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EMAGE:11201

Col8a1 collagen, type VIII, alpha 1 ( MGI:88463)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11201 EMAGE:11201 EMAGE:11201 EMAGE:11201 EMAGE:11201
"Pseudo-wholemount" of euxassay_006534. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006534_01 euxassay_006534_02 euxassay_006534_03 euxassay_006534_04
EMAGE:11201 EMAGE:11201 EMAGE:11201 EMAGE:11201 EMAGE:11201
euxassay_006534_05 euxassay_006534_06 euxassay_006534_07 euxassay_006534_08 euxassay_006534_09
EMAGE:11201 EMAGE:11201 EMAGE:11201 EMAGE:11201 EMAGE:11201
euxassay_006534_10 euxassay_006534_11 euxassay_006534_12 euxassay_006534_13 euxassay_006534_14
EMAGE:11201 EMAGE:11201 EMAGE:11201 EMAGE:11201 EMAGE:11201
euxassay_006534_15 euxassay_006534_16 euxassay_006534_17 euxassay_006534_18 euxassay_006534_19
EMAGE:11201 EMAGE:11201
euxassay_006534_20 euxassay_006534_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11201Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11201_wholemount_strong.wlz
11201_wholemount_moderate.wlz
11201_wholemount_weak.wlz
11201_wholemount_possible.wlz
11201_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11201_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
medulla oblongata part of 4th ventricle choroid plexus
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 13
rib
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 16 17 18 19 20 21
forearm mesenchyme
strong strong
regionalstrong expression: see section 01 02
upper arm mesenchyme
strong strong
regionalstrong expression: see section 01 02 03 04 05 18 19 20 21
forelimb digit 1 mesenchyme
strong strong
regionalstrong expression: see section 02 03 04 05 06
forelimb digit 2 mesenchyme
strong strong
regionalstrong expression: see section 02 03 04 05 06
forelimb digit 3 mesenchyme
strong strong
regionalstrong expression: see section 02 03 04 05 06
forelimb digit 4 mesenchyme
strong strong
regionalstrong expression: see section 04
hindlimb digit 1 mesenchyme
strong strong
regionalstrong expression: see section 20
hindlimb digit 2 mesenchyme
strong strong
regionalstrong expression: see section 06 07 09 10 20 21
hindlimb digit 3 mesenchyme
strong strong
regionalstrong expression: see section 06 07 08 09 10 20 21
hindlimb digit 4 mesenchyme
strong strong
regionalstrong expression: see section 06 07 08 09 10 20 21
hindlimb digit 5 mesenchyme
strong strong
regionalstrong expression: see section 07 08 09 20 21
lower leg mesenchyme
strong strong
regionalstrong expression: see section 02 03 04 05
upper leg mesenchyme
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 12 18 19 20 21
vibrissa dermal component
strong strong
regionalstrong expression: see section 04 05 06 07 20
diencephalon lateral wall mantle layer
strong strong
single cellstrong expression: see section 09 10 11 12 13 15 16 17
diencephalon roof plate
strong strong
regionalstrong expression: see section 14
choroid invagination
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 16 17
telencephalon mantle layer
moderate moderate
single cellmoderate expression: see section 01 02 03 04 05 06 07 18 19 20 21
medulla oblongata alar plate mantle layer
strong strong
single cellstrong expression: see section 07 10 14
medulla oblongata basal plate mantle layer
strong strong
single cellstrong expression: see section 13
metencephalon floor plate
strong strong
regionalstrong expression: see section 12 13
rest of cerebellum mantle layer
strong strong
single cellstrong expression: see section 16
pons mantle layer
strong strong
single cellstrong expression: see section 06 07 09 10 13 16 17
metencephalon part of 4th ventricle choroid plexus
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 13
midbrain mantle layer
strong strong
single cellstrong expression: see section 09 10 11 12 13 15 16 17
spinal cord floor plate
strong strong
regionalstrong expression: see section 13
spinal cord mantle layer
strong strong
single cellstrong expression: see section 09 10 11 13 14 15
otic capsule
strong strong
regionalstrong expression: see section 05 06 07 08 09 16 17
naris
strong strong
regionalstrong expression: see section 12 13 14 15 16 17
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 16 17 18
nasal septum
strong strong
regionalstrong expression: see section 15
viscerocranium
strong strong
regionalExpression in the turbinate bone.
aorta
strong strong
regionalstrong expression: see section 11 12 13 14
umbilical artery embryonic part
strong strong
regionalstrong expression: see section 15
heart valve
strong strong
regionalstrong expression: see section 10 11 12 14
tongue
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16
mandible
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18
maxilla
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19
bladder
strong strong
regionalstrong expression: see section 14 15
genital tubercle of male
strong strong
regionalstrong expression: see section 15 16 17
arytenoid cartilage
strong strong
regionalstrong expression: see section 12 13
cricoid cartilage
strong strong
regionalstrong expression: see section 12 13
thyroid cartilage
strong strong
regionalstrong expression: see section 11 12 13 14
trachea cartilaginous ring
strong strong
regionalstrong expression: see section 13 14
axial skeleton
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18
basioccipital bone
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
basisphenoid bone
strong strong
regionalstrong expression: see section 08 09 11 12 13 14 15 16
vault of skull
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 08 09 10 19 20 21
sternum
strong strong
regionalstrong expression: see section 12
pelvic girdle skeleton
strong strong
regionalstrong expression: see section 10 11 12
axial skeleton tail region
strong strong
regionalstrong expression: see section 14 15 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6767
Entity Detected:Col8a1, collagen, type VIII, alpha 1 ( MGI:88463)
Sequence:sense strand is shown

>T6767
GAGCCCTTCCACGGTCCTCTCCGTGGGAGCCCGAGTCCCTGTCTCCCAGATCTGACGTGCTCAAGAAGCT
GTTCTGTAAATCAAGTAAAACCTGTGAAGAGAGTGCACCCAGCCCCAGTGGTATTACAAGAAGAGTCAAG
CAAGGACTTTGGTCCTCGATTCTACTGGCCAGCCAAGCCTAAATGTGATGGCACTGCCACCCAGGCCTCT
ACAGCTGCTGGGAATACTGTTCATCATTTCCCTGAACTCTGTCAGACTCATTCAGGCCGGTGCCTACTAT
GGAATCAAGCCTCTGCCACCTCAAATCCCTCCTCAGATACCACCACAAATTCCACAGTACCAGCCCTTGG
GCCAGCAAGTCCCTCACATGCCTTTGGGCAAAGATGGCCTTTCCATGGGCAAGGAGATGCCTCACATGCA
GTATGGCAAAGAGTACCCACACCTACCCCAATATATGAAGGAAATCCCACCTGTGCCAAGAATGGGCAAG
GAAGTGGTGCCCAAAAAAGGCAAAGGAGAAGTACCGTTAGCCAGTTTGCGCGGAGAACAAGGTCCCCGTG
GAGAACCTGGACCAAGAGGACCACCTGGGCCGCCAGGTTTACCAGGTCATGGAATGCCTGGAATCAAAGG
AAAACCAGGGCCCCAGGGATATCCAGGAATTGGAAAGCCCGGTATGCCTGGAATGCCTGGGAAGCCAGGA
GCCATGGGAATGCCAGGGGCAAAAGGCGAAATTGGACCCAAAGGGGAAATCGGACCTATGGGAATCCCAG
GGCCACAAGGGCCTCCAGGACCTCATGGACTTCCTGGCATCGGGAAACCGGGTGGGCCAGGGTTACCAGG
GCAACCAGGAGCAAAAGGTGAGAGGGGGCCCAAAGGACCACCAGGACCTCCCGGCCTTCAGGGTCCCAAA
GGAGAGAAGGGCTTTGGGATGCCTGGCTTGCCAGGTCTGAAGGGTCCTCCAGGTATGCATGGCCCTCCTG
GACCAGTTGGACTACCGGGAGTAGGAAAACCAGGAGTGACAGGCTTTCCTGGACCACAGGGTCCCCTGGG
GAAGCCAGGGCCTCCAGGGGAACCTGGACCACAAGGCCTTATTGGTGTGCCAGGAGTTCAAGGACCTCCT
GGGATGCCTGGAGTTGGAAAGCCAGGTCAGGATGGGATCCCGGGCCAACCAGGATTTCCGGGTGGTAAAG
GGGAACAAGGATTGCCAGGGTTGCCTGGACCCCCAGGCCTCCCAGGGGTCGGAAAGCCAGGTTTCCCAGG
ACCCAAAGGGGACCGGGGTATTGGGGGTGTTCCTGGGGTTCTTGGGCCGAGAGGGGAAAAAGGACCCATT
GGTGCTCCTGGAATGGGGGGTCCACCTGGAGAGCCAGGCCTACCCGGTATCCCGGGTCCCATGGGCCCTC
CAGGTGCTATTGGTTTCCCTGGACCCAAAGGAGAAGGTGGAGTTGTAGGACCACAGGGTCCACCAGGCCC
CAAGGGAGAGCCTGGCCTCCAAGGCTTCCCTGGGAAGCCAGGTTTTCTTGGTGAAGTAGGTCCCCCTGGC
ATGAGGGGTTTGCCTGGTCCTATAGGACCCAAGGGAGAAGGTGGTCACAAAGGGTTGCCAGGGCTTCCCG
GTGTTCCAGGGCTGCTTGGACCCAAAGGAGAACCTGGCATACCTGGGGATCAGGGTCTACAGGGTCCCCC
AGGGATTCCAGGGATCGTAGGACCTAGTGGCCCTATTGGACCCCCTGGGATTCCGGGCCCCAAAGGAGAA
CCAGGCCTCCCAGGGCCCCCTGGGTTCCCTGGTGTAGGGAAGCCAGGAGTAGCAGGACTTCATGGGCCCC
CGGGGAAACCTGGTGCCCTTGGGCCTCAAGGCCAGCCTGGCCTTCCTGGACCCCCAGGTCCTCCAGGACC
CCCAGGCCCTCCAGCTGTGATGCCTACACCATCACCCCAGGGAGAGTATCTGCCAGATATGGGACTAGGA
ATTGATGGGGTGAAACCTCCGCATGCCTATGCGGGCAAAAAGGGCAAACACGGAGGGCCAGCCTATGAGA
TGCCTGCGTTTACTGCCGAGCTGACTGTACCTTTCCCACCGGTGGGGGCCCCAGTGAAGTTTGACAAGCT
GCTCTACAACGGCAGACAGAACTACAATCCGCAGACAGGCATCTTCACCTGTGAAGTCCCGGGTGTCTAC
TACTTTGCTTATCATGTTCACTGCAAGGGAGGCAACGTATGGGTTGCTCTCTTCAAGAACAACGAGCCCA
TGATGTACACATACGACGAGTACAAGAAGGGCTTTCTGGACCAAGCATCTGGAAGCGCAGTACTGCTTCT
CCGTCCCGGAGACCAGGTGTTTCTCCAAATGCCTTCAGAACAGGCTGCTGGACTCTATGCCGGGCAATAT
GTCCACTCCTCCTTTTCAGGATATTTATTGTATCCCATGTAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3674027 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3674027 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11202 same embryo
 EMAGE:11200 same embryo
 EMAGE:11203 same embryo
 EurExpress:euxassay_006534 same experiment
 MGI:4823994 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS