EMAGE mouse gene expression spatial database

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EMAGE:11136

Fiz1 Flt3 interacting zinc finger protein 1 ( MGI:1344336)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003444. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003444_01	euxassay_003444_02	euxassay_003444_03	euxassay_003444_04

euxassay_003444_05	euxassay_003444_06	euxassay_003444_07	euxassay_003444_08	euxassay_003444_09

euxassay_003444_10	euxassay_003444_11	euxassay_003444_12	euxassay_003444_13	euxassay_003444_14

euxassay_003444_15	euxassay_003444_16	euxassay_003444_17	euxassay_003444_18	euxassay_003444_19

euxassay_003444_20	euxassay_003444_21	euxassay_003444_22	euxassay_003444_23	euxassay_003444_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11136_wholemount_strong.wlz

11136_wholemount_moderate.wlz

11136_wholemount_weak.wlz

11136_wholemount_possible.wlz

11136_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11136_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 11 12 13 14 15

submandibular gland primordium

moderate

regional

moderate expression: see section 06

vibrissa

moderate

regional

moderate expression: see section 06 07 08 09 21 22 23

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 11 12 16 17 18

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 09 10 11 12 13 15 16 17 18 19 20 21 22 23 weak expression: see section 08

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22

midbrain ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16

dorsal root ganglion

weak

regional

weak expression: see section 08 09 10 12 13 16 17 18

pharyngo-tympanic tube

weak

regional

weak expression: see section 01 02 03 21 22 23 24

neural retina

moderate

regional

moderate expression: see section 01 02 03 weak expression: see section 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 13 15 16 17 18 19 20

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 13 14 16 17

esophagus

moderate

regional

moderate expression: see section 13 weak expression: see section 12 14

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 13 16 17 18

lower jaw molar

moderate

regional

moderate expression: see section 07 20

upper jaw incisor

moderate

regional

moderate expression: see section 12 13 16 17 18

upper jaw molar

moderate

regional

moderate expression: see section 07 20

renal cortex

moderate

regional

moderate expression: see section 07 08 09 10 11 17 18 19 20

maturing glomerular tuft

moderate

regional

moderate expression: see section 07 08 09 10 17 18 19 20

testis

weak

regional

weak expression: see section 05 06 07 08 20 21 22

trachea

weak

regional

weak expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5921
Entity Detected:	Fiz1, Flt3 interacting zinc finger protein 1 ( MGI:1344336)
Sequence:	sense strand is shown >T5921 GAAAATGGAGAGGTTTATATAAAGTGGGGCCTAGCAAAGAGGCGTGAATCTCATGAAATGGGGGAAAGGG CCTTGGTTGAAAAATTGAAGAAACTTCGTAAGATGAGGGGAAAACTGGGGTCCAGTAAGACAACAGAGGC CCAGAAGAAAGCCCCAAGCCACCTCCGGACCTTCCGTCATGGAAGACTCCTCGCTGCCTGTGGTTCCTGC CCCCATCGCTGCTCCAGGACCAGCACCATCTGCCACTGCGCCTCGGGTCCCCTTTCACTGTAGCGAATGT GGCAAGAGTTTCCGTTACCGATCAGACCTGAGGCGCCACTTTGCTCGACACACGGCTCTCAAGCCCCACG CATGTCCTCGATGCGGTAAGGGCTTCAAGCATAGCTTCAACTTGGCTAACCACCTGCGCTCACACACTGG TGAACGACCCTACCGATGCTCTGCCTGCCCTAAGGGATTCCGAGACTCCACTGGCCTGCTGCACCACCAG GTGGTCCACACTGGTGAAAAGCCCTACTGCTGTTTGGTCTGTGAGCTCCGCTTCTCCTCTCGCTCCAGCC TGGGCCGCCATCTCAAGCGGCAGCACCGGGGCACACTTCCCTCTCCCCTGCAGCCTAGCCCCGGCCTGCC TCCCCTGAGCTCCCCCTGCTCAGTATGTTGCAACGTGGGTCCCTGTTCGGTTTGTGGAGGTGGAGGGTCC AGTGGAGGAGAGGGCCTGGAGGGAGCAGGAGCAACCAGCTGGGGACTGGCAGAGGCTGCAGCAGCCGCGG CAGCCTCGCTGCCCCCATTTGCATGCGGTGCCTGTGCCAGGCGTTTTGACCATGGCCGGGAGCTTGCAGC CCACTGGGCCGCACACACCGATGTGAAGCCATTCAAGTGCCCTCGCTGTGAGCGCGACTTCAACGCACCT GCGCTGCTGGAACGCCACAAGCTCACTCATGACCTGCAGGGGAGCAATGCACCTCCAACGCAGGTTTGGG CCTCGGGTGGGGGTCCGGAGGTGGCAGGAGAGGGAGATGCCTCAGAGGTGGGGGCCGCCCCCCAGACTTG GGACGCCGGGCTGCTCCTGAGCCCTACCGGGGCAGGCGTGCCCAAGCTGGAGGCATTGCTCCCGGGGGAC GAGGGCTCTGGGAATGACCAGGCTCCGGCTGCAGCAGCCGAAGCATCTTCGGAAGACACACTGTACCAGT GCGACTGCGGGACCTTCTTCGCATCTGCCCCGGCTTTGGCCAGCCATTTAGAGGCTCACTCAGGCCCAGC CACTTATGGCTGTGGCCACTGCGGGGCGCTGTACGCAGCGCTGGCTGCCCTGGAGGAGCATCGGCGTGCA AGCCATGGTGAGGGTAGCGGGGAGGCAGCCCCCGATGGTGAGGGCAATCAGGCGGCTGACGGGCCGGGAC CCGGCTCCTCCAGCCGTAGCAAGAAGATCTTTGGCTGTTCCGAATGTGAGAAGCTGTTCCGTTCACCTCG AGACCTGGAAAGGCATGTGTTGGTCCATACCGGGGAAAAGCCGTTCCCGTGCCTGGAGTGTGGCAAGTTC TTCCGCCACGAGTGCTACCTCAAGCGTCACCGGCTGCTGCATGGCACAGAGCGGCCCTTCCCCTGCCACA TCTGTGGCAAGGGCTTCATCACGCTTAGCAACCTCTCCAGGCATCTGAAGCTACACAGGGGCATGGACTG AATAACCAGGACACCCCTGCCCACTGAGTCCAGAGCCAGACCGTAAGACAAGCCAGGCCCCTGCCTGGAA AAATGGTGCCACCCAGAACCCACAGTGAAACATCCTTCAGCAAAGGGGGTCCAAGAACATAATTGTTGGG GAATTTCTCTCTGAAACCTACAGAGACTATTCACTTTGAGACCCCTGGAATGTGGCATGTTCAAATCAAC TGGCCTCCAAGACTGGGTACATAGTAATGAAAAACGGGTCTCCACTAACATCTCTCTGAAGATAACATGT AGGCACCCCCATGATTTTAACCACCCTGTGAGCCAGCATTCCAGATCCTTCATCTAGAGAATGGGGGTGG GGCAATATCTTAGACTCCTCCAGGAACCCCTCAAATGACACCTGCCAGCCACTGGTGACCCCAGAAAATG AGTATAACCGAAATCTGCCTTCCGCCAATTTTGCTTCCTGTACTGAAAGAAGATCAGGGTAGATGCCCCC CACCTTCAGTTAACCCACTCTCAGATTAGGTCTCCCTTACTGCAGAATGGTCTGACCCTACTGATCTTCC CCCATCCTGAACACTAGATTAGACTGGCCCTTTAGGTTGGACTAACCCAGATGCACATACTCCTATCCAC TTGGAATGGGCTGGTGTCTGGAATGGTTGTGGCCTAATCCCACTCTCCTTAGAGTAATAGGGCAGACATT CCCTTATGCTGTAGAGCACAGTCCTTTGACCACGATTTCCCAATCCTGTGACCTGGTGGAGGTGGTAGAC ATTGAGGCTTTCCCTACCCCTTGACTTGTAGTACTTTGGTCTTTCCATCACTACAACTTGACTCTTTGTT CCAACGTCCCAAGGGGATATGGGAACTTGGCCAGGGGTTTCTCTCCTGTGAGCCTCAACCTGACCCCTGA CCCCTCTCCCCAATGTCTCTGGGACTCCAATAAACCTCATTCAGTCAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3482191 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3482191 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11139 same embryo
	EMAGE:11138 same embryo
	EMAGE:11137 same embryo
	EMAGE:11135 same embryo
	EMAGE:11134 same embryo
	EurExpress:euxassay_003444 same experiment
	MGI:4824867 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source