EMAGE mouse gene expression spatial database

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EMAGE:11216

Afap1l2 actin filament associated protein 1-like 2 ( MGI:2147658)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003397. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003397_01	euxassay_003397_02	euxassay_003397_03	euxassay_003397_04

euxassay_003397_05	euxassay_003397_06	euxassay_003397_07	euxassay_003397_08	euxassay_003397_09

euxassay_003397_10	euxassay_003397_11	euxassay_003397_12	euxassay_003397_13	euxassay_003397_14

euxassay_003397_15	euxassay_003397_16	euxassay_003397_17	euxassay_003397_18	euxassay_003397_19

euxassay_003397_20	euxassay_003397_21	euxassay_003397_22	euxassay_003397_23	euxassay_003397_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11216_wholemount_strong.wlz

11216_wholemount_moderate.wlz

11216_wholemount_weak.wlz

11216_wholemount_possible.wlz

11216_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11216_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 11 12 14

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13 14

olfactory cortex marginal layer

strong

regional

strong expression: see section 09 10 11 moderate expression: see section 12 14 15 16 17 18

telencephalon ventricular layer

moderate

regional

moderate expression: see section 03 04 05 06 07 19 20 21

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 11 12 15 16

pons ventricular layer

moderate

regional

moderate expression: see section 05 06 07 17 19 20 21 weak expression: see section 08 09 10

midbrain ventricular layer

moderate

regional

moderate expression: see section 11 12 14 15 16 17 weak expression: see section 08 09 10

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 18 19 20 21 22 23

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 09 18 19 20

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 16 17 18 19

esophagus

moderate

regional

moderate expression: see section 12 13 14

stomach

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10

hindgut

moderate

regional

moderate expression: see section 14

rectum

moderate

regional

moderate expression: see section 14 15

midgut

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20

midgut loop

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15

renal medullary interstitium

moderate

regional

moderate expression: see section 07 08 09 10 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6827
Entity Detected:	Afap1l2, actin filament associated protein 1-like 2 ( MGI:2147658)
Sequence:	sense strand is shown >T6827 CCGTGCCCGGGATGGAGCGCTACAAAGCACAGGGCTGTTGCTGTCTGGTGGTGCAGAGAAGAATCCTGCA GGTCTCTGCTTCCCTGGAGCAGCTGCTGACAGAGCTGGACGACTTCCTTAAGGTCCTCGACCAGGAGAAC CTGAGCAGTGCAGCCGTGCTGAAGAAGAGCGGCCTGTCAGAGCTCCTGCGGCTTTACACCAAGAGCAGCA GTTCTGATGAGGAGTATATTTACATGAACAAAGTGTCAGTCAACGGGGAACAAAACTCAGCATCTCCAGA CAAAGTTCCCGAGGAGCAGGGTCCACTGACCAATGGGGAGCCCAGCCAGCATTCCTCAGCCCCTCAGAAG AGCCTTCCAGACCTCCCTCCGCCCAAGATGATTCCAGAGAGGAAACAGCCCACCGTTCCAAAGATCGAGT CCCCTGAGGGCTACTATGAAGAGGCTGAGCCATTTGACAGATCCATCAATGAGGATGGAGAGGCTGTGAG CAGCTCCTACGAGTCCTACGATGAGGACGAGAACAGCAAAGGCAAGGCTGCCCCCTACCAGTGGCCCTCG CCCGAGGCCAGCATTGAGCTGATGCGTGATGCCCGCATCTGTGCCTTCCTGTGGCGCAAGAAGTGGCTAG GCCAGTGGGCCAAGCAGCTCTGTGTGATCAGGGACACCAGGCTCTTGTGCTACAAATCGTCCAAAGACCA CAGTCCTCAGCTGGACGTGAACCTGCGGGGCAGCAGCGTCGTGCACAAGGAGAAGCAAGTGCGGAAGAAA GGACACAAGCTCAAGATCACACCGATGAACGCTGACGTTATCGTGCTGGGCCTGCAGAGCAAGGACCAAG CTGAGCAGTGGCTTCGGGTCATCCAGGAGGTGAGTGGCCTGCCTTCTGAAGGAGCCTCAGAGGGAAACCA GTACACACCAGATGCCCAGCGCCTCAACTGTCAGAAACCAGACATAGCTGAGAAGTACCTGTCAGCAGCG GAGTACGGGATCACCATCAACGGCCACCCTGAGATCCCAGAGACCAAAGATGTCAAGAAGAAATGCTCTG CTGGCCTCAAGCTGAGCAACCTTATGAACCTGGGCAGGAAGAAATCTACCTCGCTGGAGCCCCCGGAGAG ATCCCTTGAGACATCCAGCTACCTGAATGTGCTGGTGAACAGTCAATGGAAGTCACGCTGGTGCTTCGTT AGAGACAGCCACCTGCACTTCTACCAGGACCGGAACCGGAGCAAGGTGGCCCAGCAGCCCCTCAGCCTGG TGGGCTGTGACGTGCTTCCAGATCCTAGCCCCGACCACCTGTATTCCTTCCGTATTCTCCACAACGGCGA GGAGCTAGCCAAGCTTGAGGCCAAGTCTTCAGAGGAGATGGGCCACTGGCTAGGCCTCCTGCTCTCAGAG TCGGGCTCCAAGACTGACCCGGAAGAGCTCACCTACGACTATGTGGATGCTGAAAGGGTTTCCTGTATTG TGAGTGCGGCCAAAACCTCTCTCTTACTGATGCAGAGAAAGTTCTCAGAGCCCAACACATACATCGATGG CCTGCCCAGCCGGGATTGCCAGGACGATCTGTATGATGATGTGGAGGTATCAGAGCTGATAGCCGTGGTG GAGCCTGCCGAGGAAGCCGCCCCTGCTGTCGATGCTAACAGTGGTAGTGAGCCAGACAGAGTCTACCTGG ATCTCACACCGGTCAAGTCCTTCCTGCACAGTAGCAGTGAGGCTCAGGCGCAGGCCTCTCTCCCAGCAGT GCCACATCAGGACGATGTAGCTGAGACCCTCACAGTAGACCCAAAGCCAGGCACCACCCCAGAGGAGCCC CACACAGAGTCTCCAGGAGACCCAGAGGTGCAGCAGAGGCAACCAGAGGTTCAAGAGTCTTCAGAGCCTA TCGAGCCCACCCCGCGAATCACCATGGTTAAGCTACAGGCCGAGCAGCAGAGAATCTCCTTCCCAGCCAA CTGCCCAGACACCATGGCTTCTGCCCCCATCGCTGCCAGCCCACCTGTGAAGGAGAAGCTGAGAGTGACC AGTGCAGAGATCAAACTTGGGAAGAATCGGACAGAGGCGGAAGTAAAGCGGTACACGGAGGAGAAGGAGA GGCTGGAGAGGAGCAAGGAGGAGATCCGAGGGCACCTGGCTCAGCTCCGCAGAGAGAAGCGGGAGCTCAA AGAGACCCTGTTGAGATGCACAGATAAGGGGGTCCTGGCCAAGCTGGAGCAGACACTGAAGAAAATAGAC GAGGAATGCCGGATGGAGGAGAGCAGGCGTGTGGACCTTGAGCTCAGCATCATGGAGGTGAAGGACAACC TGAAGAAGGCAGAGGCTGGGCCCGTGACCCTGGGCACCACTGTGGATACCACGCACCTGGACAACATGAG CCCTCGTCCACAGCCCAAAGCTGCCACCCCCAACCCCCCACCAGACTCCACGCCAGTCAACTCTGCATCT GTGCTCAAGAACAGGCCTCTTTCCGTCATGGTCACAGGCAAAGGCACTGTCCTGCAGAAAGCCAAGGAAT GGGAGAAGAAAGGAGCCAGTTAGGAGACAAGAATTGCCTAAAGACTCTTATGTGACTGTGGACCTTGGTG ACAATCCTGCTCTGGACAAGTCTTCAATAAAACAGCAACTCTGAGGAAAGGTGAGTCTGTTTACAAGACA AAGCAAGGGATGAGGCGCTCGCTGCAAACGGAGCTTCGGTTAAGAGAAGGCCCTGTCTCCTCCTCAAGCC AAAGGGATCCCAGCAGCAGCATCGTGGTGGCATCTTTGGAGTCGAAATCTACTGGCTGTCAATGAGACAA TGTACCTTCCCTTTTCTTTTTAAAGATTTCATCCATCACTCAACTGTTTTACCAAGATTTTTCAGGTGTG AAAAAATGACCACATTGCTTCTGGTTATGAGAAAAATCTAAATTCCTGACCCCCTTATCTTTCATCCTGT ATTTGGCAATGTTGAGGTTTAAATCATGCATTGTCTTCATGTTTGCCCAAATTGGGTGGGCTCCATGTGG TCTCTGATGGTCTTAAAGAACATTAGAACCCTGTAATCAGTAGAAGAGGGACATATTGGGAACCAAGCCA AGGCACTGACCCCCTTGGGGTCACTAAGGACTTCCCCCCAGTCCAGGAAAACTTTAAACAATGCTGTATT ATATAGGGGGAAAATTCCTGTGTTTACAGACTGTCCTGTGGCCAGGCCACTTTTAAGAGTTGGTCAATTA CCTCTTCATTACCATTGTAAAGATGTTTTAAATTTCTGGTCCCCTTGCAAGGAAAACAGCCGTGAATTAG CCTGTCTAGTCTGTCAGACTGGCAGCCAGTGCTGTCTTTATGTGCTGTCTCTCTGGGACAGCACTAGCAC AAGTTGATACATTTCATCTGTTACCTGCCCCATTTCTGAGCATTTGGAACTAGTTACCTAACACTGGTTT CTGAAGGCTAAATGTTTTTACGCATATATTATTGTCTTTCTAATCCCAGGGCATTTATTTCCTGTATCAT AAACACTTGGTGACCTTTATCAAGTGGTGAGTTAGAATTTTTTTTTTAAATAAAATGTTCATTGTAAAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3711746 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3711746 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11218 same embryo
	EMAGE:11215 same embryo
	EMAGE:11217 same embryo
	EurExpress:euxassay_003397 same experiment
	MGI:4823020 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source