EMAGE mouse gene expression spatial database

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EMAGE:11199

Adi1 acireductone dioxygenase 1 ( MGI:2144929)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003618. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003618_01	euxassay_003618_02	euxassay_003618_03	euxassay_003618_04

euxassay_003618_05	euxassay_003618_06	euxassay_003618_07	euxassay_003618_08	euxassay_003618_09

euxassay_003618_10	euxassay_003618_11	euxassay_003618_12	euxassay_003618_13	euxassay_003618_14

euxassay_003618_15	euxassay_003618_16	euxassay_003618_17	euxassay_003618_18	euxassay_003618_19

euxassay_003618_20	euxassay_003618_21	euxassay_003618_22	euxassay_003618_23	euxassay_003618_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11199_wholemount_strong.wlz

11199_wholemount_moderate.wlz

11199_wholemount_weak.wlz

11199_wholemount_possible.wlz

11199_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11199_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 13 14 15 16 17

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 10 11 18 19 20 21 22

pancreas

weak

regional

weak expression: see section 11 12 13 14 15 16 17 18 19 20

thyroid gland

moderate

regional

moderate expression: see section 12 13 17

vibrissa

moderate

regional

moderate expression: see section 22 23 24 weak expression: see section 07 08 09 10

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 04 05 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14 16 17 18 19 20 21

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 14 17 18

esophagus

moderate

regional

moderate expression: see section 13 14

stomach

weak

regional

weak expression: see section 09 10 11 12

hindgut

weak

regional

weak expression: see section 15 16 17 18 19

rectum

weak

regional

weak expression: see section 16 17

midgut

weak

regional

weak expression: see section 10 11 12 13 14 15 16 17 18 19 20 21

lower jaw incisor

weak

regional

weak expression: see section 13 14 17 18 19

upper jaw incisor

weak

regional

weak expression: see section 13 14

liver left lobe

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14

liver right lobe

weak

regional

weak expression: see section 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6617
Entity Detected:	Adi1, acireductone dioxygenase 1 ( MGI:2144929)
Sequence:	sense strand is shown >T6617 AAGCAACGCCCCTTCCAGCCAGTTAGTGGAAGCCGCCGCCGCCACCATGGTGCAGGCCTGGTATATGGAC GAGTCCACCGCCGACCCGCGGAAGCCCCACCGCGCACAGCCCGACCGCCCCGTGAGCCTGGAGCAGCTGC GCACGCTCGGAGTGCTCTATTGGAAGCTAGATGCTGACAAGTATGAGAACGATCCAGAACTGGAAAAGAT CCGGAAAATGAGAAACTACTCCTGGATGGACATCATCACCATATGCAAAGATACACTTCCCAATTACGAG GAGAAGATCAAGATGTTCTTTGAGGAACATCTGCATCTGGATGAGGAGATCCGCTACATCCTGGAGGGCA GTGGGTACTTCGATGTCAGGGACAAGGAGGACAAGTGGATCCGGATTTCCATGGAGAAGGGGGACATGAT TACTCTTCCTGCTGGCATCTATCACCGCTTCACACTGGACGAGAAGAATTACGTGAAGGCCATGCGGCTG TTTGTTGGAGAACCTGTGTGGACACCATACAACCGGCCAGCTGACCATTTTGATGCCCGTGTACAGTACA TGAGTTTTTTGGAAGGAACAGCATAGCAGTGCTCCTCGAAGAGAAAACTGCACTGTGTGAATCTCCTGCT GTGGTAACCGAATGGAAAGTTGCTCACTTTTCTGCTTTTCTATATGAACTTGAGGCTAGACTAGCTCTCT TTGCTAAGATTGTGAGATCAGTGTCTTTAAATGAAAGCATCTCTAAAAGTGATTTTTACATGGAAGCCAC AAAAATGTGAAAAAGTGACCTTAATTTTCCCTAACTGTCAAGACTTAGAGGTATAGGAGCCCTGGATTGG TATGTGCATTCATGCATGGCCAATCTTCATCTCCCAGATCTTTAGGTGTCTGTTGGTGTGAAGCTATGCC TCCTGCAAGAGGGCAGTTATAACCAGCACAACTAACCAGATGACGTTTTCCTCCTTTGCTGATTGTTGAT TGGGGAAGTGGGGTTGTTGTTCTTTCTTAAAACTAAGTTACCTTAATTGTATGCTCACAGCTTAGCTGTA TGTACATTCATGTGTACCTACAGTATTTTACTTGGTTCATTAAAACCTCCCATTTAGATTCCTTATGTGA TTTTTATATGTACATAGTTTCTATATCAGGGTGAAAGATCCTGACAGAATGTTAAAGGTTACAGAAGCCC TGAAATTGGCAAAATAGATTTCATTGTTAACAGAACTAGCTTCACTGATTTAGAAAAATAGAGGTGCACA ATGCTCTGGCCGCTCCTTGAACCCGTGTGTCTGCCAATGTTTTGACCGTTCCTTGAACCCATGTGTGTGC CCACTGAAGAAGCCCATTGCCAGGTGTTTGTGATATTGGTTGCTGGGGAAGAGTCGGAAAAATCTCCCCA GCAACCACAGCCGAGCCAATCCGGAGTTGCTACACCTGCACCAGACTCTTTCTTGTACCCCTCCCATACC CATTTCTTGAGAAATCATTTTAGAATAGATATTGTTTAGATTGGGAAATCCCCTACTCCTTCCTTCTCCT TTCCCCCCTGAGGGCCTATAAAAACTGGGACCCCTTTCTCCTCGGGGTCAACTCCTCTACCCCTGCGTGG GATATGAGTCATTCCCAGAGCTCTGGCTTTCCCCAATAAAGCCTCGTGTGGTTTGCATCAAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:3597652 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3597652 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11198 same embryo
	EurExpress:euxassay_003618 same experiment
	MGI:4823008 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source