EMAGE mouse gene expression spatial database

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EMAGE:11275

Stim1 stromal interaction molecule 1 ( MGI:107476)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006651. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006651_01	euxassay_006651_02	euxassay_006651_03	euxassay_006651_04

euxassay_006651_05	euxassay_006651_06	euxassay_006651_07	euxassay_006651_08	euxassay_006651_09

euxassay_006651_10	euxassay_006651_11	euxassay_006651_12	euxassay_006651_13	euxassay_006651_14

euxassay_006651_15	euxassay_006651_16	euxassay_006651_17	euxassay_006651_18	euxassay_006651_19

euxassay_006651_20	euxassay_006651_21	euxassay_006651_22	euxassay_006651_23	euxassay_006651_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11275_wholemount_strong.wlz

11275_wholemount_moderate.wlz

11275_wholemount_weak.wlz

11275_wholemount_possible.wlz

11275_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11275_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 16 17 18 19 20 21 22 23 24

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 06 07 08 09 10 11 13 14 16 17 18 19 20

4th ventricle lateral recess

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 15 weak expression: see section 08 16

pons mantle layer

moderate

regional

moderate expression: see section 07 17

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22 23

ventral grey horn

moderate

regional

moderate expression: see section 09 10 11 12 14 15 16 weak expression: see section 13

tongue

weak

regional

weak expression: see section 17

tongue muscle

weak

regional

weak expression: see section 11 12 13 14 15 16 17

mandible

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 17 18 19 20 21 22 23

maxilla

moderate

regional

moderate expression: see section 06 07 08 09 17 18 19 20 21 22

ovary

moderate

regional

moderate expression: see section 05 06 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7342
Entity Detected:	Stim1, stromal interaction molecule 1 ( MGI:107476)
Sequence:	sense strand is shown >T7342 CCACGCGTCCGGGCCCGTGCGCGTCCGCCTGCTGCACCGGGGCACCAGGAGCCGCAGAGGTACCGGACCC GGCGGGGGCGCTGACCTCGGCCTAGGAGTCTTAGGATCCCGGAGACATCCGTGTCTGCTGGGGCCTGTAG GTCGCACGCCGAAGCCCTAGCTGCTGAGGCTCACCGCTGTTTCGCGGGGCGAGGTCAGGTGCCCCCCTTC TCTCCTCTCTTCTCTCCCTCCCCCACCTCAGTGCGGGCGGGAGATTCTGCCCGCCTCCTCCCGCAGGGGT GCAGCAGGCTGCGGAGCTGACAGCGGCCCCGCAGCCACCCTGCTCAAACTCTCCGGAAGCAGATAGAGCT CAGGCCGCCGCCGCAGCCCCGGCGGACCTACAGTTGGACCGGAGACTCCGATCCTTCTGCGTCCAAACTT GGGGCACTTGACCTTCGCTTATCGGAGGAGGTAAGCCCGCAGGTGGCTGGACAGCTGCGGCGCCGCGAGG GCATCTTGCTTTGGAACCGTCGGCTGCACTCCCTGACTCTGGGATTTGCTTCTGGGATCCAAAGGTGTCT ACAGCAGGCGCATGTTGACTGAGACCTACCGTCATGGATGTGTGCGCCCGTCTTGCCCTGTGGCTTCTTT GGGGGCTCCTTCTGCATCAGGGCCAGAGTCTCAGCCATAGTCACAGTGAAAAGAATACAGGAGCTAGCTC CGGGGCGACTTCTGAAGAGTCTACCGAAGCAGAGTTTTGCCGAATTGACAAGCCCCTGTGCCACAGTGAG GATGAGAAGCTCAGCTTTGAGGCCGTCCGAAACATCCATAAGCTGATGGATGACGATGCCAATGGTGATG TGGATGTGGAAGAAAGTGATGAGTTCCTAAGGGAAGACCTCAATTACCATGACCCAACAGTGAAACATAG CACCTTCCATGGTGAGGATAAGCTTATCAGCGTGGAGGACCTGTGGAAGGCGTGGAAATCATCAGAAGTG TACAACTGGACTGTGGATGAGGTGATACAGTGGCTCATTACGTATGTGGAGCTGCCACAGTATGAGGAGA CCTTCCGGAAGTTGCAGCTTACTGGCCACGCCATGCCAAGGCTAGCAGTAACCAACACCACCATGACAGG GACTGTACTGAAGATGACAGATCGGAGCCACAGGCAGAAGCTGCAGCTGAAGGCCCTGGACACAGTGCTG TTTGGGCCTCCTCTCTTGACTCGGCATAATCACCTGAAGGACTTCATGCTGGTGGTGTCTATCGTTATTG GTGTGGGTGGCTGCTGGTTTGCCTATATCCAGAACCGTTACTCTAAGGAGCACATGAAGAAAATGATGAA GGATCTGGAAGGGTTACACCGGGCTGAGCAGAGTCTGCATGACCTTCAGGAAAGGCTGCACAAGGCCCAG GAGGAGCACCGAACTGTGGAAGTAGAGAAAGTCCACCTGGAGAAGAAGCTGCGAGATGAGATCAACCTTG CCAAGCAGGAAGCTCAGCGGCTGAAGGAGCTGAGGGAGGGTACTGAGAATGAGAGGAGCCGTCAAAAATA TGCTGAGGAAGAGCTGGAGCAGGTTCGGGAGGCCTTGAGGAAAGCAGAGAAGGAGCTGGAATCACACAGC TCATGGTATGCTCCTGAGGCCCTGCAGAAGTGGCTGCAGCTGACCCATGAGGTGGAGGTGCAGTACTACA ACATCAAGAAGCAAAATGCAGAGAGGCAGCTGCTGGTGGCCAAGGAGGGGGCTGAGAAAATAAAAAAGAA GAGAAACACGCTTTTTGGTACCTTCCATGTGGCCCACAGCTCTTCCCTGGATGATGTGGATCATAAAATC CTAACTGCTAAGCAAGCTCTGAGTGAGGTGACAGCGGCACTGAGGGAGCGCCTGCACCGGTGGCAGCAGA TCGAGATCCTCTGCGGTTTCCAGATTGTCAATAACCCCGGCATCCACTCCTTGGTGGCTGCTCTCAACAT CGACCCCAGCTGGATGGGCAGCACCCGCCCTAACCCCGCCCACTTCATCATGACTGACGATGTGGATGAC ATGGATGAGGAGATTGTGTCGCCCTTGTCCATGCAGTCCCCCAGCCTGCAGAGCAGTGTCCGGCAGCGCC TGACGGAGCCACAGCTTGGCCTGGGATCTCAGAGGGATTTGACCCATTCCGATTCGGAGTCCTCCCTCCA CATGAGTGACCGCCAGCGTGTGGCCCCCAAGCCTCCTCAGATGGGCCGTGCTGCAGATGAAGCTCTCAAT GCCATGCCTTCCAATGGCAGCCATCGGCTGATTGAGGGGGTCCATCCAGGATCTCTGGTGGAGAAACTGC CTGACAGCCCTGCTCTGGCCAAGAAGACATTTATGGCGTTGAACCATGGCCTAGACAAGGCCCACAGCCT GATGGAGCTGAACCCCTCAGTCCCACCTGGTGGCTCCCCACTTTTGGATTCTTCCCATTCTCTTAGCCCC AGTTCCCCAGACCCAGACACACCATCTCCAGTTGGGGACAACCGAGCTCTGCAGGGTAGCCGAAACACAC GAATTCCCCACTTGGCTGGCAAGAAGGCAATGGCTGAGGAGGATAATGGTTCCATTGGTGAGGAGACAGA CTCCAGTCCAGGCAGGAAGAAGTTTCCTCTCAAAATTTTTAAGAAGCCTCTTAAGAAGTAGGCAGACTAG GGTGGTAGTGTTGAGACAGCCTGTCCTTCCCTGGGTCTTCTGCCTTCACCTCCCTTCCTTTCTTTGCAAT ATCTGGCTCCTAGAGTGGGGCACAGAGGGGCTGGCCCAAGGGCCTGGGCACTGTACATATCTGCCCTGCT CATCCTTGGTCCTTCATCATTATTTATTAACTGACCACCATGGCCTGCCTGTCGGGAAACCCTTCCACCC ATGGGCTGCTGCTGTCACATCTTCTCCACTTCAGTGCATGTCTTAGTTGCTCTTCCCTCAGTTCCCACTC CACTTTTGGGGTCCAGCTTCTGTCTCTGCTGTCCCAGTTTTGAGGTTTGGTTTTTGTTTCTGTCTCCTGC TTTCAGGCTCCTCTCTCCCATTACTCCCCAACGATCCTAGCAGTTGTGGGGAAGATAGGAGGAGTAACTT CTGACACCTGTACCTCAGATCTGTTCATCCTACTCACAGCCATTCTGCCTACCCCAGACTGGGCCACGGT CCTGATCTCTGGGGTTTGTTCTATGGAAGTGTGGTAGACTGGAGGGAACCTCATCCTGGAGCTCCTTTGG ATCTCCAGGGCTCCATTCAGGGAGTGGAACCAACTCCCAGGGAACAAGTCACCAGAGTTTTAAAGAGAGA CCAGGCTTGTGATTGATGGGAGAGACTTCTCCCAGTTGGAGGATGAGTAGATGCCAAAGCTGTGGGCTGT AAGGCAGTTGCCATTTCTCTCTTGCCCTGCCCACACCTGCCTTCCTCTTACCTCTGCTCCCCTATATTGC AGGAGTGTATCTCTTAAGAGGTGCTGCCCTGAAGCTCCCCATCAGCATCAGCACTACTGGGGCTCAGGGC AACTGGCTCCCCTGGCTATGGGAGCCACAGTCATGACACAGGGCTCTTGTGGAGCCCTGGGCAAGGATGT TATATTTGAACCAAAAGACAAACAGTTTTAAAATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4191156 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4191156 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11276 same embryo
	EMAGE:11277 same embryo
	EurExpress:euxassay_006651 same experiment
	MGI:4828498 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source