EMAGE mouse gene expression spatial database

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EMAGE:11355

Pfkl phosphofructokinase, liver, B-type ( MGI:97547)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006644. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006644_01	euxassay_006644_02	euxassay_006644_03	euxassay_006644_04

euxassay_006644_05	euxassay_006644_06	euxassay_006644_07	euxassay_006644_08	euxassay_006644_09

euxassay_006644_10	euxassay_006644_11	euxassay_006644_12	euxassay_006644_13	euxassay_006644_14

euxassay_006644_15	euxassay_006644_16	euxassay_006644_17	euxassay_006644_18	euxassay_006644_19

euxassay_006644_20	euxassay_006644_21	euxassay_006644_22	euxassay_006644_23	euxassay_006644_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11355_wholemount_strong.wlz

11355_wholemount_moderate.wlz

11355_wholemount_weak.wlz

11355_wholemount_possible.wlz

11355_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11355_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 02 03 04 05 19 20 21 22 23 24 moderate expression: see section 01 06 07 08 18

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

thymus primordium

moderate

regional

moderate expression: see section 11 12 13 14 15

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 16 17 18 19

pituitary gland

strong

regional

strong expression: see section 10 11 12 13 14 15

vibrissa

strong

regional

strong expression: see section 04 24 moderate expression: see section 05 06 07 08 09 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 15 16 17 18 19 20

heart left ventricle

moderate

regional

moderate expression: see section 06 07 08 09 10 11

heart right ventricle

moderate

regional

moderate expression: see section 12 13 14 15 16 17

esophagus

moderate

regional

moderate expression: see section 12 13

stomach

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09

hindgut

strong

regional

strong expression: see section 15 16

rectum

strong

regional

strong expression: see section 15 16

midgut

strong

regional

strong expression: see section 12 13 14 15 16 17 18

midgut loop

strong

regional

strong expression: see section 10 11 12 13 19 20

lower jaw incisor

strong

regional

strong expression: see section 12 14 16 17

lower jaw molar

strong

regional

strong expression: see section 07 08 09 18 20

upper jaw incisor

strong

regional

strong expression: see section 12 13 16 17

upper jaw molar

strong

regional

strong expression: see section 07 08 09 18 20

trachea

moderate

regional

moderate expression: see section 13 14

axial skeleton

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

basioccipital bone

moderate

regional

moderate expression: see section 01 02 03 04 05 19 20 21 22 23 24

temporal bone

moderate

regional

moderate expression: see section 01 02 03 04 21 22 23 24

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 19 20 21 22 23 24

clavicle

strong

regional

strong expression: see section 09 10 16 17 18

axial skeleton tail region

moderate

regional

moderate expression: see section 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7085
Entity Detected:	Pfkl, phosphofructokinase, liver, B-type ( MGI:97547)
Sequence:	sense strand is shown >T7085 CGGACGCGTGGGCGCGGAACGCAGAGCGGCGGGAGAGGAGCTTGGGCTCCTGGTCTCCGCTGCCGCCATG GCTACCGTGGACCTGGAGAAACTGCGGATGTCGGGGGCTGGCAAGGCCATTGGAGTGCTGACCAGCGGCG GTGATGCGCAAGGTATGAATGCTGCTGTCAGGGCTGTGACCCGTATGGGCATATATGTGGGGGCCAAAGT CTTCCTCATCTATGAGGGCTACGAGGGCCTTGTGGAAGGAGGCGAGAACATCAAGCCAGCCAACTGGCTC AGCGTTTCCAATATCATCCAGCTGGGTGGCACCATTATTGGCAGTGCCCGCTGTAAGGCCTTCACTACGA GGGAAGGCCGCCTGGCTGCAGCCTACAATCTGCTCCAACACGGCATCACCAACCTGTGTGTCATCGGTGG CGATGGCAGCCTCACGGGGGCCAACATCTTCCGCAACGAGTGGGGCAGCTTGCTGGAGGAGCTGGTGAAG GAAGGCAAGATCTCAGAGTCCACAGCTCAGAACTACGCACACTTGACCATCGCCGGTCTGGTGGGCTCCA TCGATAATGACTTCTGCGGCACTGACATGACCATTGGCACAGACTCAGCCCTGCACCGCATTATGGAGGT CATTGACGCCATCACTACCACTGCCCAAAGTCACCAGAGGACCTTTGTTTTGGAGGTGATGGGACGGCAC TGCGGGTACCTGGCGCTGGTGTCTGCGCTGGCTTCCGGGGCTGATTGGCTATTCATTCCTGAAGCGCCCC CTGAGGATGGCTGGGAGAACTTCATGTGTGAGAGGCTGGGCGAGACTCGGAGCCGAGGCTCTCGGCTGAA CATCATCATCATCGCAGAGGGCGCCATTGACCGGCATGGAAAGCCTATCTCATCCAGCTACGTGAAGGAT CTGGTGGTTCAGAGGCTGGGCTTCGATACACGAGTGACTGTGCTGGGTCATGTACAGCGAGGAGGGACGC CTTCAGCCTTCGACCGAATCCTGAGTAGCAAGATGGGTATGGAGGCCGTGATGGCGCTGCTAGAGGCCAC GCCTGACACGCCGGCCTGTGTGGTCAGCCTCTCCGGGAACCAGTCTGTGAGGCTGCCTCTCATGGAGTGT GTGCAAGTGACAAAGGACGTGCAGAAGGCCATGGACGAGGAGAGGTTTGACGAGGCCATCCAGCTCCGTG GCAGGAGCTTTGAGAACAACTGGAAAATTTACAAGCTCCTTGCCCACCAGAAGGTGTCTAAAGAGAAGTC CAACTTCTCCCTGGCCATCCTGAATGTGGGGGCTCCAGCTGCTGGCATGAATGCAGCTGTGCGCTCCGCA GTGCGCACCGGTATCTCCGAGGGACACACGGTATACATCGTGCATGATGGCTTTGAGGGTCTGGCCAAGG GTCAGGTGCAAGAAGTGGGCTGGCATGATGTGGCAGGCTGGCTGGGACGTGGTGGCTCGATGCTGGGGAC CAAGAGGACACTGCCCAAGCCCCATCTGGAGGCCATTGTGGAGAATCTCCGCACCTACAACATCCACGCC CTGCTGGTGATTGGTGGCTTTGAGGCCTACGAGGGTGTGCTGCAGCTGGTGGAGGCCCGGGGGCGCTACG AGGAACTCTGCATTGTCATGTGCGTCATCCCAGCCACCATCAGCAACAATGTGCCTGGCACTGACTTCAG CCTGGGCTCAGACACGGCTGTCAACGCTGCAATGGAGAGTTGTGATCGCATCAAGCAGTCAGCCTCAGGG ACAAAGCGGCGTGTGTTCATTGTAGAGACCATGGGGGGCTACTGTGGCTACCTGGCCACTGTGACCGGCA TTGCTGTGGGTGCCGATGCTGCCTACGTCTTTGAGGACCCTTTCAACATCCATGACTTAAAGGCCAATGT GGAGCATATGACAGAGAAGATGAAGACAGACATCCAGAGGGGACTGGTGCTCCGGAACGAGAAGTGTCAC GAACACTACACCACAGAGTTCCTATACAACCTGTACTCCTCAGAAGGCAGGGGCGTGTTTGACTGCAGGA CCAATGTGCTGGGCCACTTACAGCAGGGTGGTGCTCCAACCCCTTTCGACCGGAACTATGGGACCAAACT GGGGGTGAAGGCCATGTTGTGGGTGTCTGAGAAGCTACGTGATGTCTACCGTAAAGGGCGGGTGTTTGCC AATGCTCCAGACTCAGCCTGTGTGATCGGCCTGCGGAAGAAGGTAGTGGCCTTCAGTCCGGTCACAGAAC TCAAGAAAGAGACTGATTTTGAGCACCGCATGCCCCGGGAGCAGTGGTGGCTGAATCTGCGGCTGATGCT GAAGATGCTGGCACACTATCGCATCAGCATGGCAGACTATGTGTCTGGGGAGCTGGAGCACGTCACACGC CGCACCTTGAGCATAGACAAGGGTTTCTGAGCTTACTGTCACTACTGTTCCTGCGTTCCCGGACTCTCAC CCTCCCAGTGCTAGCCACAGATCCCAGCACCGAGGGGAACTCAAGGGGAACTCGGAGCCCGGGGCTGGCT GAGGGATGTGGGGGTCTACTGTGCTGCTCTGGGGCTCTTCCCTTAGCCCTCCCCCCACCCCCAGGACAGA GAGCCTGTGGGGCCCCTACCTTCCAGTCAGACCCACATTGCCTCTCCCTCACTGGCCAGCTGGCTGCCTC ACCACACGGCTGAAAAGAGCCTCACTTTTTTAGAAATAAAGTCACCTGTCTAGAGCAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:4014395 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4014395 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11358 same embryo
	EMAGE:11356 same embryo
	EMAGE:11357 same embryo
	EurExpress:euxassay_006644 same experiment
	MGI:4827164 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source