EMAGE mouse gene expression spatial database

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EMAGE:11203

Prkar1b protein kinase, cAMP dependent regulatory, type I beta ( MGI:97759)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006547. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006547_01	euxassay_006547_02	euxassay_006547_03	euxassay_006547_04

euxassay_006547_05	euxassay_006547_06	euxassay_006547_07	euxassay_006547_08	euxassay_006547_09

euxassay_006547_10	euxassay_006547_11	euxassay_006547_12	euxassay_006547_13	euxassay_006547_14

euxassay_006547_15	euxassay_006547_16	euxassay_006547_17	euxassay_006547_18	euxassay_006547_19

euxassay_006547_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11203_wholemount_strong.wlz

11203_wholemount_moderate.wlz

11203_wholemount_weak.wlz

11203_wholemount_possible.wlz

11203_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11203_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium mesenchyme

strong

regional

strong expression: see section 13 moderate expression: see section 04 05 06 07 12 14 15

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 13 14 15 16 17 18 19 20

cerebral cortex marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 19 20

telencephalon mantle layer

strong

regional

strong expression: see section 09 10 moderate expression: see section 01 02 03 04 05 06 07 08 11 12 13 14 15 16 17 18 19 20

olfactory cortex mantle layer

strong

regional

strong expression: see section 09 11 12 13

olfactory cortex marginal layer

strong

regional

strong expression: see section 07 08 09 12 13 14 15

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 04 moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15

rest of cerebellum mantle layer

strong

regional

strong expression: see section 03 04 14 15 16 17 moderate expression: see section 05 06 07 08 09 10 11 12 13

pons mantle layer

strong

regional

strong expression: see section 04 moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15

midbrain mantle layer

strong

regional

strong expression: see section 09 moderate expression: see section 04 05 06 07 08 10 11 12 13 14 15 16

facial vii ganglion

strong

homogeneous

strong expression: see section 04 05 15 16 17

glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 04 05 13 14

trigeminal v ganglion

strong

homogeneous

strong expression: see section 03 04 05 06 13 14 15 16 17 18

vagus x ganglion

strong

homogeneous

strong expression: see section 05 13

vestibulocochlear viii ganglion

strong

homogeneous

strong expression: see section 04 05 13 14 15

trigeminal v nerve

strong

regional

strong expression: see section 06 07 13 14

spinal cord mantle layer

strong

regional

strong expression: see section 08 09 12 13 moderate expression: see section 06 07 10 11 14

cervico-thoracic ganglion

strong

regional

strong expression: see section 06 07 08 11 12

cervical ganglion

strong

regional

strong expression: see section 06 12 13

thoracic ganglion

strong

regional

strong expression: see section 08 09 10 11

dorsal root ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14

neural retina

strong

regional

strong expression: see section 01 02 03 19 20

naris

strong

regional

strong expression: see section 11

nasal cavity olfactory epithelium

strong

spotted

strong expression: see section 09

tongue

strong

spotted

strong expression: see section 10

tongue extrinsic muscle

moderate

regional

moderate expression: see section 06 07 11 12

stomach

strong

spotted

strong expression: see section 02 03 04 05 06 07 08 09 10

midgut

strong

spotted

strong expression: see section 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6768
Entity Detected:	Prkar1b, protein kinase, cAMP dependent regulatory, type I beta ( MGI:97759)
Sequence:	sense strand is shown >T6768 CGCGTCCTCGCGCAGCTGCCTCCGGCCTCGGGCACCGCGCATCTACAGGAGCATAGAAGACATTCTGACC ATGGCCTCCCCATCATGCTTCCACTCGGAGGACGAGGACTCTCTGAAAGGATGCGAGATGTACGTGCAGA AACATGGCATCCAGCAGGTGCTCAAAGAATGCATTGTGCACCTCTGTGTCGCCAAGCCGGACCGGCCACT GCGATTCCTCCGGGAGCACTTTGAGAAGTTGGAGAAGGAGGAAAACAGGCAGATCCTGGCTCGGCAGAAG TCAAACTCCCAGTGTGATTCCCACGATGAGGAGATCTCCCCAACACCCCCAAACCCCGTGGTCAAGGCGC GTCGCCGGCGGGGTGGTGTGAGTGCTGAAGTCTACACTGAAGAAGATGCTGTCTCCTACGTGAGGAAGGT CATTCCCAAGGACTATAAGACCATGACCGCGCTGGCCAAGGCCATTTCCAAGAACGTGCTCTTTTCTCAC CTGGACGACAACGAGAGAAGTGACATATTTGACGCCATGTTTCCTGTCACTCACATCGGTGGGGAAACAG TCATACAGCAAGGGAATGAAGGAGATAATTTCTATGTGATTGACCAAGGAGAAGTAGATGTATATGTGAA CGGGGAATGGGTGACCAACATCAGTGAGGGGGGAAGCTTCGGGGAGCTGGCTCTCATCTACGGCACCCCC AGAGCGGCTACCGTGAAGGCCAAGACGGACCTCAAGCTCTGGGGTATCGACCGTGACAGCTACAGGCGCA TCCTCATGGGAAGCACGCTGAGGAAACGCAAGATGTATGAGGAGTTCCTCAGCAAAGTCTCCATCCTAGA ATCCCTGGAGAAGTGGGAACGCCTGACTGTAGCTGATGCCCTGGAGCCTGTGCAGTTTGAAGATGGAGAG AAAATTGTTGTGCAGGGGGAGCCTGGAGATGACTTCTACATCATCACAGAGGGCACTGCTTCAGTCCTCC AGCGACGATCCCCCAATGAGGAGTACGTGGAAGTGGGGCGCCTTGGACCCTCTGACTACTTTGGGGAGAT TGCCCTGCTGCTGAATCGGCCCCGTGCAGCCACTGTGGTGGCCCGGGGTCCCCTCAAGTGTGTGAAGTTA GACCGGCCTCGTTTTGAGCGTGTCCTGGGCCCCTGCCCTGAGATCCTGAAGAGGAACATCCAGCGTTACA ACAGCTTCATCTCCCTAACTGTCTGAGCTTGTGTTGCGCCTGCACCCCTGGGGGCCTCCCCATGTGGCCC TGTGACCCATTTCCTATGCCAGAGGCCTGGGAGCTGCTGGTTAGTAGCTGAGTGCGGCTGGTGCCCGGCC CGGAGCCTCCCCTTTCTCTGGACTCACTTTTGGAATAAATAACCATCTTGTGCTTCTTCAAAAACAAAGG GCAAGAGGCAACTGCGTCCCCAGACCAGGGCAGTGACAGCTGGCTCCCCCTGCAGCCTTATCTTGGGGCC ACCTTATCCCCTCTCCAAGTCTTCTGGGAATTTTTGTCTGATCCCCATACTTGAACTGGCCCTGTGTCCT GCCAGGTCTCAAGAGTGGGGCTGTGGCCACCCTTGTAGCTTTCCCCCAGAGCTGGGAGTTAATCAGAACA CTGTCCCTGGTCCTTGTAAGATTCTCTGGTCTAGGCCCAAGTGTAAAACAGATTGTGACAAATGGAGATG TCCTCAAATGAGCACTCAAGCTGAGAGAAGAAACAGAGGTTGAAAGGCATTCTTGGTCCCTGCGCACTGT CCCCAACTCTCAGAAGGACCCCTGAAGGACCCAAATTGATCCTAGCCTCCTATCCAGAGACTAAGCTATG GCCCCAGAAAGTCAGGAGGTGGTGGCTGAGCCCTCTCTACTCTCACAGCGCTTGGGAAGTCCATTTCCGG GTAGGCCAGGCACCCTGACCTCATGTCAGGACAGTTGGCCAAGCCCGTGACCCCTTACTGGCTGGACGCC CAGATTGGGCAGGAAGTGTGGCTGACGGCGGGGCTTTCCAAACGTGGTGAGTCTGGGCTGAGTCCCCATC TCCTGGAGACCAGGAGCCATGACTCTGTCAACCTGCCTATTGGAGACCCCAAGGAACAGCAAGCAACTCC AACTTGCTAGTTAAAGTTGTAGTTTTGAGCCTTCCCAGACTCTCTCTGTAAAGCCCGAATCCCTGTCCCT TGACTTCCAAACACCTCTCACCCGAGTCTCTCTGTTCCCTTACAAGAGGGGATAATGTACTGAAACCAGG CTTGACCCCACATCCCTAAACCATCCACCGTGGAGTGTGATATGAGCCAGCCAGACCCAAGGGCCACATG AGTGTCCACAACTGCTTAGTGTCCCCTGGGTGACCGGCATGTCATGAGACAGTGTGATCAACCTTTTCTT GCCCCGCTGTGCACGTCTAGGTGACCCTTCCAGCTGACCATCAGCTCCCCGCTGTGTGGCCCTGGCCATG TGTGTTGTGCCTGCACTGTGGACTATCTGTGGATTCTGTGGCCCTGCTTCTCCATCCCCTATTTATGTCA CCAAGCGCTGCATGAACTATTAAATGTGCAATGAAGAACCCTTTCCCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3674751 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3674751 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11201 same embryo
	EMAGE:11202 same embryo
	EMAGE:11200 same embryo
	EurExpress:euxassay_006547 same experiment
	MGI:4827419 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source