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EMAGE:11280

Ncaph2 non-SMC condensin II complex, subunit H2 ( MGI:1289164)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11280 EMAGE:11280 EMAGE:11280 EMAGE:11280 EMAGE:11280
"Pseudo-wholemount" of euxassay_006527. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006527_01 euxassay_006527_02 euxassay_006527_03 euxassay_006527_04
EMAGE:11280 EMAGE:11280 EMAGE:11280 EMAGE:11280 EMAGE:11280
euxassay_006527_05 euxassay_006527_06 euxassay_006527_07 euxassay_006527_08 euxassay_006527_09
EMAGE:11280 EMAGE:11280 EMAGE:11280 EMAGE:11280 EMAGE:11280
euxassay_006527_10 euxassay_006527_11 euxassay_006527_12 euxassay_006527_13 euxassay_006527_14
EMAGE:11280 EMAGE:11280 EMAGE:11280 EMAGE:11280 EMAGE:11280
euxassay_006527_15 euxassay_006527_16 euxassay_006527_17 euxassay_006527_18 euxassay_006527_19
EMAGE:11280 EMAGE:11280 EMAGE:11280 EMAGE:11280
euxassay_006527_20 euxassay_006527_21 euxassay_006527_22 euxassay_006527_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11280Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11280_wholemount_strong.wlz
11280_wholemount_moderate.wlz
11280_wholemount_weak.wlz
11280_wholemount_possible.wlz
11280_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11280_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
embryo
strong strong
ubiquitousstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6669
Entity Detected:Ncaph2, non-SMC condensin II complex, subunit H2 ( MGI:1289164)
Sequence:sense strand is shown

>T6669
GCCAGAACCAGTAGCGGGTTGAGCCATGGTTCCGGGCTTCTAGTCCCGTTCTAGACATGGAGGATGTGGA
GGTGCGCTTTGCTCACCTCTTGCAGCCCATCCGGGATCTCACTAAGAACTGGGAGGTGGACGTGGCGGCA
CAGCTGGGCGAGTATCTGGAGGAGCTGGACCAGATCTGCATTTCTTTTGATGAAGGCAAAACCACAATGA
ACTTCATTGAGGCAGCACTGTTGATCCAGGGCTCAGCCTGTGTCTACAGTAAGAAGGTGGAGTACCTCTA
CTCGCTGGTCTACCAGGCTCTCGATTTTATTTCTGGCAAGAGGCGGGCCAAACAGCTCTCCTTAGTTCAG
GAAGATGGGAGCAAGAAGACTGTCAACTCAGAGACTCCCTGTGAAACAGAGAATGAGTTCCTGTCACTGG
ACGACTTCCCTGACTCCCGGGCTAATGTGGATCTGAAAAATGATCAGGCATCCAGTGAGCTGCTTATCAT
ACCCCTACTGCCCATGGCCCTGGTGGCCCCTGATGAAGTGGAAAAGAACAGCAGCCCCTTGTATAGCTGT
CAGGGTGACATCTTGGCCAGCCGGAAGGATTTCAGGATGAACACGTGTATGCCTAACCCCAGAGGCTGCT
TTATGTTAGATCCAGTGGGAATGTGTCCTGTGGAGCCTGTGGTGCCCGTGGAGCCATACCCCATGTCAAG
GAGCCAGAAAGATCCTGAGGACGCTGAGGAGCAGCCCATGGAAGTGTCTAGGAACGGGAGTCCTGTTCCT
GTACCCGACATCTCCCAAGAGCCAGATGGTCCAGCGCTCAGCGGTGGAGAGGAGGATGCAGAGGATGGAG
CAGAGCCCCTGGAGGTTGCTCTAGAGCCTGCAGAGCCAAGGACCTCACAGCAGAGTGCCATCTTGCCAAG
GAGATACATGCTGCGGGAACGACAAGGGGCACCGGAGCCTGCCTCCCGGCTACAGGAGACCCCAGACCCC
TGGCAGAGCCTGGACCCTTTTGACTCCTTGGAATCTAAGGTCTTCCAGAAAGGGAAACCCTATTCTGTGC
CACCCGGTGTGGAGGAGGCTCCAGGACAGAAGCGCAAGAGGAAGGGTGCCACCAAGTTGCAGGACTTCCA
CAAGTGGTACCTGGATGCCTATGCTGAACACCCTGACGGCAGGAGGGCTCGGCGGAAGGGCCCAACCTTT
GCAGACATGGAAGTCCTGTACTGGAAACATGTGAAAGAACAGCTCGAGACCCTTCAGAAGCTGCGGAGAC
GTAAGATCAACGAGAGATGGCTACCTGGGGCCAAGCAGGATCTGTGGCCTACAGAGGAGGATCGCTTGGA
AGAGTCCCTCGAGGACCTCGGGGTAGCAGATGACTTTCTAGAGCCCGAGGAGTACGTGGAGGAGCCTGCG
GGGGTGATGCCCGAGGAAGCTGCTGACCTCGATGCAGAGGCCATGCCAGAGTCCCTGAGATACGAGGAGC
TGGTCCGGAGAAATGTGGAACTCTTCATTGCCACCTCCCAGAAGTTTATCCAGGAGACAGAGCTGAGCCA
ACGCATCAGGGACTGGGAAGATACCATCCAGCCCCTGCTCCAGGAGCAGGAGCAGCATGTGCCCTTTGAT
ATCCATATCTACGGGGACCAGTTGGCTTCACGGTTCCCCCAGCTCAATGAATGGTGTCCCTTTTCAGAGC
TTGTAGCAGGGCAGCCTGCTTTTGAGGTGTGCCGCTCCATGCTGGCCTCCTTGCAACTGGCTAATGACTA
CACAGTGGAGATCACTCAGCAGCCAGGACTGGAGGCAGCTGTGGACACAATGTCTCTGAGACTGCTCACA
CACCAGCGAGCCCACACCCGCTTCCAGACCTATGCTGCACCATCCATGGCCCAGCCTTGAGTGGACAGCA
CTGAGGCAGGGGTGGAAAGTAGTATATACCTGGAGGTCTTTGCCCCTAATGTGCTATGGGGCCATTCACT
CCAGTGCTGCCTCCTGGCTGGCCTAGCCTAATAAAGTGTTGCTACCCCAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3601298 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3601298 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:MGI:4826614 same experiment
 EurExpress:euxassay_006527 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS