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EMAGE:11191

Emb embigin ( MGI:95321)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11191 EMAGE:11191 EMAGE:11191 EMAGE:11191 EMAGE:11191
"Pseudo-wholemount" of euxassay_001159. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001159_01 euxassay_001159_02 euxassay_001159_03 euxassay_001159_04
EMAGE:11191 EMAGE:11191 EMAGE:11191 EMAGE:11191 EMAGE:11191
euxassay_001159_05 euxassay_001159_06 euxassay_001159_07 euxassay_001159_08 euxassay_001159_09
EMAGE:11191 EMAGE:11191 EMAGE:11191 EMAGE:11191 EMAGE:11191
euxassay_001159_10 euxassay_001159_11 euxassay_001159_12 euxassay_001159_13 euxassay_001159_14
EMAGE:11191 EMAGE:11191 EMAGE:11191 EMAGE:11191 EMAGE:11191
euxassay_001159_15 euxassay_001159_16 euxassay_001159_17 euxassay_001159_18 euxassay_001159_19
EMAGE:11191 EMAGE:11191 EMAGE:11191 EMAGE:11191
euxassay_001159_20 euxassay_001159_21 euxassay_001159_22 euxassay_001159_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11191Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11191_wholemount_strong.wlz
11191_wholemount_moderate.wlz
11191_wholemount_weak.wlz
11191_wholemount_possible.wlz
11191_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11191_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
adrenal gland
strong strong
homogeneousstrong expression: see section 09 10 11 17 18 19
thymus primordium
moderate moderate
homogeneousmoderate expression: see section 11 12 13 14 15
submandibular gland primordium
strong strong
regionalstrong expression: see section 07 08 09 10 16 17 18 19
pancreas
moderate moderate
regionalmoderate expression: see section 10 11 12 13
pituitary gland
moderate moderate
homogeneousmoderate expression: see section 11 12 13 14 15 16
choroid fissure
strong strong
regionalstrong expression: see section 15
lateral ventricle choroid plexus
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 16 17 18 19 20
4th ventricle
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22
medulla oblongata basal plate
moderate moderate
regionalmoderate expression: see section 08 09 10 11 16 17 18
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 05 06 07 08 20 21
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 08 18
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 16 17 18 19 20 21 22
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 09 10 16 17
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 20
ventral grey horn
weak weak
regionalweak expression: see section 10 11 12 13 14 15
cervico-thoracic ganglion
weak weak
regionalweak expression: see section 10
cervical ganglion
weak weak
regionalweak expression: see section 10
thoracic ganglion
weak weak
regionalweak expression: see section 11 12 13 14 15
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19
pharyngo-tympanic tube
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 20 21 22 23
neural retina
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 22 23
external naris epithelium
strong strong
regionalstrong expression: see section 11 12 13 14 16 17 18
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 16 17 18 19 moderate expression: see section 15
esophagus
weak weak
regionalweak expression: see section 13 14
stomach
moderate moderate
regionalmoderate expression: see section 09 10 11 12 weak expression: see section 03 04 05 06 07 08
lower jaw incisor
strong strong
regionalstrong expression: see section 12 moderate expression: see section 11 weak expression: see section 13 15
lower jaw molar
moderate moderate
regionalmoderate expression: see section 07 20 weak expression: see section 19
upper jaw incisor
strong strong
regionalstrong expression: see section 12 16 moderate expression: see section 13 weak expression: see section 15
upper jaw molar
moderate moderate
regionalmoderate expression: see section 07 08 weak expression: see section 19
liver lobe
moderate moderate
spottedmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
kidney calyx
strong strong
regionalstrong expression: see section 08 09 11 12 17 18 20 21
kidney pelvis
strong strong
regionalstrong expression: see section 10 19
genital tubercle of male
weak weak
regionalweak expression: see section 13 14 15 16 17 18 19
larynx
moderate moderate
regionalmoderate expression: see section 13 14 15
trachea
strong strong
regionalstrong expression: see section 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4211
Entity Detected:Emb, embigin ( MGI:95321)
Sequence:sense strand is shown

>T4211
CCCAGTTACCAGCAAGTGTACACAGGGACCAACGGAGACGGATCGCAGCGAGCTGAGGGTGCCTTAGCGA
CCTCCTGGACAGACCCGCCCGCGCCATGCGCTCGCACACTGGCCTGAGGGCGCTTGTGGCGCCTGGTTAC
CCGCTCCTCCTTCTATGTCTTCTGGCGGCGACGCGCCCTGATCCGGCTGAGGGCGATCCCACAGATCCAA
CTTTTACAAGTCTACCTGTTCGAGAAGAAATGATGGCGAAATACTCAAACCTTTCCTTAAAGAGCTGTAA
TATCTCAGTGACGGAAAAGTCCAATGTATCAGTAGAAGAGAACGTAATTTTGGAAAAGCCTTCTCATGTG
GAACTCAAATGCGTGTACACAGCAACTAAGGATTTGAACTTGATGAATGTGACTTGGAAGAAAGATGATG
AGCCCCTTGAGACTACGGGTGACTTCAATACAACTAAAATGGGCAACACCTTAACCAGTCAGTACAGGTT
CATCGTTTTTAATAGCAAACAATTGGGAAAATATTCTTGTGTCTTTGGAGAAAAGGAACTAAGAGGGACC
TTTAACATCCACGTACCCAAAGCTCATGGGAAAAAAAAGTCGTTGATCGCTTACGTGGGGGATTCTACTG
TGCTGAAGTGTGTATGTCAAGATTGTCTTCCTTTAAATTGGACTTGGTACATGGGTAATGAAACCGCACA
GGTTCCCATTGACGCTCACTCGAATGAAAAGTATATCATCAATGGTTCCCATGCCAATGAAACAAGGCTC
AAGATTAAGCATCTTTTGGAGGAAGATGGAGGATCCTACTGGTGTCGTGCCACCTTCCAGTTAGGGGAGA
GTGAGGAGCAGAATGAGCTGGTTGTGCTGAGCTTCCTGGTGCCCCTCAAGCCATTTCTGGCCATACTTGC
CGAAGTCATCCTCTTGGTGGCCATCATTCTGCTTTGTGAAGTGTACACACACAAGAAAAAGAATGACCCA
GATGCTGGGAAAGAATTTGAACAAATTGAACAGCTGAAATCAGATGATAGCAATGGCATAGAAAACAATG
TCCCCCGGTACAGAAAAACTGACTCTGCAGATCAGTGAATACAGAAAGAGCATCTCGCCCTGGGAAGAAG
TACAGCGTTGGAGTTTGTACTTCAGCTTCCTTATTTTAGATTTTTAAAAGACAAAGATGCCCTCAACGTG
CACAGAAGTAGCTTTATGAACAATATAGTCCATCTCCTACCTAAAGATGTACTTTCATTTTGTAATTGCT
TTCCATTAAAGCAAGGTCAATTGTTTCAGCTGTGTTCTGTGAGCTGTAACCTAGATAATAAACTCCCCTC
TGTGGGTACAGGGTGGACACCAGCAAAATCACTTAATAATCCACAAATAAATATCGCCGAAGATCTGTTT
ATTACCAAAGAATTTATTAAAGAGAAAGCGTTTGCCAGTTGTCTTAAGTGTTTGCCTCCACCAGACATCT
TACCTATAGAAGTATTTATCACAGTTTTCATGTGAAGTGTATCCAGTGTTGAGTGTGATTTCTCTAGGAA
ATGGAAAGTATGCTTTCAATATCGGTGGCTACACTGCAGTCTAGACACTAAGATAAACTTGTGCTCTATA
AATAATGGTGCAAAGTAAGGGAGTTCAAATGCTTGTGCCATGGCCACTACATTACCAGAGCTTCTCATCT
TCATTGCACTCATTTCTGCTCTTTCTATCCCAAGCCGAAGAGCTTCCCAGCCTTAAGTAGAACATTTTCC
TTGTTCTTTGTCATTGGGGTTTGGACTTCAATAGTCTTCAGAGGCCAGATCCACGTTGTCACAGTACAAC
CCCGCTCGCTACCTGCATGCCTTTGTGGCTCATGGTTTCTGGGTGCCTTTATGTGCCATCTCTTTCCTGG
TACCATACCTGGCTAAAATGCATTCTTAGTGTTTTCCAATAATAATTCCTTTGTCTAGTATACAGGAGGA
CTAGTGCACTATCTTTATGTTGCTGCGAATGCCTTAGACTTAACTTCAAAATTGTATCTGAGATACTTTA
TGAACTTAGAACCAAATCACGTCAGAATTTTAATGGACATGGAAACTTGTCAATGGTTTTAAATGAAATA
AAATGTGCCTTGGATGGTTGGATTAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4500922 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4500922 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11187 same embryo
 EMAGE:11188 same embryo
 EMAGE:11189 same embryo
 EMAGE:11190 same embryo
 EurExpress:euxassay_001159 same experiment
 MGI:4824536 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS