EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11224

Gpr89 G protein-coupled receptor 89 ( MGI:1914799)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003481. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003481_01	euxassay_003481_02	euxassay_003481_03	euxassay_003481_04

euxassay_003481_05	euxassay_003481_06	euxassay_003481_07	euxassay_003481_08	euxassay_003481_09

euxassay_003481_10	euxassay_003481_11	euxassay_003481_12	euxassay_003481_13	euxassay_003481_14

euxassay_003481_15	euxassay_003481_16	euxassay_003481_17	euxassay_003481_18	euxassay_003481_19

euxassay_003481_20	euxassay_003481_21	euxassay_003481_22	euxassay_003481_23	euxassay_003481_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11224_wholemount_strong.wlz

11224_wholemount_moderate.wlz

11224_wholemount_weak.wlz

11224_wholemount_possible.wlz

11224_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11224_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 13 14 15 weak expression: see section 12

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 17 18 19 weak expression: see section 10 11

pancreas

moderate

regional

moderate expression: see section 10 11 12 13 17 18

vibrissa

moderate

regional

moderate expression: see section 06 weak expression: see section 07 08 21 22

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22 23

ventral grey horn

moderate

regional

moderate expression: see section 16 17 weak expression: see section 13 14 15

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 17 18 19

stomach

weak

regional

weak expression: see section 06 07 08 09 10

hindgut

moderate

regional

moderate expression: see section 13 14 15 16

midgut

moderate

regional

moderate expression: see section 13 14 15 16 17 18 weak expression: see section 06 07 08 09 10 11 12

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 not examined expression: see section 13 14

liver right lobe

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 23 24

clavicle

weak

regional

weak expression: see section 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6783
Entity Detected:	Gpr89, G protein-coupled receptor 89 ( MGI:1914799)
Sequence:	sense strand is shown >T6783 GGACAGGTCTTAAGCCGCGGCTTCTCTGAGGGGTAGACAGCTGGGCATGGGCCTCCCGAGGCTTCGCCAT GAGCTTCCTGATCGACTCCAGCATCATGATCACCTCCCAGATACTGTTTTTTGGATTCGGATGGCTTTTC TTCATGCGCCAGTTGTTTAAGGACTATGAGGTGCGTCAGTATGTGGTGCAGGTGATCTTCTCCGTGACCT TTGCATTTTCATGCACCATGTTTGAGCTTATCATCTTTGAGATCTTGGGAGTTCTGAACAGCAGCTCCCG TTACTTCCATTGGAAACTGAACCTGTGTGTGATTCTGCTCATCCTGGTTTTCATGGTGCCCTTCTACATT GGTTACTTTATCGTGAGTAACATCCAGCTGTTGCACAAACAGCGCCTGCTCTTTTCCTGTCTCTTATGGC TGACCTTTATGTACTTCTTCTGGAAGCTGGGAGACCCATTCCCCATACTCAGCCCAAAGCACGGGATCTT GTCCATAGAGCAGCTCATCAGCCGGGTAGGCGTAATCGGGGTGACGCTCATGGCTCTGCTTTCCGGATTT GGTGCTGTCAACTGTCCGTACACATACATGTCCTACTTCCTCAGGAACGTGACTGACACAGATATTCTTG CCCTGGAACGGCGACTGCTGCAGACCATGGACATGATCATAAGCAAAAAGAAAAGAATGGCCGTGGCACG GAGAACCATGTTCCAGAGAGGGGATGTGCAGAACAAGCCGTCGGGACTCTGGGGCATGCTGAAGAGCGTT ACTGCCTCAGCCCCGGGAAGTGAAAATCTGACTCTTATCCAACAGGAAGTGGATGCTTTGGAAGAACTGA GCAGGCAGCTTTTTCTGGAAACAGCTGATCTGTATGCTACAAAGGAAAGAATTGAATACTCGAAAACATT CAAGGGAAAGTATTTTAATTTCCTGGGTTATTTTTTCTCTATTTACTGTGTCTGGAAAATTTTTATGGCT ACCATCAATATTGTTCTTGATCGAGTTGGGAAAACTGACCCTGTCACCAGAGGCATCGAGATCACTGTGA ATTATCTGGGAATCCAGTTTGACGTGAAGTTCTGGTCTCAGCACATCTCCTTCATTCTGGTTGGAATAAT CATTGTCACATCCATCAGAGGACTGCTGATCACACTCACCAAGTTCTTTTATGCCATCTCCAGCAGCAAG TCCTCCAACGTCATCGTCCTGCTGTTAGCGCAGATAATGGGTATGTACTTCGTGTCCTCTGTACTGCTGA TCCGAATGAGCATGCCCCCGGAGTACCGCACCATAATCACAGAGGTCCTCGGGGAGCTTCAGTTCAACTT CTATCACCGTTGGTTTGATGTCATCTTCCTGGTCAGTGCCCTCTCCAGCATATTGTTCCTCTATCTGGCT CACAAACAGGCACCAGAGAAGCATATGGCCCCCTGAACTCAGACTCCCTGCCAACTTCAGGAAAGGAGAC ACAGTGGGGAGACCAGAGCCTACAGCTCTTTACACAGGCAGAGCTGCGAGTTCTGTTCTGCCTCAGGCAC ACGCCTACTTCCTCCTGAGATACTGGAGTCACTTCGACTGAGGCACAAGAAGAAAACCCGCAGCATTCAG CAGGGAGCCGCATGGATCTGAGGCTGTGCAGTGAGGAGGAGAGGGCCAGAGCATGAGGATACCCCGGAAA TCCTGACTACACGGCTTCCCTCGAAAATGCTAGTAAAGTCAGTTTGTAACACTCTGAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:3708143 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3708143 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11225 same embryo
	EMAGE:11226 same embryo
	EMAGE:11227 same embryo
	EurExpress:euxassay_003481 same experiment
	MGI:4825196 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source