGTTCCCAGCAAGTGTCTCCTCCTGGTGCATGAACCCAAGTTAAAAGTGGACTT AACGAAGTACCTGGAGAACCAAGCTTTCTGCTTTGACTTTGCATTTGATGAGACAGCTTCCAATGAAGTT GTCTACAGGTTCACAGCAAGGCCACTGGTACAGACGATTTTTGAAGGGGGAAAAGCAACATGCTTTGCCT ATGGACAGACAGGCAGTGGGAAGACACATACAATGGGTGGAGATCTGTCTGGTAAAAGTCAGAACGCATC TAAAGGGATCTACGCCATGGCTTCCCGGGATGTCTTCCTTCTGAAGAACCAGCCTCGGTACCGGAACTTA AACCTGGAAGTTTATGTGACATTCTTCGAGATCTACAATGGGAAGGTGTTTGATCTGCTCAACAAAAAGG CCAAGTTACGTGTGCTGGAGGACAGCAGGCAGCAGGTGCAGGTTGTGGGACTGCAGGAGTACCTGGTCAC CTGTGCTGATGATGTCATCAAGATGATCAACATGGGCAGTGCCTGCAGGACTTCTGGACAAACATTTGCC AACTCCAATTCCTCCCGCTCCCACGCCTGCTTCCAGATTCTTCTTCGAACTAAAGGGAGATTACATGGCA AATTCTCTTTGGTGGATCTGGCAGGGAATGAACGAGGGGCCGACACATCTAGTGCTGACCGGCAGACTCG CATGGAGGGAGCAGAAATTAACAAGTCTCTCCTGGCTCTGAAGGAGTGCATCCGGGCGCTGGGACAGAAC AAGGCTCACACCCCGTTCCGTGAGAGCAAGCTGACCCAGGTGCTGAGGGACTCGTTCATTGGGGAGAACT CGAGGACCTGCATGATCGCCATGATCTCACCAGGCATAAGCTCCTGTGAATACACCTTAAACACGCTGAG ATACGCAGACAGAGTCAAGGAGCTAAGCCCCCACAGCGGACCCAGTGGAGAGCAGCCGGTTCAGATGGAA ACAGAAGTGATGGAAGCCAGCTCTAATGGGACGTCTCTGACTGGCAATGAAGAGGAGGAGCTGTCTTCCC AGATGTCCAGCTTTAATGAAGCCATGACTCAGATCAGGGAGCTGGAAGAGAGGGCCCTGGAAGAGCTCAG AGAGATCATACAGCAAGGACCAAACTGGCTTGAGCTCTCCGAGATGACAGATCAGCCAGACTATGACTTG GAGACCTTTGTGAACAAAGCAGAATCTGCCCTGACACAGCAGGCTAAGCAGGCGAAGCACTTCTCAGCCC TTCGAGAAGTCATCAAGGCCCTGCGCCTAGCCATGCAACTGGAAGAACAGGCTAGCAAACAAATAAACAG CAAGAAACGGCACCAATGATGACTTCAAATAAAGATCTGTTTGATATCAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3598157 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3598157 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11132 same embryo
	EMAGE:11129 same embryo
	EMAGE:11128 same embryo
	EMAGE:11133 same embryo
	EMAGE:11130 same embryo
	EurExpress:euxassay_003601 same experiment
	MGI:4825763 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source