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EMAGE:11195

Ctse cathepsin E ( MGI:107361)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11195 EMAGE:11195 EMAGE:11195 EMAGE:11195 EMAGE:11195
"Pseudo-wholemount" of euxassay_003472. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003472_01 euxassay_003472_02 euxassay_003472_03 euxassay_003472_04
EMAGE:11195 EMAGE:11195 EMAGE:11195 EMAGE:11195 EMAGE:11195
euxassay_003472_05 euxassay_003472_06 euxassay_003472_07 euxassay_003472_08 euxassay_003472_09
EMAGE:11195 EMAGE:11195 EMAGE:11195 EMAGE:11195 EMAGE:11195
euxassay_003472_10 euxassay_003472_11 euxassay_003472_12 euxassay_003472_13 euxassay_003472_14
EMAGE:11195 EMAGE:11195 EMAGE:11195 EMAGE:11195 EMAGE:11195
euxassay_003472_15 euxassay_003472_16 euxassay_003472_17 euxassay_003472_18 euxassay_003472_19
EMAGE:11195 EMAGE:11195 EMAGE:11195 EMAGE:11195 EMAGE:11195
euxassay_003472_20 euxassay_003472_21 euxassay_003472_22 euxassay_003472_23 euxassay_003472_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11195Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11195_wholemount_strong.wlz
11195_wholemount_moderate.wlz
11195_wholemount_weak.wlz
11195_wholemount_possible.wlz
11195_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11195_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
forelimb
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05
hindlimb
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 22 23 24
thymus primordium
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15
submandibular gland primordium
strong strong
regionalstrong expression: see section 07 08 09 17 18 19 20
thyroid gland
moderate moderate
regionalmoderate expression: see section 10 11 14 15
vibrissa
strong strong
regionalstrong expression: see section 08 09 10 22 23 24 moderate expression: see section 07 11
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 05 06 07 18
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 07 08 09
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 06 07 18
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 06 07 08 09 11 12 13 14 15 16 17 18 19
not examined not examined
regionalnot examined expression: see section 01 02 03 04 05 23 24
neural retina
moderate moderate
regionalmoderate expression: see section 02 03 weak expression: see section 04 05 24
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 17 18 19 20 21 22
nasal cavity respiratory epithelium
moderate moderate
regionalmoderate expression: see section 14 15 16 18 19 20
lower jaw incisor
strong strong
regionalstrong expression: see section 17 moderate expression: see section 12 13 14 18 19
lower jaw molar
moderate moderate
regionalmoderate expression: see section 07 08 09 10 20
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 13 14 15 18 19
upper jaw molar
moderate moderate
regionalmoderate expression: see section 07 08 09 10 20
liver
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
metanephros
moderate moderate
regionalmoderate expression: see section 08 09 10 11 18 19 20 21 22
larynx
moderate moderate
regionalmoderate expression: see section 11 12 13 14
lung
strong strong
regionalstrong expression: see section 03 04 05 06 07 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
not examined not examined
regionalnot examined expression: see section 06 07 08 09 11 12 13 14 15 16 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6678
Entity Detected:Ctse, cathepsin E ( MGI:107361)
Sequence:sense strand is shown

>T6678
CAACTTCATTTGTTCCTCAGAACTGCAGATGCCCTGTGTGACTCCAGAAGCGGTTAGAAAGATCAGGCGT
GGTTATCTCCATTTGCAGCTGAGGTGGCCAAGAGATAAGAGTTGCTTAAAGTCGGAGTTAGCAAACAACA
AAACGGGGTTTCCACACAGAGCTCAGCCTCGGAGCCCAACCCATCCACTTACTACAAGAAAACGAAATTC
TTGACATTTTGGGTTATATCCGGTAACTTCAAAGACTGGGCAGGGCAGGTCTGGGCACCAGGAAGAGCCA
GTGCCCCTGCCCTGTGATCAAGAGGAAAGAAGGGTGGAAGGGAGATCGGAGCAGAGTGAGAGAGAAGCTA
CCCCTTGGACCACAATGAAGCCCCTCCTTGTGCTGCTGCTGCTGCTGCTCCTGGATCTGGCTCAGGCCCA
AGGTGCTCTGCACAGAGTGCCCCTCAGAAGACATCAGTCCCTTCGGAAGAAACTACGGGCCCAAGGACAG
CTCTCAGAATTCTGGAGGTCTCATAACTTGGACATGACCCGACTCAGCGAGTCCTGTAATGTGTATTCGA
GTGTCAATGAACCCCTCATCAACTACCTGGATATGGAATACTTTGGCACCATCTCCATCGGCACCCCGCC
GCAGAACTTCACTGTCATCTTTGACACCGGTTCATCCAACCTCTGGGTCCCTTCTGTGTACTGCACCAGC
CCAGCATGCAAGGCACACCCAGTATTCCATCCATCGCAGTCCGACACATACACGGAGGTAGGGAATCATT
TCTCCATCCAGTATGGTACCGGGAGCCTGACAGGAATCATTGGAGCTGATCAAGTCTCTGTGGAAGGGTT
GACTGTGGATGGCCAGCAGTTTGGAGAAAGTGTCAAGGAGCCAGGCCAGACCTTTGTGAATGCAGAGTTT
GATGGGATTCTGGGTCTGGGATACCCCTCATTGGCTGCTGGAGGAGTGACCCCAGTGTTTGACAACATGA
TGGCCCAGAACCTTGTGGCTCTGCCTATGTTTTCTGTCTACTTGAGCAGTGACCCTCAAGGTGGCTCAGG
CAGTGAGCTGACTTTCGGAGGCTATGACCCCTCTCATTTCTCTGGGAGCCTCAACTGGATTCCAGTCACC
AAGCAAGCCTATTGGCAGATTGCCTTGGATGGAATCCAGGTGGGAGACACTGTGATGTTCTGCTCCGAAG
GCTGTCAGGCCATAGTGGACACAGGGACCTCTCTCATCACTGGCCCCCCCGACAAGATCAAACATCTTCA
AGAGGCCATTGGGGCCACACCCATTGATGGAGAATATGCAGTGGATTGTGCCACTCTCGACACGATGCCA
AACGTTACCTTCCTCATCAACGAGGTTTCATATACCCTCAACCCAACTGACTACATCCTGCCGGACTTGG
TGGATGGAATGCAGTTCTGCGGCAGTGGCTTTCAAGGACTTGACATTCCACCTCCAGCTGGGCCCCTCTG
GATCCTGGGGGATGTCTTCATCCGACAGTTCTACTCAGTCTTTGACCGTGGAAATAACCAAGTGGGATTG
GCCCCCGCAGTTCCCTAAAGAGGGATGTATGCCTACATATGGATGCCTGATACCCATTTAACCTGTTAGA
TACCTTTGTAACTATCAAAGCCGTCATTTCCCATGGGGTGTAGCCACCCCAGAGTATTCAGACCAATCAA
AGCATAAGAGTGCACCCCACTCACTGCAAACACACACACACACACACCACCTCTACCATCACCACGATGG
AAGAAGTTCTGTCTATAGTCTTCACTGCTTATTGTTGACTTTCTATTATGGAAATCTCTAAACATGTACA
CAGTAGACGTGATGGCAAGATAAATACCCACACACCTCTGCCTCAGGTCACAACCCATCCATGTGTGGAC
CAGACTCTCTATCTTCCATCCCTCTGGTTCCACGCCTCTAGATTTGGAAGCAGATTCTAAGCACCAGGTC
ATTTTATCTAATGTCTAACATCCTTACAAATCAGAATTTAAATGCCTCACCCTCTCATAAATGTGGATCT
GTTTTTTACAGTTGGTTTATTTGTATCAGGATTAAAACCAGATCCATAACTGGACAAAAAAACCCATAAA
CTGATTTGATTTTAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3601519 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3601519 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11194 same embryo
 EMAGE:11197 same embryo
 EMAGE:11193 same embryo
 EMAGE:11196 same embryo
 EMAGE:11192 same embryo
 EurExpress:euxassay_003472 same experiment
 MGI:4824112 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS