EMAGE mouse gene expression spatial database

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EMAGE:11336

Serpinb5 serine (or cysteine) peptidase inhibitor, clade B, member 5 ( MGI:109579)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006553. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006553_01	euxassay_006553_02	euxassay_006553_03	euxassay_006553_04

euxassay_006553_05	euxassay_006553_06	euxassay_006553_07	euxassay_006553_08	euxassay_006553_09

euxassay_006553_10	euxassay_006553_11	euxassay_006553_12	euxassay_006553_13	euxassay_006553_14

euxassay_006553_15	euxassay_006553_16	euxassay_006553_17	euxassay_006553_18	euxassay_006553_19

euxassay_006553_20	euxassay_006553_21	euxassay_006553_22	euxassay_006553_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11336_wholemount_strong.wlz

11336_wholemount_moderate.wlz

11336_wholemount_weak.wlz

11336_wholemount_possible.wlz

11336_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11336_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

spotted

moderate expression: see section 13 14 15 weak expression: see section 03 04 05 06 07 08 09 10 11 12

vertebral axis musculature

weak

spotted

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

epidermis

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vibrissa

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 20 21 22 23

inner ear

strong

regional

strong expression: see section 05 06 07 08 17 18 19

pharyngo-tympanic tube

strong

regional

strong expression: see section 04 05 06 07 17 18 19 moderate expression: see section 01 02 03 08 09 10 16 20 21 22 23

anterior naris epithelium

strong

regional

strong expression: see section 13 14 16 17

external naris epithelium

strong

regional

strong expression: see section 12 13 18

naso-lacrimal duct

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 19 20 21 22 23

heart ventricle

moderate

spotted

moderate expression: see section 09 10 11 12 13 14 15 17 18 weak expression: see section 07 08 16

esophagus epithelium

strong

regional

strong expression: see section 12 13

pharynx epithelium

strong

regional

strong expression: see section 10 11 12 13 14 15

tongue epithelium

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18

stomach proventricular region epithelium

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11

lower jaw incisor

strong

regional

strong expression: see section 10 12 13 16 18 moderate expression: see section 11

lower jaw molar

strong

regional

strong expression: see section 07 08 09 17 19 20

oral epithelium

strong

regional

strong expression: see section 21 moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 22

upper jaw incisor

strong

regional

strong expression: see section 13 16

upper jaw molar

strong

regional

strong expression: see section 07 17 20

bladder

moderate

regional

moderate expression: see section 14 15

urethra of male

moderate

regional

moderate expression: see section 14 15

lung

strong

spotted

strong expression: see section 08 09 16 17 18 moderate expression: see section 03 04 05 06 07 10 11 12 13 14 15 19 20 21 22 23

trachea

moderate

spotted

moderate expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6654
Entity Detected:	Serpinb5, serine (or cysteine) peptidase inhibitor, clade B, member 5 ( MGI:109579)
Sequence:	sense strand is shown >T6654 GCACAGCTTTCACTGCCTCTGAGCTCCTTCGGCTTTTTTCCCCCCTTTTGCATCTTGTTTCCCGGGATAC CTGCAACTCAAGGATGGATGCCCTGAGACTGGCAAATTCAGCTTTTGCTGTTGACTTGTTCAAACAACTA TGTGAAAGGGACCCAGCAGGAAACATTCTCTTCTCTCCAATATGCCTCTCTACTTCTCTGTCCCTTGCGC AAGTGGGCACCAAAGGCGACACAGCAAATGAAATTGGACAGGTCCTTCATTTTGAGAATGTCAAAGATGT ACCCTTTGGGTTTCAAACAGTCACTTCTGATGTTAATAAGCTCAGTTCTTTTTACTCTTTGAAACTTGTC AAGCGACTCTACATAGACAAATCCCTGAACCCTTCTACAGAATTTATCAGTTCTACCAAAAGACCATATG CAAAAGAATTGGAAACTGTTGACTTCAAAGACAAACTGGAAGAAACGAAAGGTCAAATTAACAGCTCCAT TAAGGAGCTCACAGATGGCCACTTTGAGGACATTTTGTCAGAGAACAGTATAAGTGACCAGACCAAAATC CTTGTGGTTAATGCTGCCTACTTTGTTGGAAAGTGGATGAAGAAATTTCCGGAATCAGAAACAAAAGAAT GTCCTTTCAGAATCAGCAAGACAGACACCAAACCCGTACAAATGATGAATCTTGAGGCCACTTTCTGCTT GGGTAACATTGATGACATCAGCTGTAAGATCATAGAACTTCCTTTCCAGAATAAGCATCTGAGTATGCTC ATTGTGCTCCCCAAGGACGTGGAGGATGAGTCCACAGGCCTGGAGAAGATTGAACAGCAACTCAACCCAG AAACATTGTTACAGTGGACCAACCCCAGTACCATGGCCAATGCCAAAGTCAAACTTTCCCTCCCAAAGTT TAAGGTAGAAAAGATGATTGATCCCAAGGCTAGTCTGGAAAGCCTAGGGCTGAAAAGTCTCTTCAATGAA AGTACATCGGATTTCTCTGGAATGTCAGAGACCAAGGGAGTGTCCCTGTCAAATGTGATTCATAGAGTAT GCCTAGAAATAACCGAAGATGGTGGTGAGTCCATCGAGGTGCCAGGGTCCCGGATCTTACAGCACAAGGA TGAATTCAATGCTGACCATCCATTTATTTATATCATTAGACACAACAAAACTCGAAACATCATTTTCTTT GGCAAATTCTGTTCTCCTTAGCTGGCAGGGCCTTGCCAAGTCTCAGGGAACTTGTCTGTAGTCGCAGAGC TCTGTAAACTTTGTATCCAGACAATCACTTTCTATACAATAAATTGTAAATGTTGCTGAATCAGGAAGCA GTCCACATTTGTCATATGTAACCTTCACACTAACAGACACCCCTTTTCCTAATTCTTCCTTGTTTTCCTT TGTCAAAGAAAACAGCGAATACTTGTTGGGAAAGGGAATCACTTTAGAAAATATGTGCTCTTCATTTGTC AAAGCATTCAGAGCTATGGATAAAAATGCAGTCTTCAAGGGAGGAACTTCTATAGGGATGATGTGGGGGA CATTATTCTCAGGGCTCTGCATTACTGAGATAAAGAATGTTCTAGAGATCTCCACTTCCTTCAACTCTTA AGAACAAGAGTATAGGGTTCACCAAGTACCTTTTTAAAGTGAATTTGTGTACATGTCACAATACTGTAGG TCTAGAAGTCTCCATGTTAAACCTTAAGAAACAGTTTTCTTCAATATCCTAATTGGAGATTATAGAAAGA TGACATGTTGTAAATACTATATATCATGGAACTCCACAAATAGCATATGGACTATCACCCTATCAACCAA TACCTTAATATTTGTTCCATAAATATAGACTATTCACACAAAACAAGTACATAAAGATAGTTCATAAGTG ATCAATTTTTTCCCTGAAAAATGAGTATGTTATCAGCTCATGGAAACACTTTGAAGAACATTCTAGATCT TGGAGTGTTGGATAAAGAATTCTGGGCCTATTTTAGCTGAAGACTAAGACCCTAAGGGAAAGCTCAGCTT TTACAAAAGTTGGCTTTCATTTTTCCTAGATCTCCCATGTGGTCATTTGGGCAATGTTGTTGCTGGCAGG CTTCTAACCAGATCACAAGGTCCCTGACATCTCCAGCGCCAGCAACTTAAAATCTACAGGAAGTCTCGTG ATGCTAAGAGTTTTGTGGCCATTAAAGGAACTTTCCTTTTTTGTCTAATGCGGCAGTGGAGAAAGAGAAG GGATCTCAAACCTTCAGAAAATACAAGCAACACTATTTAGGTACACATAGTTCTTTGGCATATATTTTCT CAGTTTACAAACCTAACGACTTCAGATAACAGTGTGTAGTCTACATAAATAATTTATAAAATGGTTCTGG TAAGTCAGTTATGTTGCAACCTAATTTTAAAGTTGCCACCAAAAGGAAGTATTTGCTGCAGTTTTTCTTA TGTGTCTTTCCTTACCCAATTTCTTGCCATTTATTGTAAATAGGGTTTTTTTTTCCTCCAAAGGTTCAAT GGTGACTTCTTTTCTGTGCTATTGATAATAAAATATTATTGAATTAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3600282 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3600282 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11335 same embryo
	EurExpress:euxassay_006553 same experiment
	MGI:4827992 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source